Cargando…
Physicochemical considerations for bottom-up synthetic biology
The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic stre...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289010/ https://www.ncbi.nlm.nih.gov/pubmed/33523159 http://dx.doi.org/10.1042/ETLS20190017 |
_version_ | 1783545385832153088 |
---|---|
author | Śmigiel, Wojciech Mikołaj Lefrançois, Pauline Poolman, Bert |
author_facet | Śmigiel, Wojciech Mikołaj Lefrançois, Pauline Poolman, Bert |
author_sort | Śmigiel, Wojciech Mikołaj |
collection | PubMed |
description | The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic strength, (macro)molecular crowding, redox state and metabolic energy conservation. These physicochemical parameters influence protein activity and need to be maintained within limits to ensure the system remains in steady-state. We present the physicochemical considerations for building synthetic cells with dimensions ranging from the smallest prokaryotes to eukaryotic cells. |
format | Online Article Text |
id | pubmed-7289010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72890102020-06-18 Physicochemical considerations for bottom-up synthetic biology Śmigiel, Wojciech Mikołaj Lefrançois, Pauline Poolman, Bert Emerg Top Life Sci Review Articles The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic strength, (macro)molecular crowding, redox state and metabolic energy conservation. These physicochemical parameters influence protein activity and need to be maintained within limits to ensure the system remains in steady-state. We present the physicochemical considerations for building synthetic cells with dimensions ranging from the smallest prokaryotes to eukaryotic cells. Portland Press Ltd. 2019-11-11 2019-08-28 /pmc/articles/PMC7289010/ /pubmed/33523159 http://dx.doi.org/10.1042/ETLS20190017 Text en © 2019 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and the Royal Society of Biology and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Articles Śmigiel, Wojciech Mikołaj Lefrançois, Pauline Poolman, Bert Physicochemical considerations for bottom-up synthetic biology |
title | Physicochemical considerations for bottom-up synthetic biology |
title_full | Physicochemical considerations for bottom-up synthetic biology |
title_fullStr | Physicochemical considerations for bottom-up synthetic biology |
title_full_unstemmed | Physicochemical considerations for bottom-up synthetic biology |
title_short | Physicochemical considerations for bottom-up synthetic biology |
title_sort | physicochemical considerations for bottom-up synthetic biology |
topic | Review Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289010/ https://www.ncbi.nlm.nih.gov/pubmed/33523159 http://dx.doi.org/10.1042/ETLS20190017 |
work_keys_str_mv | AT smigielwojciechmikołaj physicochemicalconsiderationsforbottomupsyntheticbiology AT lefrancoispauline physicochemicalconsiderationsforbottomupsyntheticbiology AT poolmanbert physicochemicalconsiderationsforbottomupsyntheticbiology |