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The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner

Wolbachia pipientis is an intracellular alphaproteobacterium that infects 40%–60% of insect species and is well known for host reproductive manipulations. Although Wolbachia are primarily maternally transmitted, evidence of horizontal transmission can be found in incongruent host–symbiont phylogenie...

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Autores principales: Bryant, Kaeli N., Newton, Irene L. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290356/
https://www.ncbi.nlm.nih.gov/pubmed/32384776
http://dx.doi.org/10.3390/insects11050284
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author Bryant, Kaeli N.
Newton, Irene L. G.
author_facet Bryant, Kaeli N.
Newton, Irene L. G.
author_sort Bryant, Kaeli N.
collection PubMed
description Wolbachia pipientis is an intracellular alphaproteobacterium that infects 40%–60% of insect species and is well known for host reproductive manipulations. Although Wolbachia are primarily maternally transmitted, evidence of horizontal transmission can be found in incongruent host–symbiont phylogenies and recent acquisitions of the same Wolbachia strain by distantly related species. Parasitoids and predator–prey interactions may indeed facilitate the transfer of Wolbachia between insect lineages, but it is likely that Wolbachia are acquired via introgression in many cases. Many hypotheses exist to explain Wolbachia prevalence and penetrance, such as nutritional supplementation, protection from parasites, protection from viruses, or direct reproductive parasitism. Using classical genetics, we show that Wolbachia increase recombination in infected lineages across two genomic intervals. This increase in recombination is titer-dependent as the wMelPop variant, which infects at higher load in Drosophila melanogaster, increases recombination 5% more than the wMel variant. In addition, we also show that Spiroplasma poulsonii, another bacterial intracellular symbiont of D. melanogaster, does not induce an increase in recombination. Our results suggest that Wolbachia infection specifically alters its host’s recombination landscape in a dose-dependent manner.
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spelling pubmed-72903562020-06-15 The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner Bryant, Kaeli N. Newton, Irene L. G. Insects Article Wolbachia pipientis is an intracellular alphaproteobacterium that infects 40%–60% of insect species and is well known for host reproductive manipulations. Although Wolbachia are primarily maternally transmitted, evidence of horizontal transmission can be found in incongruent host–symbiont phylogenies and recent acquisitions of the same Wolbachia strain by distantly related species. Parasitoids and predator–prey interactions may indeed facilitate the transfer of Wolbachia between insect lineages, but it is likely that Wolbachia are acquired via introgression in many cases. Many hypotheses exist to explain Wolbachia prevalence and penetrance, such as nutritional supplementation, protection from parasites, protection from viruses, or direct reproductive parasitism. Using classical genetics, we show that Wolbachia increase recombination in infected lineages across two genomic intervals. This increase in recombination is titer-dependent as the wMelPop variant, which infects at higher load in Drosophila melanogaster, increases recombination 5% more than the wMel variant. In addition, we also show that Spiroplasma poulsonii, another bacterial intracellular symbiont of D. melanogaster, does not induce an increase in recombination. Our results suggest that Wolbachia infection specifically alters its host’s recombination landscape in a dose-dependent manner. MDPI 2020-05-06 /pmc/articles/PMC7290356/ /pubmed/32384776 http://dx.doi.org/10.3390/insects11050284 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bryant, Kaeli N.
Newton, Irene L. G.
The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner
title The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner
title_full The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner
title_fullStr The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner
title_full_unstemmed The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner
title_short The Intracellular Symbiont Wolbachia pipientis Enhances Recombination in a Dose-Dependent Manner
title_sort intracellular symbiont wolbachia pipientis enhances recombination in a dose-dependent manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290356/
https://www.ncbi.nlm.nih.gov/pubmed/32384776
http://dx.doi.org/10.3390/insects11050284
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