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Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury

BACKGROUND: Peripheral nerve injury is a debilitating condition that may lead to partial or complete motor, sensory and autonomic function loss and lacks effective therapy until date. Therefore, it is quite imperative to explore impending remedies for rapid and accurate functional retrieval followin...

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Autores principales: Razzaq, Aroona, Hussain, Ghulam, Rasul, Azhar, Xu, Jiaqi, Zhang, Qiqi, Malik, Shoaib Ahmad, Anwar, Haseeb, Aziz, Nimra, Braidy, Nady, de Aguilar, Jose-Luis Gonzalez, Wei, Wei, Li, Jiang, Li, Xiaomeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291632/
https://www.ncbi.nlm.nih.gov/pubmed/32527244
http://dx.doi.org/10.1186/s12906-020-02950-3
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author Razzaq, Aroona
Hussain, Ghulam
Rasul, Azhar
Xu, Jiaqi
Zhang, Qiqi
Malik, Shoaib Ahmad
Anwar, Haseeb
Aziz, Nimra
Braidy, Nady
de Aguilar, Jose-Luis Gonzalez
Wei, Wei
Li, Jiang
Li, Xiaomeng
author_facet Razzaq, Aroona
Hussain, Ghulam
Rasul, Azhar
Xu, Jiaqi
Zhang, Qiqi
Malik, Shoaib Ahmad
Anwar, Haseeb
Aziz, Nimra
Braidy, Nady
de Aguilar, Jose-Luis Gonzalez
Wei, Wei
Li, Jiang
Li, Xiaomeng
author_sort Razzaq, Aroona
collection PubMed
description BACKGROUND: Peripheral nerve injury is a debilitating condition that may lead to partial or complete motor, sensory and autonomic function loss and lacks effective therapy until date. Therefore, it is quite imperative to explore impending remedies for rapid and accurate functional retrieval following such conditions. Natural product-based intervention can prove effective to facilitate the process of functions regain. METHODS: Here, we investigated the effect of processed Strychnos nux-vomica seeds at a dose of 250 mg/kg body weight in a mouse model of induced Sciatic nerve lesion in promoting the recovery of the functions. A compression injury was induced in the Sciatic nerve of the right leg in the mice. Sensory function recovery was evaluated by hot-plate and formalin tests, whereas the motor function retrieval was assessed by measuring muscle grip strength, sciatic functional index, and muscle mass restoration. Oxidative stress and blood cell count were measured by biochemistry and haematological analyses. RESULTS: This study indicates that Strychnos nux-vomica seeds enhance the rate of recovery of both sensory and motor functions. It helps restore the muscle mass, attenuates total oxidant status and enhances the total anti-oxidant capacity of the biological system. Moreover, the treated animals manifested an enhanced glucose tolerance aptitude and augmented granulocyte and platelet counts. Improved oxidant control, enhanced glucose sensitivity and amended granulocyte and platelet counts are likely to contribute to the advantageous effects of Strychnos nux-vomica, and warrant further in-depth studies for deciphering possible mechanisms and identification of active constituent(s) responsible for these effects. CONCLUSION: Strychnos nux-vomica seed offers functional recovery promoting effects following a mechanical injury to the Sciatic nerve and the possible reasons behind this effect can be reduced oxidative stress and improved glycaemic control. Further and detailed investigations can unravel this mystery.
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spelling pubmed-72916322020-06-12 Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury Razzaq, Aroona Hussain, Ghulam Rasul, Azhar Xu, Jiaqi Zhang, Qiqi Malik, Shoaib Ahmad Anwar, Haseeb Aziz, Nimra Braidy, Nady de Aguilar, Jose-Luis Gonzalez Wei, Wei Li, Jiang Li, Xiaomeng BMC Complement Med Ther Research Article BACKGROUND: Peripheral nerve injury is a debilitating condition that may lead to partial or complete motor, sensory and autonomic function loss and lacks effective therapy until date. Therefore, it is quite imperative to explore impending remedies for rapid and accurate functional retrieval following such conditions. Natural product-based intervention can prove effective to facilitate the process of functions regain. METHODS: Here, we investigated the effect of processed Strychnos nux-vomica seeds at a dose of 250 mg/kg body weight in a mouse model of induced Sciatic nerve lesion in promoting the recovery of the functions. A compression injury was induced in the Sciatic nerve of the right leg in the mice. Sensory function recovery was evaluated by hot-plate and formalin tests, whereas the motor function retrieval was assessed by measuring muscle grip strength, sciatic functional index, and muscle mass restoration. Oxidative stress and blood cell count were measured by biochemistry and haematological analyses. RESULTS: This study indicates that Strychnos nux-vomica seeds enhance the rate of recovery of both sensory and motor functions. It helps restore the muscle mass, attenuates total oxidant status and enhances the total anti-oxidant capacity of the biological system. Moreover, the treated animals manifested an enhanced glucose tolerance aptitude and augmented granulocyte and platelet counts. Improved oxidant control, enhanced glucose sensitivity and amended granulocyte and platelet counts are likely to contribute to the advantageous effects of Strychnos nux-vomica, and warrant further in-depth studies for deciphering possible mechanisms and identification of active constituent(s) responsible for these effects. CONCLUSION: Strychnos nux-vomica seed offers functional recovery promoting effects following a mechanical injury to the Sciatic nerve and the possible reasons behind this effect can be reduced oxidative stress and improved glycaemic control. Further and detailed investigations can unravel this mystery. BioMed Central 2020-06-11 /pmc/articles/PMC7291632/ /pubmed/32527244 http://dx.doi.org/10.1186/s12906-020-02950-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Razzaq, Aroona
Hussain, Ghulam
Rasul, Azhar
Xu, Jiaqi
Zhang, Qiqi
Malik, Shoaib Ahmad
Anwar, Haseeb
Aziz, Nimra
Braidy, Nady
de Aguilar, Jose-Luis Gonzalez
Wei, Wei
Li, Jiang
Li, Xiaomeng
Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury
title Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury
title_full Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury
title_fullStr Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury
title_full_unstemmed Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury
title_short Strychnos nux-vomica L. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury
title_sort strychnos nux-vomica l. seed preparation promotes functional recovery and attenuates oxidative stress in a mouse model of sciatic nerve crush injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291632/
https://www.ncbi.nlm.nih.gov/pubmed/32527244
http://dx.doi.org/10.1186/s12906-020-02950-3
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