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Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis

To determine if a modification of the cytokine profile occurs in the follicular fluid (FF) of women with endometriosis undergoing in vitro fertilization (IVF), we performed a prospective observational study from January 2018 to February 2019. In total, 87 women undergoing IVF were included: 43 for s...

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Autores principales: Bouet, Pierre-Emmanuel, Chao de la Barca, Juan-Manuel, Boucret, Lisa, Descamps, Philippe, Legendre, Guillaume, Hachem, Hady El, Blanchard, Simon, Jeannin, Pascale, Reynier, Pascal, May-Panloup, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291127/
https://www.ncbi.nlm.nih.gov/pubmed/32370059
http://dx.doi.org/10.3390/jcm9051306
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author Bouet, Pierre-Emmanuel
Chao de la Barca, Juan-Manuel
Boucret, Lisa
Descamps, Philippe
Legendre, Guillaume
Hachem, Hady El
Blanchard, Simon
Jeannin, Pascale
Reynier, Pascal
May-Panloup, Pascale
author_facet Bouet, Pierre-Emmanuel
Chao de la Barca, Juan-Manuel
Boucret, Lisa
Descamps, Philippe
Legendre, Guillaume
Hachem, Hady El
Blanchard, Simon
Jeannin, Pascale
Reynier, Pascal
May-Panloup, Pascale
author_sort Bouet, Pierre-Emmanuel
collection PubMed
description To determine if a modification of the cytokine profile occurs in the follicular fluid (FF) of women with endometriosis undergoing in vitro fertilization (IVF), we performed a prospective observational study from January 2018 to February 2019. In total, 87 women undergoing IVF were included: 43 for severe endometriosis-related infertility and 40 controls with other causes of infertility. The cytokine profile of the FF was determined by multiplex fluorescent-bead-based technology allowing the measurement of 59 cytokines. Monocyte Chemoattractant Protein 1 (MCP-1) was the only variable retained in the multivariate analysis. We identified two subgroups of patients in the endometriosis group: MCP-1-low group (n = 23), which had FF MCP-1 levels comparable to the control group, and MCP-1-high (n = 20), which had significantly higher FF levels. Only patients in the MCP-1-high group had a significantly altered cytokine profile in the FF, and had a significantly higher serum estradiol level (p = 0.002) and a significantly lower number of oocytes recovered (p = 0.01) compared to the MCP-1-low and the control group. Our study has shown an alteration of the oocyte microenvironment in women with endometriosis associated with high follicular fluid levels of MCP-1, allowing the identification of a subgroup of endometriosis patients with a potentially worse prognosis.
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spelling pubmed-72911272020-06-17 Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis Bouet, Pierre-Emmanuel Chao de la Barca, Juan-Manuel Boucret, Lisa Descamps, Philippe Legendre, Guillaume Hachem, Hady El Blanchard, Simon Jeannin, Pascale Reynier, Pascal May-Panloup, Pascale J Clin Med Article To determine if a modification of the cytokine profile occurs in the follicular fluid (FF) of women with endometriosis undergoing in vitro fertilization (IVF), we performed a prospective observational study from January 2018 to February 2019. In total, 87 women undergoing IVF were included: 43 for severe endometriosis-related infertility and 40 controls with other causes of infertility. The cytokine profile of the FF was determined by multiplex fluorescent-bead-based technology allowing the measurement of 59 cytokines. Monocyte Chemoattractant Protein 1 (MCP-1) was the only variable retained in the multivariate analysis. We identified two subgroups of patients in the endometriosis group: MCP-1-low group (n = 23), which had FF MCP-1 levels comparable to the control group, and MCP-1-high (n = 20), which had significantly higher FF levels. Only patients in the MCP-1-high group had a significantly altered cytokine profile in the FF, and had a significantly higher serum estradiol level (p = 0.002) and a significantly lower number of oocytes recovered (p = 0.01) compared to the MCP-1-low and the control group. Our study has shown an alteration of the oocyte microenvironment in women with endometriosis associated with high follicular fluid levels of MCP-1, allowing the identification of a subgroup of endometriosis patients with a potentially worse prognosis. MDPI 2020-05-01 /pmc/articles/PMC7291127/ /pubmed/32370059 http://dx.doi.org/10.3390/jcm9051306 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bouet, Pierre-Emmanuel
Chao de la Barca, Juan-Manuel
Boucret, Lisa
Descamps, Philippe
Legendre, Guillaume
Hachem, Hady El
Blanchard, Simon
Jeannin, Pascale
Reynier, Pascal
May-Panloup, Pascale
Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis
title Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis
title_full Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis
title_fullStr Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis
title_full_unstemmed Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis
title_short Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis
title_sort elevated levels of monocyte chemotactic protein-1 in the follicular fluid reveals different populations among women with severe endometriosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291127/
https://www.ncbi.nlm.nih.gov/pubmed/32370059
http://dx.doi.org/10.3390/jcm9051306
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