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Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release

Protein–peptide–calcium phosphate composites were developed for achieving sustainable and controlled protein release. Bovine serum albumin (BSA) as a model acidic protein was efficiently encapsulated with basic polypeptides such as polylysine and polyarginine during the precipitation of calcium phos...

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Autores principales: Kato, Katsuya, Lee, Sungho, Nagata, Fukue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287863/
https://www.ncbi.nlm.nih.gov/pubmed/32423135
http://dx.doi.org/10.3390/molecules25102312
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author Kato, Katsuya
Lee, Sungho
Nagata, Fukue
author_facet Kato, Katsuya
Lee, Sungho
Nagata, Fukue
author_sort Kato, Katsuya
collection PubMed
description Protein–peptide–calcium phosphate composites were developed for achieving sustainable and controlled protein release. Bovine serum albumin (BSA) as a model acidic protein was efficiently encapsulated with basic polypeptides such as polylysine and polyarginine during the precipitation of calcium phosphate (CaP). The prepared composites were fully characterized in terms of their morphologies, crystallinities, and the porosity of their structures, and from these analyses, it was observed that there are no significant differences between the composites. Scanning transmission electron microscopy and energy dispersive X-ray spectroscopy analysis indicated a homogeneous distribution of nitrogen and sulfur, confirming the uniform distribution of BSA and polypeptide in the CaP composite. In vitro release studies demonstrated that the composite prepared with the peptides α-polylysine and polyarginine were suitable for the gradual release of the protein BSA, while those containing ε-polylysine and no peptide were unsuitable for protein release. Additionally, these composites showed high hemocompatibility for mouse red blood cells, and the osteoblast-like cell proliferation and spread in media with the composites prepared using BSA and α-polylysine showed similar tendencies to medium with no composite. From these results, protein–peptide–CaP composites are expected to be useful as highly biocompatible protein delivery agents.
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spelling pubmed-72878632020-06-15 Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release Kato, Katsuya Lee, Sungho Nagata, Fukue Molecules Article Protein–peptide–calcium phosphate composites were developed for achieving sustainable and controlled protein release. Bovine serum albumin (BSA) as a model acidic protein was efficiently encapsulated with basic polypeptides such as polylysine and polyarginine during the precipitation of calcium phosphate (CaP). The prepared composites were fully characterized in terms of their morphologies, crystallinities, and the porosity of their structures, and from these analyses, it was observed that there are no significant differences between the composites. Scanning transmission electron microscopy and energy dispersive X-ray spectroscopy analysis indicated a homogeneous distribution of nitrogen and sulfur, confirming the uniform distribution of BSA and polypeptide in the CaP composite. In vitro release studies demonstrated that the composite prepared with the peptides α-polylysine and polyarginine were suitable for the gradual release of the protein BSA, while those containing ε-polylysine and no peptide were unsuitable for protein release. Additionally, these composites showed high hemocompatibility for mouse red blood cells, and the osteoblast-like cell proliferation and spread in media with the composites prepared using BSA and α-polylysine showed similar tendencies to medium with no composite. From these results, protein–peptide–CaP composites are expected to be useful as highly biocompatible protein delivery agents. MDPI 2020-05-14 /pmc/articles/PMC7287863/ /pubmed/32423135 http://dx.doi.org/10.3390/molecules25102312 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kato, Katsuya
Lee, Sungho
Nagata, Fukue
Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release
title Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release
title_full Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release
title_fullStr Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release
title_full_unstemmed Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release
title_short Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release
title_sort preparation of protein–peptide–calcium phosphate composites for controlled protein release
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287863/
https://www.ncbi.nlm.nih.gov/pubmed/32423135
http://dx.doi.org/10.3390/molecules25102312
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