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Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL
Background: T lymphocyte collection through leukapheresis is an essential step for chimeric antigen receptor T (CAR-T) cell therapy. Timing of apheresis is challenging in heavily pretreated patients who suffer from rapid progressive disease and receive T cell impairing medication. Methods: A total o...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290830/ https://www.ncbi.nlm.nih.gov/pubmed/32429189 http://dx.doi.org/10.3390/cells9051225 |
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author | Korell, Felix Laier, Sascha Sauer, Sandra Veelken, Kaya Hennemann, Hannah Schubert, Maria-Luisa Sauer, Tim Pavel, Petra Mueller-Tidow, Carsten Dreger, Peter Schmitt, Michael Schmitt, Anita |
author_facet | Korell, Felix Laier, Sascha Sauer, Sandra Veelken, Kaya Hennemann, Hannah Schubert, Maria-Luisa Sauer, Tim Pavel, Petra Mueller-Tidow, Carsten Dreger, Peter Schmitt, Michael Schmitt, Anita |
author_sort | Korell, Felix |
collection | PubMed |
description | Background: T lymphocyte collection through leukapheresis is an essential step for chimeric antigen receptor T (CAR-T) cell therapy. Timing of apheresis is challenging in heavily pretreated patients who suffer from rapid progressive disease and receive T cell impairing medication. Methods: A total of 75 unstimulated leukaphereses were analyzed including 45 aphereses in patients and 30 in healthy donors. Thereof, 41 adult patients with Non-Hodgkin’s lymphoma (85%) or acute lymphoblastic leukemia (15%) underwent leukapheresis for CAR-T cell production. Results: Sufficient lymphocytes were harvested from all patients even from those with low peripheral lymphocyte counts of 0.18/nL. Only four patients required a second leukapheresis session. Leukapheresis products contained a median of 98 × 10(8) (9 - 341 × 10(8)) total nucleated cells (TNC) with 38 × 10(8) (4 - 232 × 10(8)) CD3+ T cells. Leukapheresis products from healthy donors as well as from patients in complete remission were characterized by high TNC and CD3+ T lymphocyte counts. CAR-T cell products could be manufactured for all but one patient. Conclusions: Sufficient yield of lymphocytes for CAR-T cell production is feasible also for patients with low peripheral blood counts. Up to 12–15 L blood volume should be processed in patients with absolute lymphocyte counts ≤ 1.0/nL. |
format | Online Article Text |
id | pubmed-7290830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72908302020-06-17 Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL Korell, Felix Laier, Sascha Sauer, Sandra Veelken, Kaya Hennemann, Hannah Schubert, Maria-Luisa Sauer, Tim Pavel, Petra Mueller-Tidow, Carsten Dreger, Peter Schmitt, Michael Schmitt, Anita Cells Article Background: T lymphocyte collection through leukapheresis is an essential step for chimeric antigen receptor T (CAR-T) cell therapy. Timing of apheresis is challenging in heavily pretreated patients who suffer from rapid progressive disease and receive T cell impairing medication. Methods: A total of 75 unstimulated leukaphereses were analyzed including 45 aphereses in patients and 30 in healthy donors. Thereof, 41 adult patients with Non-Hodgkin’s lymphoma (85%) or acute lymphoblastic leukemia (15%) underwent leukapheresis for CAR-T cell production. Results: Sufficient lymphocytes were harvested from all patients even from those with low peripheral lymphocyte counts of 0.18/nL. Only four patients required a second leukapheresis session. Leukapheresis products contained a median of 98 × 10(8) (9 - 341 × 10(8)) total nucleated cells (TNC) with 38 × 10(8) (4 - 232 × 10(8)) CD3+ T cells. Leukapheresis products from healthy donors as well as from patients in complete remission were characterized by high TNC and CD3+ T lymphocyte counts. CAR-T cell products could be manufactured for all but one patient. Conclusions: Sufficient yield of lymphocytes for CAR-T cell production is feasible also for patients with low peripheral blood counts. Up to 12–15 L blood volume should be processed in patients with absolute lymphocyte counts ≤ 1.0/nL. MDPI 2020-05-15 /pmc/articles/PMC7290830/ /pubmed/32429189 http://dx.doi.org/10.3390/cells9051225 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Korell, Felix Laier, Sascha Sauer, Sandra Veelken, Kaya Hennemann, Hannah Schubert, Maria-Luisa Sauer, Tim Pavel, Petra Mueller-Tidow, Carsten Dreger, Peter Schmitt, Michael Schmitt, Anita Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL |
title | Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL |
title_full | Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL |
title_fullStr | Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL |
title_full_unstemmed | Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL |
title_short | Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL |
title_sort | current challenges in providing good leukapheresis products for manufacturing of car-t cells for patients with relapsed/refractory nhl or all |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290830/ https://www.ncbi.nlm.nih.gov/pubmed/32429189 http://dx.doi.org/10.3390/cells9051225 |
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