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Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer
Background: Recent studies showed a relevant role of hematogenous spread in ovarian cancer and the interest of circulating tumor cells (CTCs) monitoring as a prognosis marker. The aim of the present study was the characterization of CTCs from ovarian cancer patients, paying special attention to cell...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291036/ https://www.ncbi.nlm.nih.gov/pubmed/32423054 http://dx.doi.org/10.3390/cells9051218 |
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author | Abreu, Manuel Cabezas-Sainz, Pablo Alonso-Alconada, Lorena Ferreirós, Alba Mondelo-Macía, Patricia Lago-Lestón, Ramón Manuel Abalo, Alicia Díaz, Eva Palacios-Zambrano, Sara Rojo-Sebastian, Alejandro López-López, Rafael Sánchez, Laura Moreno-Bueno, Gema Muinelo-Romay, Laura |
author_facet | Abreu, Manuel Cabezas-Sainz, Pablo Alonso-Alconada, Lorena Ferreirós, Alba Mondelo-Macía, Patricia Lago-Lestón, Ramón Manuel Abalo, Alicia Díaz, Eva Palacios-Zambrano, Sara Rojo-Sebastian, Alejandro López-López, Rafael Sánchez, Laura Moreno-Bueno, Gema Muinelo-Romay, Laura |
author_sort | Abreu, Manuel |
collection | PubMed |
description | Background: Recent studies showed a relevant role of hematogenous spread in ovarian cancer and the interest of circulating tumor cells (CTCs) monitoring as a prognosis marker. The aim of the present study was the characterization of CTCs from ovarian cancer patients, paying special attention to cell plasticity characteristics to better understand the biology of these cells. Methods: CTCs isolation was carried out in 38 patients with advanced high-grade serous ovarian cancer using in parallel CellSearch and an alternative EpCAM-based immunoisolation followed by RT-qPCR analysis to characterize these cells. Results: Epithelial CTCs were found in 21% of patients, being their presence higher in patients with extraperitoneal metastasis. Importantly, this population was characterized by the expression of epithelial markers as MUC1 and CK19, but also by genes associated with mesenchymal and more malignant features as TIMP1, CXCR4 and the stem markers CD24 and CD44. In addition, we evidenced the relevance of TIMP1 expression to promote tumor proliferation, suggesting its interest as a therapeutic target. Conclusions: Overall, we evidenced the utility of the molecular characterization of EpCAM(+) CTCs from advanced ovarian cancer patients to identify biomarkers with potential applicability for disseminated disease detection and as therapeutic targets such as TIMP1. |
format | Online Article Text |
id | pubmed-7291036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72910362020-06-17 Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer Abreu, Manuel Cabezas-Sainz, Pablo Alonso-Alconada, Lorena Ferreirós, Alba Mondelo-Macía, Patricia Lago-Lestón, Ramón Manuel Abalo, Alicia Díaz, Eva Palacios-Zambrano, Sara Rojo-Sebastian, Alejandro López-López, Rafael Sánchez, Laura Moreno-Bueno, Gema Muinelo-Romay, Laura Cells Article Background: Recent studies showed a relevant role of hematogenous spread in ovarian cancer and the interest of circulating tumor cells (CTCs) monitoring as a prognosis marker. The aim of the present study was the characterization of CTCs from ovarian cancer patients, paying special attention to cell plasticity characteristics to better understand the biology of these cells. Methods: CTCs isolation was carried out in 38 patients with advanced high-grade serous ovarian cancer using in parallel CellSearch and an alternative EpCAM-based immunoisolation followed by RT-qPCR analysis to characterize these cells. Results: Epithelial CTCs were found in 21% of patients, being their presence higher in patients with extraperitoneal metastasis. Importantly, this population was characterized by the expression of epithelial markers as MUC1 and CK19, but also by genes associated with mesenchymal and more malignant features as TIMP1, CXCR4 and the stem markers CD24 and CD44. In addition, we evidenced the relevance of TIMP1 expression to promote tumor proliferation, suggesting its interest as a therapeutic target. Conclusions: Overall, we evidenced the utility of the molecular characterization of EpCAM(+) CTCs from advanced ovarian cancer patients to identify biomarkers with potential applicability for disseminated disease detection and as therapeutic targets such as TIMP1. MDPI 2020-05-14 /pmc/articles/PMC7291036/ /pubmed/32423054 http://dx.doi.org/10.3390/cells9051218 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Abreu, Manuel Cabezas-Sainz, Pablo Alonso-Alconada, Lorena Ferreirós, Alba Mondelo-Macía, Patricia Lago-Lestón, Ramón Manuel Abalo, Alicia Díaz, Eva Palacios-Zambrano, Sara Rojo-Sebastian, Alejandro López-López, Rafael Sánchez, Laura Moreno-Bueno, Gema Muinelo-Romay, Laura Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer |
title | Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer |
title_full | Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer |
title_fullStr | Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer |
title_full_unstemmed | Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer |
title_short | Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer |
title_sort | circulating tumor cells characterization revealed timp1 as a potential therapeutic target in ovarian cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291036/ https://www.ncbi.nlm.nih.gov/pubmed/32423054 http://dx.doi.org/10.3390/cells9051218 |
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