Cargando…
Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers
In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and deve...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291080/ https://www.ncbi.nlm.nih.gov/pubmed/32455542 http://dx.doi.org/10.3390/cells9051265 |
_version_ | 1783545826411282432 |
---|---|
author | Chermuła, Błażej Kranc, Wiesława Jopek, Karol Budna-Tukan, Joanna Hutchings, Greg Dompe, Claudia Moncrieff, Lisa Janowicz, Krzysztof Józkowiak, Małgorzata Jeseta, Michal Petitte, Jim Mozdziak, Paul Pawelczyk, Leszek Spaczyński, Robert Z. Kempisty, Bartosz |
author_facet | Chermuła, Błażej Kranc, Wiesława Jopek, Karol Budna-Tukan, Joanna Hutchings, Greg Dompe, Claudia Moncrieff, Lisa Janowicz, Krzysztof Józkowiak, Małgorzata Jeseta, Michal Petitte, Jim Mozdziak, Paul Pawelczyk, Leszek Spaczyński, Robert Z. Kempisty, Bartosz |
author_sort | Chermuła, Błażej |
collection | PubMed |
description | In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. Long-term in vitro culture of CCs collected from patients undergoing controlled ovarian stimulation during in vitro fertilization procedure was conducted. Using microarray expression analysis, transcript levels were assessed on day 1, 7, 15, and 30 of culture. Apoptosis and aging of CCs strictly influence oocyte quality and subsequently the outcome of assisted reproductive technologies (ART). Thus, particular attention was paid to the analysis of genes involved in programmed cell death, aging, and apoptosis. Due to the detailed level of expression analysis of each of the 133 analyzed genes, three groups were selected: first with significantly decreased expression during the culture; second with the statistically lowest increase in expression; and third with the highest significant increase in expression. COL3A1, SFRP4, CTGF, HTR2B, VCAM1, TNFRSF11B genes, belonging to the third group, were identified as potential carriers of information on oocyte quality. |
format | Online Article Text |
id | pubmed-7291080 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72910802020-06-17 Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers Chermuła, Błażej Kranc, Wiesława Jopek, Karol Budna-Tukan, Joanna Hutchings, Greg Dompe, Claudia Moncrieff, Lisa Janowicz, Krzysztof Józkowiak, Małgorzata Jeseta, Michal Petitte, Jim Mozdziak, Paul Pawelczyk, Leszek Spaczyński, Robert Z. Kempisty, Bartosz Cells Article In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. Long-term in vitro culture of CCs collected from patients undergoing controlled ovarian stimulation during in vitro fertilization procedure was conducted. Using microarray expression analysis, transcript levels were assessed on day 1, 7, 15, and 30 of culture. Apoptosis and aging of CCs strictly influence oocyte quality and subsequently the outcome of assisted reproductive technologies (ART). Thus, particular attention was paid to the analysis of genes involved in programmed cell death, aging, and apoptosis. Due to the detailed level of expression analysis of each of the 133 analyzed genes, three groups were selected: first with significantly decreased expression during the culture; second with the statistically lowest increase in expression; and third with the highest significant increase in expression. COL3A1, SFRP4, CTGF, HTR2B, VCAM1, TNFRSF11B genes, belonging to the third group, were identified as potential carriers of information on oocyte quality. MDPI 2020-05-21 /pmc/articles/PMC7291080/ /pubmed/32455542 http://dx.doi.org/10.3390/cells9051265 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chermuła, Błażej Kranc, Wiesława Jopek, Karol Budna-Tukan, Joanna Hutchings, Greg Dompe, Claudia Moncrieff, Lisa Janowicz, Krzysztof Józkowiak, Małgorzata Jeseta, Michal Petitte, Jim Mozdziak, Paul Pawelczyk, Leszek Spaczyński, Robert Z. Kempisty, Bartosz Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers |
title | Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers |
title_full | Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers |
title_fullStr | Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers |
title_full_unstemmed | Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers |
title_short | Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers |
title_sort | human cumulus cells in long-term in vitro culture reflect differential expression profile of genes responsible for planned cell death and aging—a study of new molecular markers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291080/ https://www.ncbi.nlm.nih.gov/pubmed/32455542 http://dx.doi.org/10.3390/cells9051265 |
work_keys_str_mv | AT chermułabłazej humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT krancwiesława humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT jopekkarol humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT budnatukanjoanna humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT hutchingsgreg humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT dompeclaudia humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT moncriefflisa humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT janowiczkrzysztof humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT jozkowiakmałgorzata humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT jesetamichal humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT petittejim humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT mozdziakpaul humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT pawelczykleszek humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT spaczynskirobertz humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers AT kempistybartosz humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers |