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Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study

Surfaces of implanted medical devices are highly susceptible to biofilm formation. Bacteria in biofilms are embedded in a self-produced extracellular matrix that inhibits the penetration of antibiotics and significantly contributes to the mechanical stability of the colonizing community which leads...

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Autores principales: Fursov, Mikhail V., Abdrakhmanova, Radmila O., Antonova, Nataliia P., Vasina, Daria V., Kolchanova, Anastasia D., Bashkina, Olga A., Rubalsky, Oleg V., Samotrueva, Marina A., Potapov, Vasiliy D., Makarov, Valentine V., Yudin, Sergey M., Gintsburg, Alexander L., Tkachuk, Artem P., Gushchin, Vladimir A., Rubalskii, Evgenii O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291189/
https://www.ncbi.nlm.nih.gov/pubmed/32429199
http://dx.doi.org/10.3390/v12050545
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author Fursov, Mikhail V.
Abdrakhmanova, Radmila O.
Antonova, Nataliia P.
Vasina, Daria V.
Kolchanova, Anastasia D.
Bashkina, Olga A.
Rubalsky, Oleg V.
Samotrueva, Marina A.
Potapov, Vasiliy D.
Makarov, Valentine V.
Yudin, Sergey M.
Gintsburg, Alexander L.
Tkachuk, Artem P.
Gushchin, Vladimir A.
Rubalskii, Evgenii O.
author_facet Fursov, Mikhail V.
Abdrakhmanova, Radmila O.
Antonova, Nataliia P.
Vasina, Daria V.
Kolchanova, Anastasia D.
Bashkina, Olga A.
Rubalsky, Oleg V.
Samotrueva, Marina A.
Potapov, Vasiliy D.
Makarov, Valentine V.
Yudin, Sergey M.
Gintsburg, Alexander L.
Tkachuk, Artem P.
Gushchin, Vladimir A.
Rubalskii, Evgenii O.
author_sort Fursov, Mikhail V.
collection PubMed
description Surfaces of implanted medical devices are highly susceptible to biofilm formation. Bacteria in biofilms are embedded in a self-produced extracellular matrix that inhibits the penetration of antibiotics and significantly contributes to the mechanical stability of the colonizing community which leads to an increase in morbidity and mortality rate in clinical settings. Therefore, new antibiofilm approaches and substances are urgently needed. In this paper, we test the efficacy of a broad-range recombinant endolysin of the coliphage LysECD7 against forming and mature biofilms. We used a strong biofilm producer—Klebsiella pneumoniae Ts 141-14 clinical isolate. In vitro investigation of the antibacterial activity was performed using the standard biofilm assay in microtiter plates. We optimized the implantable diffusion chamber approach in order to reach strong biofilm formation in vivo avoiding severe consequences of the pathogen for the animals and to obtain a well-reproducible model of implant-associated infection. Endolysin LysECD7 significantly reduced the biofilm formation and was capable of degrading the preformed biofilm in vitro. The animal trials on the preformed biofilms confirmed these results. Overall, our results show that LysECD7 is a promising substance against clinically relevant biofilms.
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spelling pubmed-72911892020-06-17 Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study Fursov, Mikhail V. Abdrakhmanova, Radmila O. Antonova, Nataliia P. Vasina, Daria V. Kolchanova, Anastasia D. Bashkina, Olga A. Rubalsky, Oleg V. Samotrueva, Marina A. Potapov, Vasiliy D. Makarov, Valentine V. Yudin, Sergey M. Gintsburg, Alexander L. Tkachuk, Artem P. Gushchin, Vladimir A. Rubalskii, Evgenii O. Viruses Article Surfaces of implanted medical devices are highly susceptible to biofilm formation. Bacteria in biofilms are embedded in a self-produced extracellular matrix that inhibits the penetration of antibiotics and significantly contributes to the mechanical stability of the colonizing community which leads to an increase in morbidity and mortality rate in clinical settings. Therefore, new antibiofilm approaches and substances are urgently needed. In this paper, we test the efficacy of a broad-range recombinant endolysin of the coliphage LysECD7 against forming and mature biofilms. We used a strong biofilm producer—Klebsiella pneumoniae Ts 141-14 clinical isolate. In vitro investigation of the antibacterial activity was performed using the standard biofilm assay in microtiter plates. We optimized the implantable diffusion chamber approach in order to reach strong biofilm formation in vivo avoiding severe consequences of the pathogen for the animals and to obtain a well-reproducible model of implant-associated infection. Endolysin LysECD7 significantly reduced the biofilm formation and was capable of degrading the preformed biofilm in vitro. The animal trials on the preformed biofilms confirmed these results. Overall, our results show that LysECD7 is a promising substance against clinically relevant biofilms. MDPI 2020-05-15 /pmc/articles/PMC7291189/ /pubmed/32429199 http://dx.doi.org/10.3390/v12050545 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fursov, Mikhail V.
Abdrakhmanova, Radmila O.
Antonova, Nataliia P.
Vasina, Daria V.
Kolchanova, Anastasia D.
Bashkina, Olga A.
Rubalsky, Oleg V.
Samotrueva, Marina A.
Potapov, Vasiliy D.
Makarov, Valentine V.
Yudin, Sergey M.
Gintsburg, Alexander L.
Tkachuk, Artem P.
Gushchin, Vladimir A.
Rubalskii, Evgenii O.
Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study
title Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study
title_full Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study
title_fullStr Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study
title_full_unstemmed Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study
title_short Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study
title_sort antibiofilm activity of a broad-range recombinant endolysin lysecd7: in vitro and in vivo study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291189/
https://www.ncbi.nlm.nih.gov/pubmed/32429199
http://dx.doi.org/10.3390/v12050545
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