Cargando…
An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants
Silica-based ceramics have been proposed for coating purposes to enhance dental and orthopedic titanium (Ti) implant bioactivity. The aim of this study was to investigate the influence of sphene-based bioceramic (CaO.TiO(2).SiO(2)) coatings on implant osseointegration in vivo. Sphene coatings were o...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288333/ https://www.ncbi.nlm.nih.gov/pubmed/32365687 http://dx.doi.org/10.3390/jcm9051290 |
_version_ | 1783545255134494720 |
---|---|
author | Brunello, Giulia Biasetto, Lisa Elsayed, Hamada Sbettega, Elia Gardin, Chiara Scanu, Anna Carmignato, Simone Zavan, Barbara Sivolella, Stefano |
author_facet | Brunello, Giulia Biasetto, Lisa Elsayed, Hamada Sbettega, Elia Gardin, Chiara Scanu, Anna Carmignato, Simone Zavan, Barbara Sivolella, Stefano |
author_sort | Brunello, Giulia |
collection | PubMed |
description | Silica-based ceramics have been proposed for coating purposes to enhance dental and orthopedic titanium (Ti) implant bioactivity. The aim of this study was to investigate the influence of sphene-based bioceramic (CaO.TiO(2).SiO(2)) coatings on implant osseointegration in vivo. Sphene coatings were obtained from preceramic polymers and nano-sized active precursors and deposited by an automatic airbrush. Twenty customized Ti implants, ten sphene-coated and ten uncoated rough implants were implanted into the proximal femurs of ten Sprague-Dawley rats. Overall, cortical and cancellous bone-to-implant contact (BIC) were determined using micro-computed tomography (micro-CT) at 14 and 28 days. Moreover, peri-implant bone healing was histologically and histomorphometrically evaluated. The white blood cell count in the synovial fluid of the knee joints, if present, was also assessed. No difference in the BIC values was observed between the sphene-coated and uncoated implants, overall and in the two bone compartments (p > 0.05). Delamination of the coating occurred in three cases. Consistently with micro-CT data, the histological evaluation revealed no differences between the two groups. In addition, no synovial fluid could be collected on the test side, thus confirming sphene biocompatibility. In conclusion, sphene coating was found to be a suitable material for biomedical applications. Further studies are needed to improve coating adhesion to the implants. |
format | Online Article Text |
id | pubmed-7288333 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72883332020-06-17 An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants Brunello, Giulia Biasetto, Lisa Elsayed, Hamada Sbettega, Elia Gardin, Chiara Scanu, Anna Carmignato, Simone Zavan, Barbara Sivolella, Stefano J Clin Med Article Silica-based ceramics have been proposed for coating purposes to enhance dental and orthopedic titanium (Ti) implant bioactivity. The aim of this study was to investigate the influence of sphene-based bioceramic (CaO.TiO(2).SiO(2)) coatings on implant osseointegration in vivo. Sphene coatings were obtained from preceramic polymers and nano-sized active precursors and deposited by an automatic airbrush. Twenty customized Ti implants, ten sphene-coated and ten uncoated rough implants were implanted into the proximal femurs of ten Sprague-Dawley rats. Overall, cortical and cancellous bone-to-implant contact (BIC) were determined using micro-computed tomography (micro-CT) at 14 and 28 days. Moreover, peri-implant bone healing was histologically and histomorphometrically evaluated. The white blood cell count in the synovial fluid of the knee joints, if present, was also assessed. No difference in the BIC values was observed between the sphene-coated and uncoated implants, overall and in the two bone compartments (p > 0.05). Delamination of the coating occurred in three cases. Consistently with micro-CT data, the histological evaluation revealed no differences between the two groups. In addition, no synovial fluid could be collected on the test side, thus confirming sphene biocompatibility. In conclusion, sphene coating was found to be a suitable material for biomedical applications. Further studies are needed to improve coating adhesion to the implants. MDPI 2020-04-29 /pmc/articles/PMC7288333/ /pubmed/32365687 http://dx.doi.org/10.3390/jcm9051290 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brunello, Giulia Biasetto, Lisa Elsayed, Hamada Sbettega, Elia Gardin, Chiara Scanu, Anna Carmignato, Simone Zavan, Barbara Sivolella, Stefano An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants |
title | An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants |
title_full | An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants |
title_fullStr | An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants |
title_full_unstemmed | An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants |
title_short | An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants |
title_sort | in vivo study in rat femurs of bioactive silicate coatings on titanium dental implants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288333/ https://www.ncbi.nlm.nih.gov/pubmed/32365687 http://dx.doi.org/10.3390/jcm9051290 |
work_keys_str_mv | AT brunellogiulia aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT biasettolisa aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT elsayedhamada aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT sbettegaelia aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT gardinchiara aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT scanuanna aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT carmignatosimone aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT zavanbarbara aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT sivolellastefano aninvivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT brunellogiulia invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT biasettolisa invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT elsayedhamada invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT sbettegaelia invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT gardinchiara invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT scanuanna invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT carmignatosimone invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT zavanbarbara invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants AT sivolellastefano invivostudyinratfemursofbioactivesilicatecoatingsontitaniumdentalimplants |