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Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice

A colon cancer growth inhibitor partially purified from the isolated cell wall membrane fraction of Chlorella sorokiniana, here referred to as Chlorella membrane factor (CMF), was evaluated for its antitumor and immunomodulatory effects in cell culture and in a colon carcinoma mouse model. The CMF t...

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Detalles Bibliográficos
Autores principales: Ishiguro, Susumu, Robben, Nicole, Burghart, Riley, Cote, Paige, Greenway, Sarah, Thakkar, Ravindra, Upreti, Deepa, Nakashima, Ayaka, Suzuki, Kengo, Comer, Jeffrey, Tamura, Masaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288830/
https://www.ncbi.nlm.nih.gov/pubmed/32009489
http://dx.doi.org/10.1177/1534735419900555
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author Ishiguro, Susumu
Robben, Nicole
Burghart, Riley
Cote, Paige
Greenway, Sarah
Thakkar, Ravindra
Upreti, Deepa
Nakashima, Ayaka
Suzuki, Kengo
Comer, Jeffrey
Tamura, Masaaki
author_facet Ishiguro, Susumu
Robben, Nicole
Burghart, Riley
Cote, Paige
Greenway, Sarah
Thakkar, Ravindra
Upreti, Deepa
Nakashima, Ayaka
Suzuki, Kengo
Comer, Jeffrey
Tamura, Masaaki
author_sort Ishiguro, Susumu
collection PubMed
description A colon cancer growth inhibitor partially purified from the isolated cell wall membrane fraction of Chlorella sorokiniana, here referred to as Chlorella membrane factor (CMF), was evaluated for its antitumor and immunomodulatory effects in cell culture and in a colon carcinoma mouse model. The CMF treatment dose- and time-dependently inhibited colon carcinoma cell growth in 2-dimensional cultures. Treatment with CMF also significantly inhibited the growth of colon carcinoma spheroids in 3-dimensional cell culture in coculture with T lymphocytes. In a mouse CT26 colon carcinoma peritoneal dissemination model, intraperitoneal injection of CMF (10 or 30 mg dry weight/kg body weight, every other day) dose-dependently and significantly attenuated the growth of tumor nodules via induction of tumor cell apoptosis. Evaluation of immune cell populations in ascites showed that CMF treatment tended to increase T lymphocytes but lower granulocyte populations. The present study suggests that the cell wall membrane fraction of Chlorella sorokiniana contains a bioactive material that inhibits colon carcinoma growth via direct cell growth inhibition and stimulation of host antitumor immunity. Hence, it is suggested that the Chlorella cell wall membrane extract or a bioactive substance in the extract is an attractive complementary medicine for cancer therapy.
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spelling pubmed-72888302020-06-22 Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice Ishiguro, Susumu Robben, Nicole Burghart, Riley Cote, Paige Greenway, Sarah Thakkar, Ravindra Upreti, Deepa Nakashima, Ayaka Suzuki, Kengo Comer, Jeffrey Tamura, Masaaki Integr Cancer Ther Research Article A colon cancer growth inhibitor partially purified from the isolated cell wall membrane fraction of Chlorella sorokiniana, here referred to as Chlorella membrane factor (CMF), was evaluated for its antitumor and immunomodulatory effects in cell culture and in a colon carcinoma mouse model. The CMF treatment dose- and time-dependently inhibited colon carcinoma cell growth in 2-dimensional cultures. Treatment with CMF also significantly inhibited the growth of colon carcinoma spheroids in 3-dimensional cell culture in coculture with T lymphocytes. In a mouse CT26 colon carcinoma peritoneal dissemination model, intraperitoneal injection of CMF (10 or 30 mg dry weight/kg body weight, every other day) dose-dependently and significantly attenuated the growth of tumor nodules via induction of tumor cell apoptosis. Evaluation of immune cell populations in ascites showed that CMF treatment tended to increase T lymphocytes but lower granulocyte populations. The present study suggests that the cell wall membrane fraction of Chlorella sorokiniana contains a bioactive material that inhibits colon carcinoma growth via direct cell growth inhibition and stimulation of host antitumor immunity. Hence, it is suggested that the Chlorella cell wall membrane extract or a bioactive substance in the extract is an attractive complementary medicine for cancer therapy. SAGE Publications 2020-02-01 /pmc/articles/PMC7288830/ /pubmed/32009489 http://dx.doi.org/10.1177/1534735419900555 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
Ishiguro, Susumu
Robben, Nicole
Burghart, Riley
Cote, Paige
Greenway, Sarah
Thakkar, Ravindra
Upreti, Deepa
Nakashima, Ayaka
Suzuki, Kengo
Comer, Jeffrey
Tamura, Masaaki
Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice
title Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice
title_full Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice
title_fullStr Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice
title_full_unstemmed Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice
title_short Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice
title_sort cell wall membrane fraction of chlorella sorokiniana enhances host antitumor immunity and inhibits colon carcinoma growth in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288830/
https://www.ncbi.nlm.nih.gov/pubmed/32009489
http://dx.doi.org/10.1177/1534735419900555
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