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Blood Eosinophils Subtypes and Their Survivability in Asthma Patients

Eosinophils subtypes as lung-resident (rEOS) and inflammatory (iEOS) eosinophils are different in surface protein expression, functions, response to IL-5 and localization in lungs. rEOS- and iEOS-like eosinophils are found in blood; thus, we aimed to investigate their quantity and survivability in a...

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Autores principales: Januskevicius, Andrius, Jurkeviciute, Egle, Janulaityte, Ieva, Kalinauskaite-Zukauske, Virginija, Miliauskas, Skaidrius, Malakauskas, Kestutis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291159/
https://www.ncbi.nlm.nih.gov/pubmed/32443594
http://dx.doi.org/10.3390/cells9051248
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author Januskevicius, Andrius
Jurkeviciute, Egle
Janulaityte, Ieva
Kalinauskaite-Zukauske, Virginija
Miliauskas, Skaidrius
Malakauskas, Kestutis
author_facet Januskevicius, Andrius
Jurkeviciute, Egle
Janulaityte, Ieva
Kalinauskaite-Zukauske, Virginija
Miliauskas, Skaidrius
Malakauskas, Kestutis
author_sort Januskevicius, Andrius
collection PubMed
description Eosinophils subtypes as lung-resident (rEOS) and inflammatory (iEOS) eosinophils are different in surface protein expression, functions, response to IL-5 and localization in lungs. rEOS- and iEOS-like eosinophils are found in blood; thus, we aimed to investigate their quantity and survivability in asthma patients. A total of 40 individuals were included: 10 steroid-free non-severe allergic asthma (AA), and 18 severe non-allergic eosinophilic asthma (SNEA) patients, the control group consisted of 12 healthy non-smoking subjects (HS). A bronchial challenge with Dermatophagoides pteronysinnus allergen was performed for AA patients and HS. Blood eosinophils subtyping was completed with magnetic beads’ conjugated antibodies against surface CD62L. Eosinophils adhesion to hTERT airway smooth muscle (ASM) cells was measured by evaluating their peroxidase activity and viability by annexin V and propidium iodide staining. We found that the predominant blood eosinophil subtype in AA patients was iEOS, while rEOS prevailed in SNEA patients (p < 0.05). Moreover, rEOS demonstrated higher adhesion intensity compared with iEOS in all investigated groups. Both eosinophils subtypes of SNEA patients had higher survivability over the AA group. However, iEOS survivability from AA and SNEA groups was higher compared with rEOS under standard conditions, when rEOS survivability increased after their incubation with ASM cells. Bronchial allergen challenge abolished the dominance of blood iEOS in AA patients and prolonged only iEOS survivability. Though the challenge did not affect the adhesion of any eosinophils subtypes, the direct dependence of rEOS and iEOS survivability on their interaction with ASM cells was revealed (p < 0.05). These findings provide the premise for eosinophils subtype-oriented asthma treatment.
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spelling pubmed-72911592020-06-17 Blood Eosinophils Subtypes and Their Survivability in Asthma Patients Januskevicius, Andrius Jurkeviciute, Egle Janulaityte, Ieva Kalinauskaite-Zukauske, Virginija Miliauskas, Skaidrius Malakauskas, Kestutis Cells Article Eosinophils subtypes as lung-resident (rEOS) and inflammatory (iEOS) eosinophils are different in surface protein expression, functions, response to IL-5 and localization in lungs. rEOS- and iEOS-like eosinophils are found in blood; thus, we aimed to investigate their quantity and survivability in asthma patients. A total of 40 individuals were included: 10 steroid-free non-severe allergic asthma (AA), and 18 severe non-allergic eosinophilic asthma (SNEA) patients, the control group consisted of 12 healthy non-smoking subjects (HS). A bronchial challenge with Dermatophagoides pteronysinnus allergen was performed for AA patients and HS. Blood eosinophils subtyping was completed with magnetic beads’ conjugated antibodies against surface CD62L. Eosinophils adhesion to hTERT airway smooth muscle (ASM) cells was measured by evaluating their peroxidase activity and viability by annexin V and propidium iodide staining. We found that the predominant blood eosinophil subtype in AA patients was iEOS, while rEOS prevailed in SNEA patients (p < 0.05). Moreover, rEOS demonstrated higher adhesion intensity compared with iEOS in all investigated groups. Both eosinophils subtypes of SNEA patients had higher survivability over the AA group. However, iEOS survivability from AA and SNEA groups was higher compared with rEOS under standard conditions, when rEOS survivability increased after their incubation with ASM cells. Bronchial allergen challenge abolished the dominance of blood iEOS in AA patients and prolonged only iEOS survivability. Though the challenge did not affect the adhesion of any eosinophils subtypes, the direct dependence of rEOS and iEOS survivability on their interaction with ASM cells was revealed (p < 0.05). These findings provide the premise for eosinophils subtype-oriented asthma treatment. MDPI 2020-05-18 /pmc/articles/PMC7291159/ /pubmed/32443594 http://dx.doi.org/10.3390/cells9051248 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Januskevicius, Andrius
Jurkeviciute, Egle
Janulaityte, Ieva
Kalinauskaite-Zukauske, Virginija
Miliauskas, Skaidrius
Malakauskas, Kestutis
Blood Eosinophils Subtypes and Their Survivability in Asthma Patients
title Blood Eosinophils Subtypes and Their Survivability in Asthma Patients
title_full Blood Eosinophils Subtypes and Their Survivability in Asthma Patients
title_fullStr Blood Eosinophils Subtypes and Their Survivability in Asthma Patients
title_full_unstemmed Blood Eosinophils Subtypes and Their Survivability in Asthma Patients
title_short Blood Eosinophils Subtypes and Their Survivability in Asthma Patients
title_sort blood eosinophils subtypes and their survivability in asthma patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291159/
https://www.ncbi.nlm.nih.gov/pubmed/32443594
http://dx.doi.org/10.3390/cells9051248
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