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Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans

Mobile DNA elements play a significant evolutionary role by promoting genome plasticity. Insertion sequences are the smallest prokaryotic transposable elements. They are highly diverse elements, and the ability to accurately identify, annotate, and infer the full genomic impact of insertion sequence...

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Detalles Bibliográficos
Autores principales: Sadler, Michael, R. Mormile, Melanie, L. Frank, Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290912/
https://www.ncbi.nlm.nih.gov/pubmed/32365520
http://dx.doi.org/10.3390/genes11050484
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author Sadler, Michael
R. Mormile, Melanie
L. Frank, Ronald
author_facet Sadler, Michael
R. Mormile, Melanie
L. Frank, Ronald
author_sort Sadler, Michael
collection PubMed
description Mobile DNA elements play a significant evolutionary role by promoting genome plasticity. Insertion sequences are the smallest prokaryotic transposable elements. They are highly diverse elements, and the ability to accurately identify, annotate, and infer the full genomic impact of insertion sequences is lacking. Halanaerobium hydrogeniformans is a haloalkaliphilic bacterium with an abnormally high number of insertion sequences. One family, IS200/IS605, showed several interesting features distinct from other elements in this genome. Twenty-three loci harbor elements of this family in varying stages of decay, from nearly intact to an ends-only sequence. The loci were characterized with respect to two divergent open reading frames (ORF), tnpA and tnpB, and left and right ends of the elements. The tnpB ORF contains two nearly identical insert sequences that suggest recombination between tnpB ORF is occurring. From these results, insertion sequence activity can be inferred, including transposition capability and element interaction.
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spelling pubmed-72909122020-06-17 Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans Sadler, Michael R. Mormile, Melanie L. Frank, Ronald Genes (Basel) Article Mobile DNA elements play a significant evolutionary role by promoting genome plasticity. Insertion sequences are the smallest prokaryotic transposable elements. They are highly diverse elements, and the ability to accurately identify, annotate, and infer the full genomic impact of insertion sequences is lacking. Halanaerobium hydrogeniformans is a haloalkaliphilic bacterium with an abnormally high number of insertion sequences. One family, IS200/IS605, showed several interesting features distinct from other elements in this genome. Twenty-three loci harbor elements of this family in varying stages of decay, from nearly intact to an ends-only sequence. The loci were characterized with respect to two divergent open reading frames (ORF), tnpA and tnpB, and left and right ends of the elements. The tnpB ORF contains two nearly identical insert sequences that suggest recombination between tnpB ORF is occurring. From these results, insertion sequence activity can be inferred, including transposition capability and element interaction. MDPI 2020-04-29 /pmc/articles/PMC7290912/ /pubmed/32365520 http://dx.doi.org/10.3390/genes11050484 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sadler, Michael
R. Mormile, Melanie
L. Frank, Ronald
Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans
title Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans
title_full Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans
title_fullStr Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans
title_full_unstemmed Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans
title_short Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans
title_sort characterization of the is200/is605 insertion sequence family in halanaerobium hydrogeniformans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290912/
https://www.ncbi.nlm.nih.gov/pubmed/32365520
http://dx.doi.org/10.3390/genes11050484
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