Cargando…

Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers

In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and deve...

Descripción completa

Detalles Bibliográficos
Autores principales: Chermuła, Błażej, Kranc, Wiesława, Jopek, Karol, Budna-Tukan, Joanna, Hutchings, Greg, Dompe, Claudia, Moncrieff, Lisa, Janowicz, Krzysztof, Józkowiak, Małgorzata, Jeseta, Michal, Petitte, Jim, Mozdziak, Paul, Pawelczyk, Leszek, Spaczyński, Robert Z., Kempisty, Bartosz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291080/
https://www.ncbi.nlm.nih.gov/pubmed/32455542
http://dx.doi.org/10.3390/cells9051265
_version_ 1783545826411282432
author Chermuła, Błażej
Kranc, Wiesława
Jopek, Karol
Budna-Tukan, Joanna
Hutchings, Greg
Dompe, Claudia
Moncrieff, Lisa
Janowicz, Krzysztof
Józkowiak, Małgorzata
Jeseta, Michal
Petitte, Jim
Mozdziak, Paul
Pawelczyk, Leszek
Spaczyński, Robert Z.
Kempisty, Bartosz
author_facet Chermuła, Błażej
Kranc, Wiesława
Jopek, Karol
Budna-Tukan, Joanna
Hutchings, Greg
Dompe, Claudia
Moncrieff, Lisa
Janowicz, Krzysztof
Józkowiak, Małgorzata
Jeseta, Michal
Petitte, Jim
Mozdziak, Paul
Pawelczyk, Leszek
Spaczyński, Robert Z.
Kempisty, Bartosz
author_sort Chermuła, Błażej
collection PubMed
description In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. Long-term in vitro culture of CCs collected from patients undergoing controlled ovarian stimulation during in vitro fertilization procedure was conducted. Using microarray expression analysis, transcript levels were assessed on day 1, 7, 15, and 30 of culture. Apoptosis and aging of CCs strictly influence oocyte quality and subsequently the outcome of assisted reproductive technologies (ART). Thus, particular attention was paid to the analysis of genes involved in programmed cell death, aging, and apoptosis. Due to the detailed level of expression analysis of each of the 133 analyzed genes, three groups were selected: first with significantly decreased expression during the culture; second with the statistically lowest increase in expression; and third with the highest significant increase in expression. COL3A1, SFRP4, CTGF, HTR2B, VCAM1, TNFRSF11B genes, belonging to the third group, were identified as potential carriers of information on oocyte quality.
format Online
Article
Text
id pubmed-7291080
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72910802020-06-17 Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers Chermuła, Błażej Kranc, Wiesława Jopek, Karol Budna-Tukan, Joanna Hutchings, Greg Dompe, Claudia Moncrieff, Lisa Janowicz, Krzysztof Józkowiak, Małgorzata Jeseta, Michal Petitte, Jim Mozdziak, Paul Pawelczyk, Leszek Spaczyński, Robert Z. Kempisty, Bartosz Cells Article In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. Long-term in vitro culture of CCs collected from patients undergoing controlled ovarian stimulation during in vitro fertilization procedure was conducted. Using microarray expression analysis, transcript levels were assessed on day 1, 7, 15, and 30 of culture. Apoptosis and aging of CCs strictly influence oocyte quality and subsequently the outcome of assisted reproductive technologies (ART). Thus, particular attention was paid to the analysis of genes involved in programmed cell death, aging, and apoptosis. Due to the detailed level of expression analysis of each of the 133 analyzed genes, three groups were selected: first with significantly decreased expression during the culture; second with the statistically lowest increase in expression; and third with the highest significant increase in expression. COL3A1, SFRP4, CTGF, HTR2B, VCAM1, TNFRSF11B genes, belonging to the third group, were identified as potential carriers of information on oocyte quality. MDPI 2020-05-21 /pmc/articles/PMC7291080/ /pubmed/32455542 http://dx.doi.org/10.3390/cells9051265 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chermuła, Błażej
Kranc, Wiesława
Jopek, Karol
Budna-Tukan, Joanna
Hutchings, Greg
Dompe, Claudia
Moncrieff, Lisa
Janowicz, Krzysztof
Józkowiak, Małgorzata
Jeseta, Michal
Petitte, Jim
Mozdziak, Paul
Pawelczyk, Leszek
Spaczyński, Robert Z.
Kempisty, Bartosz
Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers
title Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers
title_full Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers
title_fullStr Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers
title_full_unstemmed Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers
title_short Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers
title_sort human cumulus cells in long-term in vitro culture reflect differential expression profile of genes responsible for planned cell death and aging—a study of new molecular markers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291080/
https://www.ncbi.nlm.nih.gov/pubmed/32455542
http://dx.doi.org/10.3390/cells9051265
work_keys_str_mv AT chermułabłazej humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT krancwiesława humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT jopekkarol humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT budnatukanjoanna humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT hutchingsgreg humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT dompeclaudia humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT moncriefflisa humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT janowiczkrzysztof humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT jozkowiakmałgorzata humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT jesetamichal humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT petittejim humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT mozdziakpaul humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT pawelczykleszek humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT spaczynskirobertz humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers
AT kempistybartosz humancumuluscellsinlongterminvitroculturereflectdifferentialexpressionprofileofgenesresponsibleforplannedcelldeathandagingastudyofnewmolecularmarkers