Cargando…

Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library

Tn916 is a conjugative transposon (CTn) and the first reported and most well characterised of the Tn916/Tn1545 family of CTns. Tn916-like elements have a characteristic modular structure and different members of this family have been identified based on similarities and variations in these modules....

Descripción completa

Detalles Bibliográficos
Autores principales: Reynolds, Liam J., Anjum, Muna F., Roberts, Adam P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288454/
https://www.ncbi.nlm.nih.gov/pubmed/32422869
http://dx.doi.org/10.3390/genes11050548
_version_ 1783545279870402560
author Reynolds, Liam J.
Anjum, Muna F.
Roberts, Adam P.
author_facet Reynolds, Liam J.
Anjum, Muna F.
Roberts, Adam P.
author_sort Reynolds, Liam J.
collection PubMed
description Tn916 is a conjugative transposon (CTn) and the first reported and most well characterised of the Tn916/Tn1545 family of CTns. Tn916-like elements have a characteristic modular structure and different members of this family have been identified based on similarities and variations in these modules. In addition to carrying genes encoding proteins required for their conjugation, Tn916-like elements also carry accessory, antimicrobial resistance genes; most commonly the tetracycline resistance gene, tet(M). Our study aimed to identify and characterise tetracycline resistance genes from the human saliva metagenome using a functional metagenomic approach. We identified a tetracycline-resistant clone, TT31, the sequencing of which revealed it to encode both tet(M) and tet(L). Comparison of the TT31 sequence with the accessory, regulation, and recombination modules of other Tn916-like elements indicated that a partial Tn916-like element encoding a truncated orf9 was cloned in TT31. Analysis indicated that a previous insertion within the truncated orf9 created the full length orf9 found in most Tn916-like transposons; demonstrating that orf9 is, in fact, the result of a gene fusion event. Thus, we hypothesise that the Tn916-like element cloned in TT31 likely represents an ancestral Tn916.
format Online
Article
Text
id pubmed-7288454
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72884542020-06-17 Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library Reynolds, Liam J. Anjum, Muna F. Roberts, Adam P. Genes (Basel) Article Tn916 is a conjugative transposon (CTn) and the first reported and most well characterised of the Tn916/Tn1545 family of CTns. Tn916-like elements have a characteristic modular structure and different members of this family have been identified based on similarities and variations in these modules. In addition to carrying genes encoding proteins required for their conjugation, Tn916-like elements also carry accessory, antimicrobial resistance genes; most commonly the tetracycline resistance gene, tet(M). Our study aimed to identify and characterise tetracycline resistance genes from the human saliva metagenome using a functional metagenomic approach. We identified a tetracycline-resistant clone, TT31, the sequencing of which revealed it to encode both tet(M) and tet(L). Comparison of the TT31 sequence with the accessory, regulation, and recombination modules of other Tn916-like elements indicated that a partial Tn916-like element encoding a truncated orf9 was cloned in TT31. Analysis indicated that a previous insertion within the truncated orf9 created the full length orf9 found in most Tn916-like transposons; demonstrating that orf9 is, in fact, the result of a gene fusion event. Thus, we hypothesise that the Tn916-like element cloned in TT31 likely represents an ancestral Tn916. MDPI 2020-05-14 /pmc/articles/PMC7288454/ /pubmed/32422869 http://dx.doi.org/10.3390/genes11050548 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reynolds, Liam J.
Anjum, Muna F.
Roberts, Adam P.
Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library
title Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library
title_full Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library
title_fullStr Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library
title_full_unstemmed Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library
title_short Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library
title_sort detection of a novel, and likely ancestral, tn916-like element from a human saliva metagenomic library
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288454/
https://www.ncbi.nlm.nih.gov/pubmed/32422869
http://dx.doi.org/10.3390/genes11050548
work_keys_str_mv AT reynoldsliamj detectionofanovelandlikelyancestraltn916likeelementfromahumansalivametagenomiclibrary
AT anjummunaf detectionofanovelandlikelyancestraltn916likeelementfromahumansalivametagenomiclibrary
AT robertsadamp detectionofanovelandlikelyancestraltn916likeelementfromahumansalivametagenomiclibrary