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Glycoprotein Profile Assessed by (1)H-NMR as a Global Inflammation Marker in Patients with HIV Infection. A Prospective Study
Plasma glycoproteins are a composite biomarker of inflammation and can be detected by (1)H-NMR. The aim of this study was to prospectively appraise the clinical value of plasma glycoproteins assessed by (1)H-NMR in people living with HIV (PLWH). A total of 221 patients with HIV infection were recrui...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291035/ https://www.ncbi.nlm.nih.gov/pubmed/32375373 http://dx.doi.org/10.3390/jcm9051344 |
Sumario: | Plasma glycoproteins are a composite biomarker of inflammation and can be detected by (1)H-NMR. The aim of this study was to prospectively appraise the clinical value of plasma glycoproteins assessed by (1)H-NMR in people living with HIV (PLWH). A total of 221 patients with HIV infection were recruited and studied at baseline and at 48 and 144 weeks. Patients were distributed into two groups according to baseline CD4(+) T-cell number below or above 200 cells/µL. Patients with fewer than 200 cells/µL were distributed into the responders and nonresponders according to antiretroviral therapy (ART) response at 144 weeks. Glycoprotein concentrations were determined by (1)H-NMR arising from the protein bond N-acetylglucosamine and N-acetylgalactosamine signals (GlycA); and N-acetylneuraminic acid signal (GlycB) associated with the sugar–protein bond concentration and aggregation state (shapes (height/width)). Basal glycoprotein concentrations were higher in patients with < 200 CD4(+) T-cell/μL (Glyc A: 1040(917.9–1199.1) vs. 950.4(845.5–1050.9), p < 0.001, and Glyc B: 521(440.3–610.3) vs. 468.6(417.9–507.0) μ mol/L, p < 0.001) being reduced by ART. The height/width (H/W) ratio was the parameter showing a better association with this clinical status. Baseline glycoproteins predict the condition of responder/nonresponder. In this study, (1)H-NMR glycoproteins provide novel insights to assess inflammation status and have prognostic value in PLWH. |
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