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An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants

Silica-based ceramics have been proposed for coating purposes to enhance dental and orthopedic titanium (Ti) implant bioactivity. The aim of this study was to investigate the influence of sphene-based bioceramic (CaO.TiO(2).SiO(2)) coatings on implant osseointegration in vivo. Sphene coatings were o...

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Autores principales: Brunello, Giulia, Biasetto, Lisa, Elsayed, Hamada, Sbettega, Elia, Gardin, Chiara, Scanu, Anna, Carmignato, Simone, Zavan, Barbara, Sivolella, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288333/
https://www.ncbi.nlm.nih.gov/pubmed/32365687
http://dx.doi.org/10.3390/jcm9051290
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author Brunello, Giulia
Biasetto, Lisa
Elsayed, Hamada
Sbettega, Elia
Gardin, Chiara
Scanu, Anna
Carmignato, Simone
Zavan, Barbara
Sivolella, Stefano
author_facet Brunello, Giulia
Biasetto, Lisa
Elsayed, Hamada
Sbettega, Elia
Gardin, Chiara
Scanu, Anna
Carmignato, Simone
Zavan, Barbara
Sivolella, Stefano
author_sort Brunello, Giulia
collection PubMed
description Silica-based ceramics have been proposed for coating purposes to enhance dental and orthopedic titanium (Ti) implant bioactivity. The aim of this study was to investigate the influence of sphene-based bioceramic (CaO.TiO(2).SiO(2)) coatings on implant osseointegration in vivo. Sphene coatings were obtained from preceramic polymers and nano-sized active precursors and deposited by an automatic airbrush. Twenty customized Ti implants, ten sphene-coated and ten uncoated rough implants were implanted into the proximal femurs of ten Sprague-Dawley rats. Overall, cortical and cancellous bone-to-implant contact (BIC) were determined using micro-computed tomography (micro-CT) at 14 and 28 days. Moreover, peri-implant bone healing was histologically and histomorphometrically evaluated. The white blood cell count in the synovial fluid of the knee joints, if present, was also assessed. No difference in the BIC values was observed between the sphene-coated and uncoated implants, overall and in the two bone compartments (p > 0.05). Delamination of the coating occurred in three cases. Consistently with micro-CT data, the histological evaluation revealed no differences between the two groups. In addition, no synovial fluid could be collected on the test side, thus confirming sphene biocompatibility. In conclusion, sphene coating was found to be a suitable material for biomedical applications. Further studies are needed to improve coating adhesion to the implants.
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spelling pubmed-72883332020-06-17 An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants Brunello, Giulia Biasetto, Lisa Elsayed, Hamada Sbettega, Elia Gardin, Chiara Scanu, Anna Carmignato, Simone Zavan, Barbara Sivolella, Stefano J Clin Med Article Silica-based ceramics have been proposed for coating purposes to enhance dental and orthopedic titanium (Ti) implant bioactivity. The aim of this study was to investigate the influence of sphene-based bioceramic (CaO.TiO(2).SiO(2)) coatings on implant osseointegration in vivo. Sphene coatings were obtained from preceramic polymers and nano-sized active precursors and deposited by an automatic airbrush. Twenty customized Ti implants, ten sphene-coated and ten uncoated rough implants were implanted into the proximal femurs of ten Sprague-Dawley rats. Overall, cortical and cancellous bone-to-implant contact (BIC) were determined using micro-computed tomography (micro-CT) at 14 and 28 days. Moreover, peri-implant bone healing was histologically and histomorphometrically evaluated. The white blood cell count in the synovial fluid of the knee joints, if present, was also assessed. No difference in the BIC values was observed between the sphene-coated and uncoated implants, overall and in the two bone compartments (p > 0.05). Delamination of the coating occurred in three cases. Consistently with micro-CT data, the histological evaluation revealed no differences between the two groups. In addition, no synovial fluid could be collected on the test side, thus confirming sphene biocompatibility. In conclusion, sphene coating was found to be a suitable material for biomedical applications. Further studies are needed to improve coating adhesion to the implants. MDPI 2020-04-29 /pmc/articles/PMC7288333/ /pubmed/32365687 http://dx.doi.org/10.3390/jcm9051290 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brunello, Giulia
Biasetto, Lisa
Elsayed, Hamada
Sbettega, Elia
Gardin, Chiara
Scanu, Anna
Carmignato, Simone
Zavan, Barbara
Sivolella, Stefano
An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants
title An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants
title_full An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants
title_fullStr An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants
title_full_unstemmed An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants
title_short An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants
title_sort in vivo study in rat femurs of bioactive silicate coatings on titanium dental implants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288333/
https://www.ncbi.nlm.nih.gov/pubmed/32365687
http://dx.doi.org/10.3390/jcm9051290
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