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Local auxin competition explains fragmented differentiation patterns

Trajectories of cellular ontogeny are tightly controlled and often involve feedback-regulated molecular antagonism. For example, sieve element differentiation along developing protophloem cell files of Arabidopsis roots requires two antagonistic regulators of auxin efflux. Paradoxically, loss-of-fun...

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Autores principales: Moret, Bernard, Marhava, Petra, Aliaga Fandino, Ana Cecilia, Hardtke, Christian S., ten Tusscher, Kirsten H. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289883/
https://www.ncbi.nlm.nih.gov/pubmed/32528082
http://dx.doi.org/10.1038/s41467-020-16803-7
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author Moret, Bernard
Marhava, Petra
Aliaga Fandino, Ana Cecilia
Hardtke, Christian S.
ten Tusscher, Kirsten H. W.
author_facet Moret, Bernard
Marhava, Petra
Aliaga Fandino, Ana Cecilia
Hardtke, Christian S.
ten Tusscher, Kirsten H. W.
author_sort Moret, Bernard
collection PubMed
description Trajectories of cellular ontogeny are tightly controlled and often involve feedback-regulated molecular antagonism. For example, sieve element differentiation along developing protophloem cell files of Arabidopsis roots requires two antagonistic regulators of auxin efflux. Paradoxically, loss-of-function in either regulator triggers similar, seemingly stochastic differentiation failures of individual sieve element precursors. Here we show that these patterning defects are distinct and non-random. They can be explained by auxin-dependent bistability that emerges from competition for auxin between neighboring cells. This bistability depends on the presence of an auxin influx facilitator, and can be triggered by either flux enhancement or repression. Our results uncover a hitherto overlooked aspect of auxin uptake, and highlight the contributions of local auxin influx, efflux and biosynthesis to protophloem formation. Moreover, the combined experimental-modeling approach suggests that without auxin efflux homeostasis, auxin influx interferes with coordinated differentiation.
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spelling pubmed-72898832020-06-16 Local auxin competition explains fragmented differentiation patterns Moret, Bernard Marhava, Petra Aliaga Fandino, Ana Cecilia Hardtke, Christian S. ten Tusscher, Kirsten H. W. Nat Commun Article Trajectories of cellular ontogeny are tightly controlled and often involve feedback-regulated molecular antagonism. For example, sieve element differentiation along developing protophloem cell files of Arabidopsis roots requires two antagonistic regulators of auxin efflux. Paradoxically, loss-of-function in either regulator triggers similar, seemingly stochastic differentiation failures of individual sieve element precursors. Here we show that these patterning defects are distinct and non-random. They can be explained by auxin-dependent bistability that emerges from competition for auxin between neighboring cells. This bistability depends on the presence of an auxin influx facilitator, and can be triggered by either flux enhancement or repression. Our results uncover a hitherto overlooked aspect of auxin uptake, and highlight the contributions of local auxin influx, efflux and biosynthesis to protophloem formation. Moreover, the combined experimental-modeling approach suggests that without auxin efflux homeostasis, auxin influx interferes with coordinated differentiation. Nature Publishing Group UK 2020-06-11 /pmc/articles/PMC7289883/ /pubmed/32528082 http://dx.doi.org/10.1038/s41467-020-16803-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moret, Bernard
Marhava, Petra
Aliaga Fandino, Ana Cecilia
Hardtke, Christian S.
ten Tusscher, Kirsten H. W.
Local auxin competition explains fragmented differentiation patterns
title Local auxin competition explains fragmented differentiation patterns
title_full Local auxin competition explains fragmented differentiation patterns
title_fullStr Local auxin competition explains fragmented differentiation patterns
title_full_unstemmed Local auxin competition explains fragmented differentiation patterns
title_short Local auxin competition explains fragmented differentiation patterns
title_sort local auxin competition explains fragmented differentiation patterns
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289883/
https://www.ncbi.nlm.nih.gov/pubmed/32528082
http://dx.doi.org/10.1038/s41467-020-16803-7
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