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Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum

The cellular mechanisms that ensure the selectivity and fidelity of secretory cargo protein transport from the endoplasmic reticulum (ER) to the Golgi are still not well understood. The p24 protein complex acts as a specific cargo receptor for GPI-anchored proteins by facilitating their ER exit thro...

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Autores principales: Lopez, Sergio, Perez-Linero, Ana Maria, Manzano-Lopez, Javier, Sabido-Bozo, Susana, Cortes-Gomez, Alejandro, Rodriguez-Gallardo, Sofia, Aguilera-Romero, Auxiliadora, Goder, Veit, Muñiz, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291304/
https://www.ncbi.nlm.nih.gov/pubmed/32456004
http://dx.doi.org/10.3390/cells9051295
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author Lopez, Sergio
Perez-Linero, Ana Maria
Manzano-Lopez, Javier
Sabido-Bozo, Susana
Cortes-Gomez, Alejandro
Rodriguez-Gallardo, Sofia
Aguilera-Romero, Auxiliadora
Goder, Veit
Muñiz, Manuel
author_facet Lopez, Sergio
Perez-Linero, Ana Maria
Manzano-Lopez, Javier
Sabido-Bozo, Susana
Cortes-Gomez, Alejandro
Rodriguez-Gallardo, Sofia
Aguilera-Romero, Auxiliadora
Goder, Veit
Muñiz, Manuel
author_sort Lopez, Sergio
collection PubMed
description The cellular mechanisms that ensure the selectivity and fidelity of secretory cargo protein transport from the endoplasmic reticulum (ER) to the Golgi are still not well understood. The p24 protein complex acts as a specific cargo receptor for GPI-anchored proteins by facilitating their ER exit through a specialized export pathway in yeast. In parallel, the p24 complex can also exit the ER using the general pathway that exports the rest of secretory proteins with their respective cargo receptors. Here, we show biochemically that the p24 complex associates at the ER with other cargo receptors in a COPII-dependent manner, forming high-molecular weight multireceptor complexes. Furthermore, live cell imaging analysis reveals that the p24 complex is required to retain in the ER secretory cargos when their specific receptors are absent. This requirement does not involve neither the unfolded protein response nor the retrograde transport from the Golgi. Our results suggest that, in addition to its role as a cargo receptor in the specialized GPI-anchored protein pathway, the p24 complex also plays an independent role in secretory cargo selectivity during its exit through the general ER export pathway, preventing the non-selective bulk flow of native secretory cargos. This mechanism would ensure receptor-regulated cargo transport, providing an additional layer of regulation of secretory cargo selectivity during ER export.
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spelling pubmed-72913042020-06-17 Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum Lopez, Sergio Perez-Linero, Ana Maria Manzano-Lopez, Javier Sabido-Bozo, Susana Cortes-Gomez, Alejandro Rodriguez-Gallardo, Sofia Aguilera-Romero, Auxiliadora Goder, Veit Muñiz, Manuel Cells Article The cellular mechanisms that ensure the selectivity and fidelity of secretory cargo protein transport from the endoplasmic reticulum (ER) to the Golgi are still not well understood. The p24 protein complex acts as a specific cargo receptor for GPI-anchored proteins by facilitating their ER exit through a specialized export pathway in yeast. In parallel, the p24 complex can also exit the ER using the general pathway that exports the rest of secretory proteins with their respective cargo receptors. Here, we show biochemically that the p24 complex associates at the ER with other cargo receptors in a COPII-dependent manner, forming high-molecular weight multireceptor complexes. Furthermore, live cell imaging analysis reveals that the p24 complex is required to retain in the ER secretory cargos when their specific receptors are absent. This requirement does not involve neither the unfolded protein response nor the retrograde transport from the Golgi. Our results suggest that, in addition to its role as a cargo receptor in the specialized GPI-anchored protein pathway, the p24 complex also plays an independent role in secretory cargo selectivity during its exit through the general ER export pathway, preventing the non-selective bulk flow of native secretory cargos. This mechanism would ensure receptor-regulated cargo transport, providing an additional layer of regulation of secretory cargo selectivity during ER export. MDPI 2020-05-22 /pmc/articles/PMC7291304/ /pubmed/32456004 http://dx.doi.org/10.3390/cells9051295 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lopez, Sergio
Perez-Linero, Ana Maria
Manzano-Lopez, Javier
Sabido-Bozo, Susana
Cortes-Gomez, Alejandro
Rodriguez-Gallardo, Sofia
Aguilera-Romero, Auxiliadora
Goder, Veit
Muñiz, Manuel
Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_full Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_fullStr Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_full_unstemmed Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_short Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_sort dual independent roles of the p24 complex in selectivity of secretory cargo export from the endoplasmic reticulum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291304/
https://www.ncbi.nlm.nih.gov/pubmed/32456004
http://dx.doi.org/10.3390/cells9051295
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