Cargando…
Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection
Food and crop contaminations with mycotoxins are a severe health risk for consumers and cause high economic losses worldwide. Currently, different chromatographic- and immuno-based methods are used to detect mycotoxins within different sample matrices. There is a need for novel, highly sensitive det...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290995/ https://www.ncbi.nlm.nih.gov/pubmed/32443933 http://dx.doi.org/10.3390/toxins12050337 |
_version_ | 1783545806613118976 |
---|---|
author | Pietschmann, Jan Spiegel, Holger Krause, Hans-Joachim Schillberg, Stefan Schröper, Florian |
author_facet | Pietschmann, Jan Spiegel, Holger Krause, Hans-Joachim Schillberg, Stefan Schröper, Florian |
author_sort | Pietschmann, Jan |
collection | PubMed |
description | Food and crop contaminations with mycotoxins are a severe health risk for consumers and cause high economic losses worldwide. Currently, different chromatographic- and immuno-based methods are used to detect mycotoxins within different sample matrices. There is a need for novel, highly sensitive detection technologies that avoid time-consuming procedures and expensive laboratory equipment but still provide sufficient sensitivity to achieve the mandated detection limit for mycotoxin content. Here we describe a novel, highly sensitive, and portable aflatoxin B1 detection approach using competitive magnetic immunodetection (cMID). As a reference method, a competitive ELISA optimized by checkerboard titration was established. For the novel cMID procedure, immunofiltration columns, coated with aflatoxin B1-BSA conjugate were used for competitive enrichment of biotinylated aflatoxin B1-specific antibodies. Subsequently, magnetic particles functionalized with streptavidin can be applied to magnetically label retained antibodies. By means of frequency mixing technology, particles were detected and quantified corresponding to the aflatoxin content in the sample. After the optimization of assay conditions, we successfully demonstrated the new competitive magnetic detection approach with a comparable detection limit of 1.1 ng aflatoxin B1 per mL sample to the cELISA reference method. Our results indicate that the cMID is a promising method reducing the risks of processing contaminated commodities. |
format | Online Article Text |
id | pubmed-7290995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72909952020-06-17 Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection Pietschmann, Jan Spiegel, Holger Krause, Hans-Joachim Schillberg, Stefan Schröper, Florian Toxins (Basel) Article Food and crop contaminations with mycotoxins are a severe health risk for consumers and cause high economic losses worldwide. Currently, different chromatographic- and immuno-based methods are used to detect mycotoxins within different sample matrices. There is a need for novel, highly sensitive detection technologies that avoid time-consuming procedures and expensive laboratory equipment but still provide sufficient sensitivity to achieve the mandated detection limit for mycotoxin content. Here we describe a novel, highly sensitive, and portable aflatoxin B1 detection approach using competitive magnetic immunodetection (cMID). As a reference method, a competitive ELISA optimized by checkerboard titration was established. For the novel cMID procedure, immunofiltration columns, coated with aflatoxin B1-BSA conjugate were used for competitive enrichment of biotinylated aflatoxin B1-specific antibodies. Subsequently, magnetic particles functionalized with streptavidin can be applied to magnetically label retained antibodies. By means of frequency mixing technology, particles were detected and quantified corresponding to the aflatoxin content in the sample. After the optimization of assay conditions, we successfully demonstrated the new competitive magnetic detection approach with a comparable detection limit of 1.1 ng aflatoxin B1 per mL sample to the cELISA reference method. Our results indicate that the cMID is a promising method reducing the risks of processing contaminated commodities. MDPI 2020-05-20 /pmc/articles/PMC7290995/ /pubmed/32443933 http://dx.doi.org/10.3390/toxins12050337 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pietschmann, Jan Spiegel, Holger Krause, Hans-Joachim Schillberg, Stefan Schröper, Florian Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection |
title | Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection |
title_full | Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection |
title_fullStr | Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection |
title_full_unstemmed | Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection |
title_short | Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection |
title_sort | sensitive aflatoxin b1 detection using nanoparticle-based competitive magnetic immunodetection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290995/ https://www.ncbi.nlm.nih.gov/pubmed/32443933 http://dx.doi.org/10.3390/toxins12050337 |
work_keys_str_mv | AT pietschmannjan sensitiveaflatoxinb1detectionusingnanoparticlebasedcompetitivemagneticimmunodetection AT spiegelholger sensitiveaflatoxinb1detectionusingnanoparticlebasedcompetitivemagneticimmunodetection AT krausehansjoachim sensitiveaflatoxinb1detectionusingnanoparticlebasedcompetitivemagneticimmunodetection AT schillbergstefan sensitiveaflatoxinb1detectionusingnanoparticlebasedcompetitivemagneticimmunodetection AT schroperflorian sensitiveaflatoxinb1detectionusingnanoparticlebasedcompetitivemagneticimmunodetection |