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Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts

Anacamptis pyramidalis (L.) Rich. forms part of the Orchidaceae family that is highly valued for its horticultural as well as therapeutic benefits. The present study set out to investigate the inhibitory activity of A. pyramidalis tubers against key biological targets for the management of type 2 di...

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Autores principales: Fawzi Mahomoodally, Mohamad, Picot-Allain, Marie Carene Nancy, Zengin, Gokhan, Llorent-Martínez, Eulogio J., Abdullah, Hassan H., Ak, Gunes, Senkardes, Ismail, Chiavaroli, Annalisa, Menghini, Luigi, Recinella, Lucia, Brunetti, Luigi, Leone, Sheila, Orlando, Giustino, Ferrante, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288046/
https://www.ncbi.nlm.nih.gov/pubmed/32455936
http://dx.doi.org/10.3390/molecules25102422
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author Fawzi Mahomoodally, Mohamad
Picot-Allain, Marie Carene Nancy
Zengin, Gokhan
Llorent-Martínez, Eulogio J.
Abdullah, Hassan H.
Ak, Gunes
Senkardes, Ismail
Chiavaroli, Annalisa
Menghini, Luigi
Recinella, Lucia
Brunetti, Luigi
Leone, Sheila
Orlando, Giustino
Ferrante, Claudio
author_facet Fawzi Mahomoodally, Mohamad
Picot-Allain, Marie Carene Nancy
Zengin, Gokhan
Llorent-Martínez, Eulogio J.
Abdullah, Hassan H.
Ak, Gunes
Senkardes, Ismail
Chiavaroli, Annalisa
Menghini, Luigi
Recinella, Lucia
Brunetti, Luigi
Leone, Sheila
Orlando, Giustino
Ferrante, Claudio
author_sort Fawzi Mahomoodally, Mohamad
collection PubMed
description Anacamptis pyramidalis (L.) Rich. forms part of the Orchidaceae family that is highly valued for its horticultural as well as therapeutic benefits. The present study set out to investigate the inhibitory activity of A. pyramidalis tubers against key biological targets for the management of type 2 diabetes, Alzheimer disease, and skin hyperpigmentation. In addition, the antioxidant potential of the extracts was also assessed using multiple methods. The detailed phytochemical profiles of the extracts were determined using high-performance liquid chromatography. Based on qualitative phytochemical fingerprint, a network pharmacology analysis was conducted as well. Parishin was identified from the water extract only, whereas gastrodin and caffeic acid derivatives were present in the methanol extract. The methanol extract exhibited high inhibitory activity against tyrosinase (69.69 mg kojic acid equivalent/g extract), α-amylase (15.76 mg acarbose equivalent/g extract), and α-glucosidase (20.07 mg acarbose equivalent/g extract). Similarly, the methanol extract showed highest antioxidant potential (22.12, 44.23, 45.56, and 29.38 mg Trolox equivalent/g extract, for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), CUPric Reducing Antioxidant Capacity (CUPRAC), and Ferric Reducing Antioxidant Power (FRAP) assays, respectively). Finally, the results of network pharmacology analysis, besides corroborating traditional uses of plant extracts in the management of cold and flu, confirmed a direct involvement of identified phytochemicals in the observed enzyme inhibitory effects, especially against tyrosinase, α-amylase, and α-glucosidase. Furthermore, based on the results of both colorimetric assays and network pharmacology analysis related to the activity of A. pyramidalis extracts and identified phytocompounds on enzymes involved in type 2 diabetes, a docking study was conducted in order to investigate the putative interactions of oxo-dihydroxy octadecenoic acid trihydroxy octadecenoic acid against aldose reductase, peroxisome proliferator-activated receptor (PPAR)-α, dipeptidyl peptidase (DPP)-IV, and α-glucosidase. Docking analysis suggested the inhibitory activity of these compounds against the aforementioned enzymes, with a better inhibitory profile shown by oxo-dihydroxy octadecenoic acid. Overall, the present findings supported the rationale for the use of A. pyramidalis as source of bioactive metabolites and highlight, today more than ever, for the strong necessity of linkage strategy between wild resource valorization and conservation policy.
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spelling pubmed-72880462020-06-15 Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts Fawzi Mahomoodally, Mohamad Picot-Allain, Marie Carene Nancy Zengin, Gokhan Llorent-Martínez, Eulogio J. Abdullah, Hassan H. Ak, Gunes Senkardes, Ismail Chiavaroli, Annalisa Menghini, Luigi Recinella, Lucia Brunetti, Luigi Leone, Sheila Orlando, Giustino Ferrante, Claudio Molecules Article Anacamptis pyramidalis (L.) Rich. forms part of the Orchidaceae family that is highly valued for its horticultural as well as therapeutic benefits. The present study set out to investigate the inhibitory activity of A. pyramidalis tubers against key biological targets for the management of type 2 diabetes, Alzheimer disease, and skin hyperpigmentation. In addition, the antioxidant potential of the extracts was also assessed using multiple methods. The detailed phytochemical profiles of the extracts were determined using high-performance liquid chromatography. Based on qualitative phytochemical fingerprint, a network pharmacology analysis was conducted as well. Parishin was identified from the water extract only, whereas gastrodin and caffeic acid derivatives were present in the methanol extract. The methanol extract exhibited high inhibitory activity against tyrosinase (69.69 mg kojic acid equivalent/g extract), α-amylase (15.76 mg acarbose equivalent/g extract), and α-glucosidase (20.07 mg acarbose equivalent/g extract). Similarly, the methanol extract showed highest antioxidant potential (22.12, 44.23, 45.56, and 29.38 mg Trolox equivalent/g extract, for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), CUPric Reducing Antioxidant Capacity (CUPRAC), and Ferric Reducing Antioxidant Power (FRAP) assays, respectively). Finally, the results of network pharmacology analysis, besides corroborating traditional uses of plant extracts in the management of cold and flu, confirmed a direct involvement of identified phytochemicals in the observed enzyme inhibitory effects, especially against tyrosinase, α-amylase, and α-glucosidase. Furthermore, based on the results of both colorimetric assays and network pharmacology analysis related to the activity of A. pyramidalis extracts and identified phytocompounds on enzymes involved in type 2 diabetes, a docking study was conducted in order to investigate the putative interactions of oxo-dihydroxy octadecenoic acid trihydroxy octadecenoic acid against aldose reductase, peroxisome proliferator-activated receptor (PPAR)-α, dipeptidyl peptidase (DPP)-IV, and α-glucosidase. Docking analysis suggested the inhibitory activity of these compounds against the aforementioned enzymes, with a better inhibitory profile shown by oxo-dihydroxy octadecenoic acid. Overall, the present findings supported the rationale for the use of A. pyramidalis as source of bioactive metabolites and highlight, today more than ever, for the strong necessity of linkage strategy between wild resource valorization and conservation policy. MDPI 2020-05-22 /pmc/articles/PMC7288046/ /pubmed/32455936 http://dx.doi.org/10.3390/molecules25102422 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fawzi Mahomoodally, Mohamad
Picot-Allain, Marie Carene Nancy
Zengin, Gokhan
Llorent-Martínez, Eulogio J.
Abdullah, Hassan H.
Ak, Gunes
Senkardes, Ismail
Chiavaroli, Annalisa
Menghini, Luigi
Recinella, Lucia
Brunetti, Luigi
Leone, Sheila
Orlando, Giustino
Ferrante, Claudio
Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts
title Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts
title_full Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts
title_fullStr Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts
title_full_unstemmed Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts
title_short Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts
title_sort phytochemical analysis, network pharmacology and in silico investigations on anacamptis pyramidalis tuber extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288046/
https://www.ncbi.nlm.nih.gov/pubmed/32455936
http://dx.doi.org/10.3390/molecules25102422
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