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Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions

Different batches of biomass/feed quality maize contaminated by aflatoxins were processed at the industrial scale (a continuous process and separate discontinuous steps) to evaluate the effect of different cleaning solutions on toxin reduction. The investigated cleaning solutions included: (i) mecha...

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Autores principales: Pascale, Michelangelo, Logrieco, Antonio F., Graeber, Matthias, Hirschberger, Marina, Reichel, Mareike, Lippolis, Vincenzo, De Girolamo, Annalisa, Lattanzio, Veronica M. T., Slettengren, Katarina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290569/
https://www.ncbi.nlm.nih.gov/pubmed/32429556
http://dx.doi.org/10.3390/toxins12050331
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author Pascale, Michelangelo
Logrieco, Antonio F.
Graeber, Matthias
Hirschberger, Marina
Reichel, Mareike
Lippolis, Vincenzo
De Girolamo, Annalisa
Lattanzio, Veronica M. T.
Slettengren, Katarina
author_facet Pascale, Michelangelo
Logrieco, Antonio F.
Graeber, Matthias
Hirschberger, Marina
Reichel, Mareike
Lippolis, Vincenzo
De Girolamo, Annalisa
Lattanzio, Veronica M. T.
Slettengren, Katarina
author_sort Pascale, Michelangelo
collection PubMed
description Different batches of biomass/feed quality maize contaminated by aflatoxins were processed at the industrial scale (a continuous process and separate discontinuous steps) to evaluate the effect of different cleaning solutions on toxin reduction. The investigated cleaning solutions included: (i) mechanical size separation of coarse, small and broken kernels, (ii) removal of dust/fine particles through an aspiration channel, (iii) separation of kernels based on gravity and (iv) optical sorting of spatial and spectral kernel defects. Depending on the sampled fraction, dynamic or static sampling was performed according to the Commission Regulation No. 401/2006 along the entire cleaning process lines. Aflatoxin analyses of the water–slurry aggregate samples were performed according to the AOAC Official Method No. 2005.008 based on high-performance liquid chromatography and immunoaffinity column cleanup of the extracts. A significant reduction in aflatoxin content in the cleaned products, ranging from 65% to 84% with respect to the uncleaned products, was observed when continuous cleaning lines were used. Additionally, an overall aflatoxin reduction from 55% to 94% was obtained by combining results from separate cleaning steps. High levels of aflatoxins (up to 490 µg/kg) were found in the rejected fractions, with the highest levels in dust and in the rejected fractions from the aspirator and optical sorting. This study shows that a cleaning line combining both mechanical and optical sorting technologies provides an efficient solution for reducing aflatoxin contamination in maize.
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spelling pubmed-72905692020-06-17 Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions Pascale, Michelangelo Logrieco, Antonio F. Graeber, Matthias Hirschberger, Marina Reichel, Mareike Lippolis, Vincenzo De Girolamo, Annalisa Lattanzio, Veronica M. T. Slettengren, Katarina Toxins (Basel) Article Different batches of biomass/feed quality maize contaminated by aflatoxins were processed at the industrial scale (a continuous process and separate discontinuous steps) to evaluate the effect of different cleaning solutions on toxin reduction. The investigated cleaning solutions included: (i) mechanical size separation of coarse, small and broken kernels, (ii) removal of dust/fine particles through an aspiration channel, (iii) separation of kernels based on gravity and (iv) optical sorting of spatial and spectral kernel defects. Depending on the sampled fraction, dynamic or static sampling was performed according to the Commission Regulation No. 401/2006 along the entire cleaning process lines. Aflatoxin analyses of the water–slurry aggregate samples were performed according to the AOAC Official Method No. 2005.008 based on high-performance liquid chromatography and immunoaffinity column cleanup of the extracts. A significant reduction in aflatoxin content in the cleaned products, ranging from 65% to 84% with respect to the uncleaned products, was observed when continuous cleaning lines were used. Additionally, an overall aflatoxin reduction from 55% to 94% was obtained by combining results from separate cleaning steps. High levels of aflatoxins (up to 490 µg/kg) were found in the rejected fractions, with the highest levels in dust and in the rejected fractions from the aspirator and optical sorting. This study shows that a cleaning line combining both mechanical and optical sorting technologies provides an efficient solution for reducing aflatoxin contamination in maize. MDPI 2020-05-17 /pmc/articles/PMC7290569/ /pubmed/32429556 http://dx.doi.org/10.3390/toxins12050331 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pascale, Michelangelo
Logrieco, Antonio F.
Graeber, Matthias
Hirschberger, Marina
Reichel, Mareike
Lippolis, Vincenzo
De Girolamo, Annalisa
Lattanzio, Veronica M. T.
Slettengren, Katarina
Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions
title Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions
title_full Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions
title_fullStr Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions
title_full_unstemmed Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions
title_short Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions
title_sort aflatoxin reduction in maize by industrial-scale cleaning solutions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290569/
https://www.ncbi.nlm.nih.gov/pubmed/32429556
http://dx.doi.org/10.3390/toxins12050331
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