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Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application

One of the main hurdles to improving scaffolds for regenerative medicine is the development of non-invasive methods to monitor cell proliferation within three-dimensional environments. Recently, an electrical impedance-based approach has been identified as promising for three-dimensional proliferati...

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Autores principales: Tonello, Sarah, Bianchetti, Andrea, Braga, Simona, Almici, Camillo, Marini, Mirella, Piovani, Giovanna, Guindani, Michele, Dey, Kamol, Sartore, Luciana, Re, Federica, Russo, Domenico, Cantù, Edoardo, Francesco Lopomo, Nicola, Serpelloni, Mauro, Sardini, Emilio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287852/
https://www.ncbi.nlm.nih.gov/pubmed/32413993
http://dx.doi.org/10.3390/ma13102231
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author Tonello, Sarah
Bianchetti, Andrea
Braga, Simona
Almici, Camillo
Marini, Mirella
Piovani, Giovanna
Guindani, Michele
Dey, Kamol
Sartore, Luciana
Re, Federica
Russo, Domenico
Cantù, Edoardo
Francesco Lopomo, Nicola
Serpelloni, Mauro
Sardini, Emilio
author_facet Tonello, Sarah
Bianchetti, Andrea
Braga, Simona
Almici, Camillo
Marini, Mirella
Piovani, Giovanna
Guindani, Michele
Dey, Kamol
Sartore, Luciana
Re, Federica
Russo, Domenico
Cantù, Edoardo
Francesco Lopomo, Nicola
Serpelloni, Mauro
Sardini, Emilio
author_sort Tonello, Sarah
collection PubMed
description One of the main hurdles to improving scaffolds for regenerative medicine is the development of non-invasive methods to monitor cell proliferation within three-dimensional environments. Recently, an electrical impedance-based approach has been identified as promising for three-dimensional proliferation assays. A low-cost impedance-based solution, easily integrable with multi-well plates, is here presented. Sensors were developed using biocompatible carbon-based ink on foldable polyimide substrates by means of a novel aerosol jet printing technique. The setup was tested to monitor the proliferation of human mesenchymal stromal cells into previously validated gelatin-chitosan hybrid hydrogel scaffolds. Reliability of the methodology was assessed comparing variations of the electrical impedance parameters with the outcomes of enzymatic proliferation assay. Results obtained showed a magnitude increase and a phase angle decrease at 4 kHz (maximum of 2.5 kΩ and −9 degrees) and an exponential increase of the modeled resistance and capacitance components due to the cell proliferation (maximum of 1.5 kΩ and 200 nF). A statistically significant relationship with enzymatic assay outcomes could be detected for both phase angle and electric model parameters. Overall, these findings support the potentiality of this non-invasive approach for continuous monitoring of scaffold-based cultures, being also promising in the perspective of optimizing the scaffold-culture system.
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spelling pubmed-72878522020-06-15 Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application Tonello, Sarah Bianchetti, Andrea Braga, Simona Almici, Camillo Marini, Mirella Piovani, Giovanna Guindani, Michele Dey, Kamol Sartore, Luciana Re, Federica Russo, Domenico Cantù, Edoardo Francesco Lopomo, Nicola Serpelloni, Mauro Sardini, Emilio Materials (Basel) Article One of the main hurdles to improving scaffolds for regenerative medicine is the development of non-invasive methods to monitor cell proliferation within three-dimensional environments. Recently, an electrical impedance-based approach has been identified as promising for three-dimensional proliferation assays. A low-cost impedance-based solution, easily integrable with multi-well plates, is here presented. Sensors were developed using biocompatible carbon-based ink on foldable polyimide substrates by means of a novel aerosol jet printing technique. The setup was tested to monitor the proliferation of human mesenchymal stromal cells into previously validated gelatin-chitosan hybrid hydrogel scaffolds. Reliability of the methodology was assessed comparing variations of the electrical impedance parameters with the outcomes of enzymatic proliferation assay. Results obtained showed a magnitude increase and a phase angle decrease at 4 kHz (maximum of 2.5 kΩ and −9 degrees) and an exponential increase of the modeled resistance and capacitance components due to the cell proliferation (maximum of 1.5 kΩ and 200 nF). A statistically significant relationship with enzymatic assay outcomes could be detected for both phase angle and electric model parameters. Overall, these findings support the potentiality of this non-invasive approach for continuous monitoring of scaffold-based cultures, being also promising in the perspective of optimizing the scaffold-culture system. MDPI 2020-05-13 /pmc/articles/PMC7287852/ /pubmed/32413993 http://dx.doi.org/10.3390/ma13102231 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tonello, Sarah
Bianchetti, Andrea
Braga, Simona
Almici, Camillo
Marini, Mirella
Piovani, Giovanna
Guindani, Michele
Dey, Kamol
Sartore, Luciana
Re, Federica
Russo, Domenico
Cantù, Edoardo
Francesco Lopomo, Nicola
Serpelloni, Mauro
Sardini, Emilio
Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application
title Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application
title_full Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application
title_fullStr Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application
title_full_unstemmed Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application
title_short Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application
title_sort impedance-based monitoring of mesenchymal stromal cell three-dimensional proliferation using aerosol jet printed sensors: a tissue engineering application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287852/
https://www.ncbi.nlm.nih.gov/pubmed/32413993
http://dx.doi.org/10.3390/ma13102231
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