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Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood

Dalbergia odorifera, a critically endangered tree species, produces heartwood containing a vast variety of flavonoids. This heartwood, also known as Chinese rosewood, has high economic and medicinal value, but its formation takes several decades. In this study, we showed that discolored wood induced...

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Autores principales: Sun, Ying, Gao, Mei, Kang, Seogchan, Yang, Chengmin, Meng, Hui, Yang, Yun, Zhao, Xiangsheng, Gao, Zhihui, Xu, Yanhong, Jin, Yue, Zhao, Xiaohong, Zhang, Zheng, Han, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291145/
https://www.ncbi.nlm.nih.gov/pubmed/32353985
http://dx.doi.org/10.3390/genes11050478
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author Sun, Ying
Gao, Mei
Kang, Seogchan
Yang, Chengmin
Meng, Hui
Yang, Yun
Zhao, Xiangsheng
Gao, Zhihui
Xu, Yanhong
Jin, Yue
Zhao, Xiaohong
Zhang, Zheng
Han, Jianping
author_facet Sun, Ying
Gao, Mei
Kang, Seogchan
Yang, Chengmin
Meng, Hui
Yang, Yun
Zhao, Xiangsheng
Gao, Zhihui
Xu, Yanhong
Jin, Yue
Zhao, Xiaohong
Zhang, Zheng
Han, Jianping
author_sort Sun, Ying
collection PubMed
description Dalbergia odorifera, a critically endangered tree species, produces heartwood containing a vast variety of flavonoids. This heartwood, also known as Chinese rosewood, has high economic and medicinal value, but its formation takes several decades. In this study, we showed that discolored wood induced by pruning displays similar color, structure, and flavonoids content to those of natural heartwood, suggesting that wounding is an efficient method for inducing flavonoid production in D. odorifera. Transcriptome analysis was performed to investigate the mechanism underlying wounding-induced flavonoids production in D. odorifera heartwood. Wounding upregulated the expression of 90 unigenes, which covered 19 gene families of the phenylpropanoid and flavonoid pathways, including PAL, C4H, 4CL, CHS, CHI, 6DCS, F3’5’H, F3H, FMO, GT, PMAT, CHOMT, IFS, HI4’OMT, HID, IOMT, I2’H, IFR, and I3’H. Furthermore, 47 upregulated unigenes were mapped to the biosynthesis pathways for five signal molecules (ET, JA, ABA, ROS, and SA). Exogenous application of these signal molecules resulted in the accumulation of flavonoids in cell suspensions of D. odorifera, supporting their role in wounding-induced flavonoid production. Insights from this study will help develop new methods for rapidly inducing the formation of heartwood with enhanced medicinal value.
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spelling pubmed-72911452020-06-17 Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood Sun, Ying Gao, Mei Kang, Seogchan Yang, Chengmin Meng, Hui Yang, Yun Zhao, Xiangsheng Gao, Zhihui Xu, Yanhong Jin, Yue Zhao, Xiaohong Zhang, Zheng Han, Jianping Genes (Basel) Article Dalbergia odorifera, a critically endangered tree species, produces heartwood containing a vast variety of flavonoids. This heartwood, also known as Chinese rosewood, has high economic and medicinal value, but its formation takes several decades. In this study, we showed that discolored wood induced by pruning displays similar color, structure, and flavonoids content to those of natural heartwood, suggesting that wounding is an efficient method for inducing flavonoid production in D. odorifera. Transcriptome analysis was performed to investigate the mechanism underlying wounding-induced flavonoids production in D. odorifera heartwood. Wounding upregulated the expression of 90 unigenes, which covered 19 gene families of the phenylpropanoid and flavonoid pathways, including PAL, C4H, 4CL, CHS, CHI, 6DCS, F3’5’H, F3H, FMO, GT, PMAT, CHOMT, IFS, HI4’OMT, HID, IOMT, I2’H, IFR, and I3’H. Furthermore, 47 upregulated unigenes were mapped to the biosynthesis pathways for five signal molecules (ET, JA, ABA, ROS, and SA). Exogenous application of these signal molecules resulted in the accumulation of flavonoids in cell suspensions of D. odorifera, supporting their role in wounding-induced flavonoid production. Insights from this study will help develop new methods for rapidly inducing the formation of heartwood with enhanced medicinal value. MDPI 2020-04-28 /pmc/articles/PMC7291145/ /pubmed/32353985 http://dx.doi.org/10.3390/genes11050478 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Ying
Gao, Mei
Kang, Seogchan
Yang, Chengmin
Meng, Hui
Yang, Yun
Zhao, Xiangsheng
Gao, Zhihui
Xu, Yanhong
Jin, Yue
Zhao, Xiaohong
Zhang, Zheng
Han, Jianping
Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood
title Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood
title_full Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood
title_fullStr Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood
title_full_unstemmed Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood
title_short Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood
title_sort molecular mechanism underlying mechanical wounding-induced flavonoid accumulation in dalbergia odorifera t. chen, an endangered tree that produces chinese rosewood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291145/
https://www.ncbi.nlm.nih.gov/pubmed/32353985
http://dx.doi.org/10.3390/genes11050478
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