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Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing
In this work, we report 2 cases of vancomycin-resistant Enterococcus faecium bacteremia with development of daptomycin resistance in 2 patients with acute myeloid leukemia and myelodysplastic syndrome. Mutations related to daptomycin-nonsusceptible phenotype in liaSR genes were found in all strains...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291683/ https://www.ncbi.nlm.nih.gov/pubmed/32550237 http://dx.doi.org/10.1093/ofid/ofaa180 |
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author | Udaondo, Zulema Jenjaroenpun, Piroon Wongsurawat, Thidathip Meyers, Erik Anderson, Courtney Lopez, James Mohan, Meera Tytarenko, Ruslana Walker, Brian Ussery, David Kothari, Atul Jun, Se-Ran |
author_facet | Udaondo, Zulema Jenjaroenpun, Piroon Wongsurawat, Thidathip Meyers, Erik Anderson, Courtney Lopez, James Mohan, Meera Tytarenko, Ruslana Walker, Brian Ussery, David Kothari, Atul Jun, Se-Ran |
author_sort | Udaondo, Zulema |
collection | PubMed |
description | In this work, we report 2 cases of vancomycin-resistant Enterococcus faecium bacteremia with development of daptomycin resistance in 2 patients with acute myeloid leukemia and myelodysplastic syndrome. Mutations related to daptomycin-nonsusceptible phenotype in liaSR genes were found in all strains of the study, including those with a minimum inhibitory concentration <1 µg/mL collected before daptomycin therapy. Epidemiological investigation using core genome single nucleotide polymorphism and core genome multilocus sequence typing revealed clonality of all the isolates. In this study, we conclude that real-time genome sequencing of clinical isolates can provide rapid access to timely information on daptomycin-resistant genotypes that would help clinicians speed up and optimize the selection of the antibiotic for treatment. |
format | Online Article Text |
id | pubmed-7291683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72916832020-06-16 Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing Udaondo, Zulema Jenjaroenpun, Piroon Wongsurawat, Thidathip Meyers, Erik Anderson, Courtney Lopez, James Mohan, Meera Tytarenko, Ruslana Walker, Brian Ussery, David Kothari, Atul Jun, Se-Ran Open Forum Infect Dis ID Cases In this work, we report 2 cases of vancomycin-resistant Enterococcus faecium bacteremia with development of daptomycin resistance in 2 patients with acute myeloid leukemia and myelodysplastic syndrome. Mutations related to daptomycin-nonsusceptible phenotype in liaSR genes were found in all strains of the study, including those with a minimum inhibitory concentration <1 µg/mL collected before daptomycin therapy. Epidemiological investigation using core genome single nucleotide polymorphism and core genome multilocus sequence typing revealed clonality of all the isolates. In this study, we conclude that real-time genome sequencing of clinical isolates can provide rapid access to timely information on daptomycin-resistant genotypes that would help clinicians speed up and optimize the selection of the antibiotic for treatment. Oxford University Press 2020-05-23 /pmc/articles/PMC7291683/ /pubmed/32550237 http://dx.doi.org/10.1093/ofid/ofaa180 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | ID Cases Udaondo, Zulema Jenjaroenpun, Piroon Wongsurawat, Thidathip Meyers, Erik Anderson, Courtney Lopez, James Mohan, Meera Tytarenko, Ruslana Walker, Brian Ussery, David Kothari, Atul Jun, Se-Ran Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing |
title | Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing |
title_full | Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing |
title_fullStr | Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing |
title_full_unstemmed | Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing |
title_short | Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing |
title_sort | two cases of vancomycin-resistant enterococcus faecium bacteremia with development of daptomycin-resistant phenotype and its detection using oxford nanopore sequencing |
topic | ID Cases |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291683/ https://www.ncbi.nlm.nih.gov/pubmed/32550237 http://dx.doi.org/10.1093/ofid/ofaa180 |
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