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Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization

The presence of circulating tumor cells (CTCs) in patients with solid tumors is associated with poor prognosis. However, there are limited data concerning the detection of CTCs in renal cell cancer (RCC). The aim of this study is to evaluate the presence of CTCs in peripheral blood of patients with...

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Autores principales: Klezl, Petr, Pospisilova, Eliska, Kolostova, Katarina, Sonsky, Jindrich, Maly, Ondrej, Grill, Robert, Pawlak, Ireneusz, Bobek, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291128/
https://www.ncbi.nlm.nih.gov/pubmed/32392757
http://dx.doi.org/10.3390/jcm9051372
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author Klezl, Petr
Pospisilova, Eliska
Kolostova, Katarina
Sonsky, Jindrich
Maly, Ondrej
Grill, Robert
Pawlak, Ireneusz
Bobek, Vladimir
author_facet Klezl, Petr
Pospisilova, Eliska
Kolostova, Katarina
Sonsky, Jindrich
Maly, Ondrej
Grill, Robert
Pawlak, Ireneusz
Bobek, Vladimir
author_sort Klezl, Petr
collection PubMed
description The presence of circulating tumor cells (CTCs) in patients with solid tumors is associated with poor prognosis. However, there are limited data concerning the detection of CTCs in renal cell cancer (RCC). The aim of this study is to evaluate the presence of CTCs in peripheral blood of patients with RCC undergoing surgery (n = 186). CTCs were tested before and after surgery as well as during the follow-up period afterwards. In total 495 CTC testing in duplicates were provided. To enrich CTCs, a size-based separation protocol and tube MetaCell® was used. CTCs presence was evaluated by single cell cytomorphology based on vital fluorescence microscopy. Additionally, to standardly applied fluorescence stains, CTCs viability was controlled by mitochondrial activity. CTCs were detected independently on the sampling order in up to 86.7% of the tested blood samples in patients undergoing RCC surgery. There is higher probability of CTC detection with growing tumor size, especially in clear cell renal cell cancer (ccRCC) cases. Similarly, the tumor size corresponds with metastasis presence and lymph node positivity and CTC detection. This paper describes for the first-time successful analysis of viable CTCs and their mitochondria as a part of the functional characterization of CTCs in RCC.
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spelling pubmed-72911282020-06-17 Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization Klezl, Petr Pospisilova, Eliska Kolostova, Katarina Sonsky, Jindrich Maly, Ondrej Grill, Robert Pawlak, Ireneusz Bobek, Vladimir J Clin Med Article The presence of circulating tumor cells (CTCs) in patients with solid tumors is associated with poor prognosis. However, there are limited data concerning the detection of CTCs in renal cell cancer (RCC). The aim of this study is to evaluate the presence of CTCs in peripheral blood of patients with RCC undergoing surgery (n = 186). CTCs were tested before and after surgery as well as during the follow-up period afterwards. In total 495 CTC testing in duplicates were provided. To enrich CTCs, a size-based separation protocol and tube MetaCell® was used. CTCs presence was evaluated by single cell cytomorphology based on vital fluorescence microscopy. Additionally, to standardly applied fluorescence stains, CTCs viability was controlled by mitochondrial activity. CTCs were detected independently on the sampling order in up to 86.7% of the tested blood samples in patients undergoing RCC surgery. There is higher probability of CTC detection with growing tumor size, especially in clear cell renal cell cancer (ccRCC) cases. Similarly, the tumor size corresponds with metastasis presence and lymph node positivity and CTC detection. This paper describes for the first-time successful analysis of viable CTCs and their mitochondria as a part of the functional characterization of CTCs in RCC. MDPI 2020-05-07 /pmc/articles/PMC7291128/ /pubmed/32392757 http://dx.doi.org/10.3390/jcm9051372 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Klezl, Petr
Pospisilova, Eliska
Kolostova, Katarina
Sonsky, Jindrich
Maly, Ondrej
Grill, Robert
Pawlak, Ireneusz
Bobek, Vladimir
Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization
title Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization
title_full Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization
title_fullStr Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization
title_full_unstemmed Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization
title_short Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization
title_sort detection of circulating tumor cells in renal cell carcinoma: disease stage correlation and molecular characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291128/
https://www.ncbi.nlm.nih.gov/pubmed/32392757
http://dx.doi.org/10.3390/jcm9051372
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