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Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing

In this work, we report 2 cases of vancomycin-resistant Enterococcus faecium bacteremia with development of daptomycin resistance in 2 patients with acute myeloid leukemia and myelodysplastic syndrome. Mutations related to daptomycin-nonsusceptible phenotype in liaSR genes were found in all strains...

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Autores principales: Udaondo, Zulema, Jenjaroenpun, Piroon, Wongsurawat, Thidathip, Meyers, Erik, Anderson, Courtney, Lopez, James, Mohan, Meera, Tytarenko, Ruslana, Walker, Brian, Ussery, David, Kothari, Atul, Jun, Se-Ran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291683/
https://www.ncbi.nlm.nih.gov/pubmed/32550237
http://dx.doi.org/10.1093/ofid/ofaa180
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author Udaondo, Zulema
Jenjaroenpun, Piroon
Wongsurawat, Thidathip
Meyers, Erik
Anderson, Courtney
Lopez, James
Mohan, Meera
Tytarenko, Ruslana
Walker, Brian
Ussery, David
Kothari, Atul
Jun, Se-Ran
author_facet Udaondo, Zulema
Jenjaroenpun, Piroon
Wongsurawat, Thidathip
Meyers, Erik
Anderson, Courtney
Lopez, James
Mohan, Meera
Tytarenko, Ruslana
Walker, Brian
Ussery, David
Kothari, Atul
Jun, Se-Ran
author_sort Udaondo, Zulema
collection PubMed
description In this work, we report 2 cases of vancomycin-resistant Enterococcus faecium bacteremia with development of daptomycin resistance in 2 patients with acute myeloid leukemia and myelodysplastic syndrome. Mutations related to daptomycin-nonsusceptible phenotype in liaSR genes were found in all strains of the study, including those with a minimum inhibitory concentration <1 µg/mL collected before daptomycin therapy. Epidemiological investigation using core genome single nucleotide polymorphism and core genome multilocus sequence typing revealed clonality of all the isolates. In this study, we conclude that real-time genome sequencing of clinical isolates can provide rapid access to timely information on daptomycin-resistant genotypes that would help clinicians speed up and optimize the selection of the antibiotic for treatment.
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spelling pubmed-72916832020-06-16 Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing Udaondo, Zulema Jenjaroenpun, Piroon Wongsurawat, Thidathip Meyers, Erik Anderson, Courtney Lopez, James Mohan, Meera Tytarenko, Ruslana Walker, Brian Ussery, David Kothari, Atul Jun, Se-Ran Open Forum Infect Dis ID Cases In this work, we report 2 cases of vancomycin-resistant Enterococcus faecium bacteremia with development of daptomycin resistance in 2 patients with acute myeloid leukemia and myelodysplastic syndrome. Mutations related to daptomycin-nonsusceptible phenotype in liaSR genes were found in all strains of the study, including those with a minimum inhibitory concentration <1 µg/mL collected before daptomycin therapy. Epidemiological investigation using core genome single nucleotide polymorphism and core genome multilocus sequence typing revealed clonality of all the isolates. In this study, we conclude that real-time genome sequencing of clinical isolates can provide rapid access to timely information on daptomycin-resistant genotypes that would help clinicians speed up and optimize the selection of the antibiotic for treatment. Oxford University Press 2020-05-23 /pmc/articles/PMC7291683/ /pubmed/32550237 http://dx.doi.org/10.1093/ofid/ofaa180 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle ID Cases
Udaondo, Zulema
Jenjaroenpun, Piroon
Wongsurawat, Thidathip
Meyers, Erik
Anderson, Courtney
Lopez, James
Mohan, Meera
Tytarenko, Ruslana
Walker, Brian
Ussery, David
Kothari, Atul
Jun, Se-Ran
Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing
title Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing
title_full Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing
title_fullStr Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing
title_full_unstemmed Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing
title_short Two Cases of Vancomycin-Resistant Enterococcus faecium Bacteremia With Development of Daptomycin-Resistant Phenotype and its Detection Using Oxford Nanopore Sequencing
title_sort two cases of vancomycin-resistant enterococcus faecium bacteremia with development of daptomycin-resistant phenotype and its detection using oxford nanopore sequencing
topic ID Cases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291683/
https://www.ncbi.nlm.nih.gov/pubmed/32550237
http://dx.doi.org/10.1093/ofid/ofaa180
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