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Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes
A global emergency due to the COVID-19 pandemic demands various studies related to genes and genomes of the SARS-CoV2. Among other important proteins, the role of accessory proteins are of immense importance in replication, regulation of infections of the coronavirus in the hosts. The largest access...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291963/ https://www.ncbi.nlm.nih.gov/pubmed/32540495 http://dx.doi.org/10.1016/j.ygeno.2020.06.016 |
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author | Hassan, Sk. Sarif Choudhury, Pabitra Pal Basu, Pallab Jana, Siddhartha Sankar |
author_facet | Hassan, Sk. Sarif Choudhury, Pabitra Pal Basu, Pallab Jana, Siddhartha Sankar |
author_sort | Hassan, Sk. Sarif |
collection | PubMed |
description | A global emergency due to the COVID-19 pandemic demands various studies related to genes and genomes of the SARS-CoV2. Among other important proteins, the role of accessory proteins are of immense importance in replication, regulation of infections of the coronavirus in the hosts. The largest accessory protein in the SARS-CoV2 genome is ORF3a which modulates the host response to the virus infection and consequently it plays an important role in pathogenesis. In this study, an attempt is made to decipher the conservation of nucleotides, dimers, codons and amino acids in the ORF3a genes across thirty-two genomes of Indian patients. ORF3a gene possesses single and double point mutations in Indian SARS-CoV2 genomes suggesting the change of SARS-CoV2's virulence property in Indian patients. We find that the parental origin of the ORF3a gene over the genomes of SARS-CoV2 and Pangolin-CoV is same from the phylogenetic analysis based on conservation of nucleotides and so on. This study highlights the accumulation of mutation on ORF3a in Indian SARS-CoV2 genomes which may provide the designing therapeutic approach against SARS-CoV2. |
format | Online Article Text |
id | pubmed-7291963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72919632020-06-12 Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes Hassan, Sk. Sarif Choudhury, Pabitra Pal Basu, Pallab Jana, Siddhartha Sankar Genomics Article A global emergency due to the COVID-19 pandemic demands various studies related to genes and genomes of the SARS-CoV2. Among other important proteins, the role of accessory proteins are of immense importance in replication, regulation of infections of the coronavirus in the hosts. The largest accessory protein in the SARS-CoV2 genome is ORF3a which modulates the host response to the virus infection and consequently it plays an important role in pathogenesis. In this study, an attempt is made to decipher the conservation of nucleotides, dimers, codons and amino acids in the ORF3a genes across thirty-two genomes of Indian patients. ORF3a gene possesses single and double point mutations in Indian SARS-CoV2 genomes suggesting the change of SARS-CoV2's virulence property in Indian patients. We find that the parental origin of the ORF3a gene over the genomes of SARS-CoV2 and Pangolin-CoV is same from the phylogenetic analysis based on conservation of nucleotides and so on. This study highlights the accumulation of mutation on ORF3a in Indian SARS-CoV2 genomes which may provide the designing therapeutic approach against SARS-CoV2. Elsevier Inc. 2020-09 2020-06-12 /pmc/articles/PMC7291963/ /pubmed/32540495 http://dx.doi.org/10.1016/j.ygeno.2020.06.016 Text en © 2020 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Hassan, Sk. Sarif Choudhury, Pabitra Pal Basu, Pallab Jana, Siddhartha Sankar Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes |
title | Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes |
title_full | Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes |
title_fullStr | Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes |
title_full_unstemmed | Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes |
title_short | Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes |
title_sort | molecular conservation and differential mutation on orf3a gene in indian sars-cov2 genomes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291963/ https://www.ncbi.nlm.nih.gov/pubmed/32540495 http://dx.doi.org/10.1016/j.ygeno.2020.06.016 |
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