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Physicochemical considerations for bottom-up synthetic biology

The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic stre...

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Detalles Bibliográficos
Autores principales: Śmigiel, Wojciech Mikołaj, Lefrançois, Pauline, Poolman, Bert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289010/
https://www.ncbi.nlm.nih.gov/pubmed/33523159
http://dx.doi.org/10.1042/ETLS20190017
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author Śmigiel, Wojciech Mikołaj
Lefrançois, Pauline
Poolman, Bert
author_facet Śmigiel, Wojciech Mikołaj
Lefrançois, Pauline
Poolman, Bert
author_sort Śmigiel, Wojciech Mikołaj
collection PubMed
description The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic strength, (macro)molecular crowding, redox state and metabolic energy conservation. These physicochemical parameters influence protein activity and need to be maintained within limits to ensure the system remains in steady-state. We present the physicochemical considerations for building synthetic cells with dimensions ranging from the smallest prokaryotes to eukaryotic cells.
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spelling pubmed-72890102020-06-18 Physicochemical considerations for bottom-up synthetic biology Śmigiel, Wojciech Mikołaj Lefrançois, Pauline Poolman, Bert Emerg Top Life Sci Review Articles The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic strength, (macro)molecular crowding, redox state and metabolic energy conservation. These physicochemical parameters influence protein activity and need to be maintained within limits to ensure the system remains in steady-state. We present the physicochemical considerations for building synthetic cells with dimensions ranging from the smallest prokaryotes to eukaryotic cells. Portland Press Ltd. 2019-11-11 2019-08-28 /pmc/articles/PMC7289010/ /pubmed/33523159 http://dx.doi.org/10.1042/ETLS20190017 Text en © 2019 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and the Royal Society of Biology and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Articles
Śmigiel, Wojciech Mikołaj
Lefrançois, Pauline
Poolman, Bert
Physicochemical considerations for bottom-up synthetic biology
title Physicochemical considerations for bottom-up synthetic biology
title_full Physicochemical considerations for bottom-up synthetic biology
title_fullStr Physicochemical considerations for bottom-up synthetic biology
title_full_unstemmed Physicochemical considerations for bottom-up synthetic biology
title_short Physicochemical considerations for bottom-up synthetic biology
title_sort physicochemical considerations for bottom-up synthetic biology
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289010/
https://www.ncbi.nlm.nih.gov/pubmed/33523159
http://dx.doi.org/10.1042/ETLS20190017
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