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Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses
In this study, we investigated the functional role of isoprenylcysteine carboxyl methyltransferase (ICMT) and its methylatable substrate Ras in Toll-like receptor (TLR)-activated macrophages and in mouse inflammatory disease conditions. ICMT and RAS expressions were strongly increased in macrophages...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291029/ https://www.ncbi.nlm.nih.gov/pubmed/32422978 http://dx.doi.org/10.3390/cells9051216 |
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author | Yang, Woo Seok Kim, Han Gyung Kim, Eunji Han, Sang Yun Aziz, Nur Yi, Young-Su Kim, Sunggyu Lee, Yunmi Yoo, Byong Chul Han, Jeung-Whan Parameswaran, Narayanan Kim, Ji Hye Cho, Jae Youl |
author_facet | Yang, Woo Seok Kim, Han Gyung Kim, Eunji Han, Sang Yun Aziz, Nur Yi, Young-Su Kim, Sunggyu Lee, Yunmi Yoo, Byong Chul Han, Jeung-Whan Parameswaran, Narayanan Kim, Ji Hye Cho, Jae Youl |
author_sort | Yang, Woo Seok |
collection | PubMed |
description | In this study, we investigated the functional role of isoprenylcysteine carboxyl methyltransferase (ICMT) and its methylatable substrate Ras in Toll-like receptor (TLR)-activated macrophages and in mouse inflammatory disease conditions. ICMT and RAS expressions were strongly increased in macrophages under the activation conditions of TLRs by lipopolysaccharide (LPS, a TLR4 ligand), pam3CSK (TLR2), or poly(I:C) (TLR3) and in the colons, stomachs, and livers of mice with colitis, gastritis, and hepatitis. The inhibition and activation of ICMT and Ras through genetic and pharmacological approaches significantly affected the activation of interleukin-1 receptor-associated kinase (IRAK)s, tumor necrosis factor receptor associated factor 6 (TRAF6), transforming growth factor-β-activated kinase 1 (TAK1), mitogen-activated protein kinase (MAPK), and MAPK kinases (MAPKKs); translocation of the AP-1 family; and the expressions of inflammation-related genes that depend on both MyD88 and TRIF. Interestingly, the Ras/ICMT-mediated inflammatory reaction critically depends on the TIR domains of myeloid differentiation primary response 88 (MyD88) and TIR-domain-containing adapter-inducing interferon-β (TRIF). Taken together, these results suggest that ICMT and its methylated Ras play important roles in the regulation of inflammatory responses through cooperation with the TIR domain of adaptor molecules. |
format | Online Article Text |
id | pubmed-7291029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72910292020-06-17 Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses Yang, Woo Seok Kim, Han Gyung Kim, Eunji Han, Sang Yun Aziz, Nur Yi, Young-Su Kim, Sunggyu Lee, Yunmi Yoo, Byong Chul Han, Jeung-Whan Parameswaran, Narayanan Kim, Ji Hye Cho, Jae Youl Cells Article In this study, we investigated the functional role of isoprenylcysteine carboxyl methyltransferase (ICMT) and its methylatable substrate Ras in Toll-like receptor (TLR)-activated macrophages and in mouse inflammatory disease conditions. ICMT and RAS expressions were strongly increased in macrophages under the activation conditions of TLRs by lipopolysaccharide (LPS, a TLR4 ligand), pam3CSK (TLR2), or poly(I:C) (TLR3) and in the colons, stomachs, and livers of mice with colitis, gastritis, and hepatitis. The inhibition and activation of ICMT and Ras through genetic and pharmacological approaches significantly affected the activation of interleukin-1 receptor-associated kinase (IRAK)s, tumor necrosis factor receptor associated factor 6 (TRAF6), transforming growth factor-β-activated kinase 1 (TAK1), mitogen-activated protein kinase (MAPK), and MAPK kinases (MAPKKs); translocation of the AP-1 family; and the expressions of inflammation-related genes that depend on both MyD88 and TRIF. Interestingly, the Ras/ICMT-mediated inflammatory reaction critically depends on the TIR domains of myeloid differentiation primary response 88 (MyD88) and TIR-domain-containing adapter-inducing interferon-β (TRIF). Taken together, these results suggest that ICMT and its methylated Ras play important roles in the regulation of inflammatory responses through cooperation with the TIR domain of adaptor molecules. MDPI 2020-05-14 /pmc/articles/PMC7291029/ /pubmed/32422978 http://dx.doi.org/10.3390/cells9051216 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Woo Seok Kim, Han Gyung Kim, Eunji Han, Sang Yun Aziz, Nur Yi, Young-Su Kim, Sunggyu Lee, Yunmi Yoo, Byong Chul Han, Jeung-Whan Parameswaran, Narayanan Kim, Ji Hye Cho, Jae Youl Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses |
title | Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses |
title_full | Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses |
title_fullStr | Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses |
title_full_unstemmed | Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses |
title_short | Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses |
title_sort | isoprenylcysteine carboxyl methyltransferase and its substrate ras are critical players regulating tlr-mediated inflammatory responses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291029/ https://www.ncbi.nlm.nih.gov/pubmed/32422978 http://dx.doi.org/10.3390/cells9051216 |
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