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Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development
Branching morphogenesis is the basic developmental mode common to organs such as the lungs that undergo a process of ramification from a rudimentary tree. However, the precise molecular and cellular bases underlying the formation of branching organs are still unclear. As inactivation of fibroblast g...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290466/ https://www.ncbi.nlm.nih.gov/pubmed/32455591 http://dx.doi.org/10.3390/cells9051274 |
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author | Jones, Matthew R. Lingampally, Arun Wu, Jin Sedighi, Jamschid Ahmadvand, Negah Wilhelm, Jochen Vazquez-Armendariz, Ana Ivonne Herold, Susanne Chen, Chengshui Zhang, Jin-San Bellusci, Saverio Chao, Cho-Ming |
author_facet | Jones, Matthew R. Lingampally, Arun Wu, Jin Sedighi, Jamschid Ahmadvand, Negah Wilhelm, Jochen Vazquez-Armendariz, Ana Ivonne Herold, Susanne Chen, Chengshui Zhang, Jin-San Bellusci, Saverio Chao, Cho-Ming |
author_sort | Jones, Matthew R. |
collection | PubMed |
description | Branching morphogenesis is the basic developmental mode common to organs such as the lungs that undergo a process of ramification from a rudimentary tree. However, the precise molecular and cellular bases underlying the formation of branching organs are still unclear. As inactivation of fibroblast growth factor receptor 2b (Fgfr2b) signaling during early development leads to lung agenesis, thereby preventing the analysis of this pathway at later developmental stages, we used transgenic mice to induce expression of a soluble form of Fgfr2b to inactivate Fgfr2b ligands at embryonic day (E) 14.5, corresponding to the mid-pseudoglandular stage of lung development. We identified an Fgfr2b signaling signature comprised of 46 genes enriched in the epithelium, some of which were common to, but most of them distinct from, the previously identified Fgfr2b signaling signature at E12.5. Our results indicate that Fgfr2b signaling at E14.5 controls mostly proliferation and alveolar type 2 cell (AT2) differentiation. In addition, inhibition of Fgfr2b signaling at E14.5 leads to morphological and cellular impairment at E18.5, with defective alveolar lineage formation. Further studies will have to be conducted to elucidate the role of Fgfr2b signaling at successive stages (canalicular/saccular/alveolar) of lung development as well as during homeostasis and regeneration and repair after injury. |
format | Online Article Text |
id | pubmed-7290466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72904662020-06-15 Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development Jones, Matthew R. Lingampally, Arun Wu, Jin Sedighi, Jamschid Ahmadvand, Negah Wilhelm, Jochen Vazquez-Armendariz, Ana Ivonne Herold, Susanne Chen, Chengshui Zhang, Jin-San Bellusci, Saverio Chao, Cho-Ming Cells Article Branching morphogenesis is the basic developmental mode common to organs such as the lungs that undergo a process of ramification from a rudimentary tree. However, the precise molecular and cellular bases underlying the formation of branching organs are still unclear. As inactivation of fibroblast growth factor receptor 2b (Fgfr2b) signaling during early development leads to lung agenesis, thereby preventing the analysis of this pathway at later developmental stages, we used transgenic mice to induce expression of a soluble form of Fgfr2b to inactivate Fgfr2b ligands at embryonic day (E) 14.5, corresponding to the mid-pseudoglandular stage of lung development. We identified an Fgfr2b signaling signature comprised of 46 genes enriched in the epithelium, some of which were common to, but most of them distinct from, the previously identified Fgfr2b signaling signature at E12.5. Our results indicate that Fgfr2b signaling at E14.5 controls mostly proliferation and alveolar type 2 cell (AT2) differentiation. In addition, inhibition of Fgfr2b signaling at E14.5 leads to morphological and cellular impairment at E18.5, with defective alveolar lineage formation. Further studies will have to be conducted to elucidate the role of Fgfr2b signaling at successive stages (canalicular/saccular/alveolar) of lung development as well as during homeostasis and regeneration and repair after injury. MDPI 2020-05-21 /pmc/articles/PMC7290466/ /pubmed/32455591 http://dx.doi.org/10.3390/cells9051274 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jones, Matthew R. Lingampally, Arun Wu, Jin Sedighi, Jamschid Ahmadvand, Negah Wilhelm, Jochen Vazquez-Armendariz, Ana Ivonne Herold, Susanne Chen, Chengshui Zhang, Jin-San Bellusci, Saverio Chao, Cho-Ming Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development |
title | Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development |
title_full | Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development |
title_fullStr | Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development |
title_full_unstemmed | Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development |
title_short | Evidence for Overlapping and Distinct Biological Activities and Transcriptional Targets Triggered by Fibroblast Growth Factor Receptor 2b Signaling between Mid- and Early Pseudoglandular Stages of Mouse Lung Development |
title_sort | evidence for overlapping and distinct biological activities and transcriptional targets triggered by fibroblast growth factor receptor 2b signaling between mid- and early pseudoglandular stages of mouse lung development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290466/ https://www.ncbi.nlm.nih.gov/pubmed/32455591 http://dx.doi.org/10.3390/cells9051274 |
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