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Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats

Exposure to lead (Pb) causes multiorgan dysfunction including reproductive impairments. Here, we examined the protective effects of coenzyme Q10 (CoQ10) administration on testicular injury induced by lead acetate (PbAc) exposure in rats. This study employed four experimental groups (n = 7) that unde...

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Autores principales: El-khadragy, Manal, Al-Megrin, Wafa A., AlSadhan, Norah A., Metwally, Dina M., El-Hennamy, Rehab E., Salem, Fatma Elzahraa H., Kassab, Rami B., Abdel Moneim, Ahmed E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290879/
https://www.ncbi.nlm.nih.gov/pubmed/32566085
http://dx.doi.org/10.1155/2020/4981386
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author El-khadragy, Manal
Al-Megrin, Wafa A.
AlSadhan, Norah A.
Metwally, Dina M.
El-Hennamy, Rehab E.
Salem, Fatma Elzahraa H.
Kassab, Rami B.
Abdel Moneim, Ahmed E.
author_facet El-khadragy, Manal
Al-Megrin, Wafa A.
AlSadhan, Norah A.
Metwally, Dina M.
El-Hennamy, Rehab E.
Salem, Fatma Elzahraa H.
Kassab, Rami B.
Abdel Moneim, Ahmed E.
author_sort El-khadragy, Manal
collection PubMed
description Exposure to lead (Pb) causes multiorgan dysfunction including reproductive impairments. Here, we examined the protective effects of coenzyme Q10 (CoQ10) administration on testicular injury induced by lead acetate (PbAc) exposure in rats. This study employed four experimental groups (n = 7) that underwent seven days of treatment as follows: control group intraperitoneally (i.p.) treated with 0.1 ml of 0.9% NaCl containing 1% Tween 80 (v : v), CoQ10 group that was i.p. injected with 10 mg/kg CoQ10, PbAc group that was i.p. treated with PbAc (20 mg/kg), and PbAc+CoQ10 group that was i.p. injected with CoQ10 2 h after PbAc. PbAc injection resulted in increasing residual Pb levels in the testis and reducing testosterone, luteinizing hormone, and follicle-stimulating hormone levels. Additionally, PbAc exposure resulted in significant oxidative damage to the tissues on the testes. PbAc raised the levels of prooxidants (malondialdehyde and nitric oxide) and reduced the amount of endogenous antioxidative proteins (glutathione and its derivative enzymes, catalase, and superoxide dismutase) available in the cell. Moreover, PbAc induced the inflammatory response as evidenced by the upregulation of inflammatory mediators (tumor necrosis factor-alpha and interleukin-1 beta). Further, PbAc treatment induced apoptosis in the testicular cells, as indicated by an increase in Bax and caspase 3 expression, and reduced Bcl2 expression. CoQ10 supplementation improved testicular function by inhibiting Pb accumulation, oxidative stress, inflammation, cell death, and histopathological changes following PbAc exposure. Our findings suggest that CoQ10 can act as a natural therapeutic agent to protect against the reproductive impairments associated with PbAc exposure.
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spelling pubmed-72908792020-06-19 Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats El-khadragy, Manal Al-Megrin, Wafa A. AlSadhan, Norah A. Metwally, Dina M. El-Hennamy, Rehab E. Salem, Fatma Elzahraa H. Kassab, Rami B. Abdel Moneim, Ahmed E. Oxid Med Cell Longev Research Article Exposure to lead (Pb) causes multiorgan dysfunction including reproductive impairments. Here, we examined the protective effects of coenzyme Q10 (CoQ10) administration on testicular injury induced by lead acetate (PbAc) exposure in rats. This study employed four experimental groups (n = 7) that underwent seven days of treatment as follows: control group intraperitoneally (i.p.) treated with 0.1 ml of 0.9% NaCl containing 1% Tween 80 (v : v), CoQ10 group that was i.p. injected with 10 mg/kg CoQ10, PbAc group that was i.p. treated with PbAc (20 mg/kg), and PbAc+CoQ10 group that was i.p. injected with CoQ10 2 h after PbAc. PbAc injection resulted in increasing residual Pb levels in the testis and reducing testosterone, luteinizing hormone, and follicle-stimulating hormone levels. Additionally, PbAc exposure resulted in significant oxidative damage to the tissues on the testes. PbAc raised the levels of prooxidants (malondialdehyde and nitric oxide) and reduced the amount of endogenous antioxidative proteins (glutathione and its derivative enzymes, catalase, and superoxide dismutase) available in the cell. Moreover, PbAc induced the inflammatory response as evidenced by the upregulation of inflammatory mediators (tumor necrosis factor-alpha and interleukin-1 beta). Further, PbAc treatment induced apoptosis in the testicular cells, as indicated by an increase in Bax and caspase 3 expression, and reduced Bcl2 expression. CoQ10 supplementation improved testicular function by inhibiting Pb accumulation, oxidative stress, inflammation, cell death, and histopathological changes following PbAc exposure. Our findings suggest that CoQ10 can act as a natural therapeutic agent to protect against the reproductive impairments associated with PbAc exposure. Hindawi 2020-06-02 /pmc/articles/PMC7290879/ /pubmed/32566085 http://dx.doi.org/10.1155/2020/4981386 Text en Copyright © 2020 Manal El-khadragy et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
El-khadragy, Manal
Al-Megrin, Wafa A.
AlSadhan, Norah A.
Metwally, Dina M.
El-Hennamy, Rehab E.
Salem, Fatma Elzahraa H.
Kassab, Rami B.
Abdel Moneim, Ahmed E.
Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats
title Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats
title_full Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats
title_fullStr Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats
title_full_unstemmed Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats
title_short Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats
title_sort impact of coenzyme q10 administration on lead acetate-induced testicular damage in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290879/
https://www.ncbi.nlm.nih.gov/pubmed/32566085
http://dx.doi.org/10.1155/2020/4981386
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