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Mapping QTLs underpin nutrition components in aromatic rice germplasm

As rice is an important staple food globally, research for development and enhancement of its nutritional value it is an imperative task. Identification of nutrient enriched rice germplasm and exploiting them for breeding programme is the easiest way to develop better quality rice. In this study, we...

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Autores principales: Islam, M. Z., Arifuzzaman, M., Banik, S., Hossain, M. A., Ferdous, J., Khalequzzaman, M., Pittendrigh, B. R., Tomita, M., Ali, M. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289389/
https://www.ncbi.nlm.nih.gov/pubmed/32525930
http://dx.doi.org/10.1371/journal.pone.0234395
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author Islam, M. Z.
Arifuzzaman, M.
Banik, S.
Hossain, M. A.
Ferdous, J.
Khalequzzaman, M.
Pittendrigh, B. R.
Tomita, M.
Ali, M. P.
author_facet Islam, M. Z.
Arifuzzaman, M.
Banik, S.
Hossain, M. A.
Ferdous, J.
Khalequzzaman, M.
Pittendrigh, B. R.
Tomita, M.
Ali, M. P.
author_sort Islam, M. Z.
collection PubMed
description As rice is an important staple food globally, research for development and enhancement of its nutritional value it is an imperative task. Identification of nutrient enriched rice germplasm and exploiting them for breeding programme is the easiest way to develop better quality rice. In this study, we analyzed 113 aromatic rice germplasm in order to identify quantitative trait loci (QTL) underpinning nutrition components and determined by measuring the normal frequency distribution for Fe, Zn, amylose, and protein content in those rice germplasm. Comparatively, the germplasm Radhuni pagal, Kalobakri, Thakurbhog (26.6 ppm) and Hatisail exhibited the highest mean values for Fe (16.9 ppm), Zn (34.1 ppm), amylose (26.6 ppm) and protein content (11.0 ppm), respectively. Moreover, a significant linear relationship (R(2) = 0.693) was observed between Fe and Zn contents. Cluster analysis based on Mahalanobis D(2) distances revealed four major clusters of 113 rice germplasm, with cluster III containing a maximum 37 germplasm and a maximum inter-cluster distance between clusters III and IV. The 45 polymorphic SSRs and four trait associations exhibited eight significant quantitative trait loci (QTL) located on eight different chromosomes using composite interval mapping (CIM). The highly significant QTL (variance 7.89%, LOD 2.02) for protein content (QTL.pro.1) was observed on chromosome 1 at 94.9cM position. Also, four QTLs for amylose content were observed with the highly significant QTL.amy.8 located on chromosome 8 exhibiting 7.2% variance with LOD 1.83. Only one QTL (QTL.Fe.9) for Fe content was located on chromosome 9 (LOD 1.24), and two (QTL.Zn.4 and QTL.Zn.5) for Zn on chromosome 4 (LOD 1.71) and 5 (LOD 1.18), respectively. Overall, germplasm from clusters III and IV might offer higher heterotic response with the identified QTLs playing a significant role in any rice biofortification breeding program and released with development of new varieties.
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spelling pubmed-72893892020-06-15 Mapping QTLs underpin nutrition components in aromatic rice germplasm Islam, M. Z. Arifuzzaman, M. Banik, S. Hossain, M. A. Ferdous, J. Khalequzzaman, M. Pittendrigh, B. R. Tomita, M. Ali, M. P. PLoS One Research Article As rice is an important staple food globally, research for development and enhancement of its nutritional value it is an imperative task. Identification of nutrient enriched rice germplasm and exploiting them for breeding programme is the easiest way to develop better quality rice. In this study, we analyzed 113 aromatic rice germplasm in order to identify quantitative trait loci (QTL) underpinning nutrition components and determined by measuring the normal frequency distribution for Fe, Zn, amylose, and protein content in those rice germplasm. Comparatively, the germplasm Radhuni pagal, Kalobakri, Thakurbhog (26.6 ppm) and Hatisail exhibited the highest mean values for Fe (16.9 ppm), Zn (34.1 ppm), amylose (26.6 ppm) and protein content (11.0 ppm), respectively. Moreover, a significant linear relationship (R(2) = 0.693) was observed between Fe and Zn contents. Cluster analysis based on Mahalanobis D(2) distances revealed four major clusters of 113 rice germplasm, with cluster III containing a maximum 37 germplasm and a maximum inter-cluster distance between clusters III and IV. The 45 polymorphic SSRs and four trait associations exhibited eight significant quantitative trait loci (QTL) located on eight different chromosomes using composite interval mapping (CIM). The highly significant QTL (variance 7.89%, LOD 2.02) for protein content (QTL.pro.1) was observed on chromosome 1 at 94.9cM position. Also, four QTLs for amylose content were observed with the highly significant QTL.amy.8 located on chromosome 8 exhibiting 7.2% variance with LOD 1.83. Only one QTL (QTL.Fe.9) for Fe content was located on chromosome 9 (LOD 1.24), and two (QTL.Zn.4 and QTL.Zn.5) for Zn on chromosome 4 (LOD 1.71) and 5 (LOD 1.18), respectively. Overall, germplasm from clusters III and IV might offer higher heterotic response with the identified QTLs playing a significant role in any rice biofortification breeding program and released with development of new varieties. Public Library of Science 2020-06-11 /pmc/articles/PMC7289389/ /pubmed/32525930 http://dx.doi.org/10.1371/journal.pone.0234395 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Islam, M. Z.
Arifuzzaman, M.
Banik, S.
Hossain, M. A.
Ferdous, J.
Khalequzzaman, M.
Pittendrigh, B. R.
Tomita, M.
Ali, M. P.
Mapping QTLs underpin nutrition components in aromatic rice germplasm
title Mapping QTLs underpin nutrition components in aromatic rice germplasm
title_full Mapping QTLs underpin nutrition components in aromatic rice germplasm
title_fullStr Mapping QTLs underpin nutrition components in aromatic rice germplasm
title_full_unstemmed Mapping QTLs underpin nutrition components in aromatic rice germplasm
title_short Mapping QTLs underpin nutrition components in aromatic rice germplasm
title_sort mapping qtls underpin nutrition components in aromatic rice germplasm
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289389/
https://www.ncbi.nlm.nih.gov/pubmed/32525930
http://dx.doi.org/10.1371/journal.pone.0234395
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