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Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors

This study was performed to evaluate the effects of 15-month anti-tumor necrosis factor α (anti-TNF-α) therapy on the aggrecan turnover of female rheumatoid arthritis (RA) patients. Serum was obtained from healthy subjects and female RA patients treated with TNF-α inhibitors (TNFαI) in combination w...

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Autores principales: Szeremeta, Anna, Jura-Półtorak, Agnieszka, Zoń-Giebel, Aleksandra, Kopeć-Mędrek, Magdalena, Kucharz, Eugeniusz Józef, Olczyk, Krystyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290997/
https://www.ncbi.nlm.nih.gov/pubmed/32392807
http://dx.doi.org/10.3390/jcm9051377
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author Szeremeta, Anna
Jura-Półtorak, Agnieszka
Zoń-Giebel, Aleksandra
Kopeć-Mędrek, Magdalena
Kucharz, Eugeniusz Józef
Olczyk, Krystyna
author_facet Szeremeta, Anna
Jura-Półtorak, Agnieszka
Zoń-Giebel, Aleksandra
Kopeć-Mędrek, Magdalena
Kucharz, Eugeniusz Józef
Olczyk, Krystyna
author_sort Szeremeta, Anna
collection PubMed
description This study was performed to evaluate the effects of 15-month anti-tumor necrosis factor α (anti-TNF-α) therapy on the aggrecan turnover of female rheumatoid arthritis (RA) patients. Serum was obtained from healthy subjects and female RA patients treated with TNF-α inhibitors (TNFαI) in combination with methotrexate. We measured serum levels of aggrecan chondroitin sulfate 846 epitope (CS846), aggrecan fragments (AGC), disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4) and 5 (ADAMTS-5), as well as their natural inhibitor, known as tissue inhibitor of matrix metalloproteinase-3 (TIMP-3), using immunoassay methods. Serum levels of CS846, AGC, ADAMTS-4, ADAMTS-5 and TIMP-3 were higher in female patients with RA before the treatment in comparison to healthy subjects. Ratio of ADAMTS-5 to TIMP-3 was significantly higher in RA women than in controls, whereas ADAMTS-4/TIMP-3 ratio did not differ from that in controls. During the anti-TNF-α therapy, the serum levels of 846 epitope increased, whereas levels of AGC decreased in female RA patients. Furthermore, 15 months of treatment with TNFαI downregulated serum levels of both ADAMTS, without any effect on TIMP-3 levels. These changes were accompanied by significantly reduced ratios of ADAMTS to TIMP-3. According to our results, anti-TNF-α therapy has a beneficial impact on aggrecan remodeling during RA.
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spelling pubmed-72909972020-06-17 Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors Szeremeta, Anna Jura-Półtorak, Agnieszka Zoń-Giebel, Aleksandra Kopeć-Mędrek, Magdalena Kucharz, Eugeniusz Józef Olczyk, Krystyna J Clin Med Article This study was performed to evaluate the effects of 15-month anti-tumor necrosis factor α (anti-TNF-α) therapy on the aggrecan turnover of female rheumatoid arthritis (RA) patients. Serum was obtained from healthy subjects and female RA patients treated with TNF-α inhibitors (TNFαI) in combination with methotrexate. We measured serum levels of aggrecan chondroitin sulfate 846 epitope (CS846), aggrecan fragments (AGC), disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4) and 5 (ADAMTS-5), as well as their natural inhibitor, known as tissue inhibitor of matrix metalloproteinase-3 (TIMP-3), using immunoassay methods. Serum levels of CS846, AGC, ADAMTS-4, ADAMTS-5 and TIMP-3 were higher in female patients with RA before the treatment in comparison to healthy subjects. Ratio of ADAMTS-5 to TIMP-3 was significantly higher in RA women than in controls, whereas ADAMTS-4/TIMP-3 ratio did not differ from that in controls. During the anti-TNF-α therapy, the serum levels of 846 epitope increased, whereas levels of AGC decreased in female RA patients. Furthermore, 15 months of treatment with TNFαI downregulated serum levels of both ADAMTS, without any effect on TIMP-3 levels. These changes were accompanied by significantly reduced ratios of ADAMTS to TIMP-3. According to our results, anti-TNF-α therapy has a beneficial impact on aggrecan remodeling during RA. MDPI 2020-05-07 /pmc/articles/PMC7290997/ /pubmed/32392807 http://dx.doi.org/10.3390/jcm9051377 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szeremeta, Anna
Jura-Półtorak, Agnieszka
Zoń-Giebel, Aleksandra
Kopeć-Mędrek, Magdalena
Kucharz, Eugeniusz Józef
Olczyk, Krystyna
Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors
title Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors
title_full Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors
title_fullStr Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors
title_full_unstemmed Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors
title_short Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors
title_sort aggrecan turnover in women with rheumatoid arthritis treated with tnf-α inhibitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290997/
https://www.ncbi.nlm.nih.gov/pubmed/32392807
http://dx.doi.org/10.3390/jcm9051377
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