Cargando…
Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder
Amino acid neurotransmitters have been shown to correlate with Tourette syndrome (TS) and its comorbidities. In this study, we investigated the effects of Jian-Pi-Zhi-Dong Decoction (JPZDD), a formula containing 10 different Chinese medical herbs, on amino acid neurotransmitters in rats. We establis...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292006/ https://www.ncbi.nlm.nih.gov/pubmed/32581885 http://dx.doi.org/10.3389/fpsyt.2020.00515 |
_version_ | 1783546016080855040 |
---|---|
author | Zhang, Wen Yu, Wenjing Liu, Xiaofang Wang, Qian Bai, Xue Cui, Xia Wang, Sumei |
author_facet | Zhang, Wen Yu, Wenjing Liu, Xiaofang Wang, Qian Bai, Xue Cui, Xia Wang, Sumei |
author_sort | Zhang, Wen |
collection | PubMed |
description | Amino acid neurotransmitters have been shown to correlate with Tourette syndrome (TS) and its comorbidities. In this study, we investigated the effects of Jian-Pi-Zhi-Dong Decoction (JPZDD), a formula containing 10 different Chinese medical herbs, on amino acid neurotransmitters in rats. We established a rat model of Tourette syndrome and comorbid anxiety with an iminodipropionitrile injection plus uncertain empty water bottle stimulation for 3 weeks. Then the rats were randomly divided into four groups: control group and model group were gavaged with saline, while the remaining two treatment groups were gavaged with fluoxetine hydrochloride or JPZDD for four consecutive weeks. We recorded the behaviors of the rats with TS and comorbid anxiety by stereotypy recording, open field test, and elevated plus maze. We observed mitochondrial changes with transmission electron microscopy. We measured the content of glutamate (GLU) and γ-aminobutyric acid (GABA) both in the serum and striatum and the expression of their receptors by Western blot and real-time polymerase chain reaction. The study revealed that JPZDD was effective in alleviating the behavioral symptoms of both tic and anxiety in the rat model groups. These results might be associated with the increase in GABA levels and decrease in GLU levels in the serum, as well as an increase in striatal GABA level by the activation of GABA receptors Type A (GABAAR). JPZDD treatment also reversed the mitochondrial dysfunction both in the striatum and cortex in affected animals. |
format | Online Article Text |
id | pubmed-7292006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72920062020-06-23 Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder Zhang, Wen Yu, Wenjing Liu, Xiaofang Wang, Qian Bai, Xue Cui, Xia Wang, Sumei Front Psychiatry Psychiatry Amino acid neurotransmitters have been shown to correlate with Tourette syndrome (TS) and its comorbidities. In this study, we investigated the effects of Jian-Pi-Zhi-Dong Decoction (JPZDD), a formula containing 10 different Chinese medical herbs, on amino acid neurotransmitters in rats. We established a rat model of Tourette syndrome and comorbid anxiety with an iminodipropionitrile injection plus uncertain empty water bottle stimulation for 3 weeks. Then the rats were randomly divided into four groups: control group and model group were gavaged with saline, while the remaining two treatment groups were gavaged with fluoxetine hydrochloride or JPZDD for four consecutive weeks. We recorded the behaviors of the rats with TS and comorbid anxiety by stereotypy recording, open field test, and elevated plus maze. We observed mitochondrial changes with transmission electron microscopy. We measured the content of glutamate (GLU) and γ-aminobutyric acid (GABA) both in the serum and striatum and the expression of their receptors by Western blot and real-time polymerase chain reaction. The study revealed that JPZDD was effective in alleviating the behavioral symptoms of both tic and anxiety in the rat model groups. These results might be associated with the increase in GABA levels and decrease in GLU levels in the serum, as well as an increase in striatal GABA level by the activation of GABA receptors Type A (GABAAR). JPZDD treatment also reversed the mitochondrial dysfunction both in the striatum and cortex in affected animals. Frontiers Media S.A. 2020-06-05 /pmc/articles/PMC7292006/ /pubmed/32581885 http://dx.doi.org/10.3389/fpsyt.2020.00515 Text en Copyright © 2020 Zhang, Yu, Liu, Wang, Bai, Cui and Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Psychiatry Zhang, Wen Yu, Wenjing Liu, Xiaofang Wang, Qian Bai, Xue Cui, Xia Wang, Sumei Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder |
title | Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder |
title_full | Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder |
title_fullStr | Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder |
title_full_unstemmed | Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder |
title_short | Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder |
title_sort | effect of jian-pi-zhi-dong decoction on the amino acid neurotransmitters in a rat model of tourette syndrome and comorbid anxiety disorder |
topic | Psychiatry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292006/ https://www.ncbi.nlm.nih.gov/pubmed/32581885 http://dx.doi.org/10.3389/fpsyt.2020.00515 |
work_keys_str_mv | AT zhangwen effectofjianpizhidongdecoctionontheaminoacidneurotransmittersinaratmodeloftourettesyndromeandcomorbidanxietydisorder AT yuwenjing effectofjianpizhidongdecoctionontheaminoacidneurotransmittersinaratmodeloftourettesyndromeandcomorbidanxietydisorder AT liuxiaofang effectofjianpizhidongdecoctionontheaminoacidneurotransmittersinaratmodeloftourettesyndromeandcomorbidanxietydisorder AT wangqian effectofjianpizhidongdecoctionontheaminoacidneurotransmittersinaratmodeloftourettesyndromeandcomorbidanxietydisorder AT baixue effectofjianpizhidongdecoctionontheaminoacidneurotransmittersinaratmodeloftourettesyndromeandcomorbidanxietydisorder AT cuixia effectofjianpizhidongdecoctionontheaminoacidneurotransmittersinaratmodeloftourettesyndromeandcomorbidanxietydisorder AT wangsumei effectofjianpizhidongdecoctionontheaminoacidneurotransmittersinaratmodeloftourettesyndromeandcomorbidanxietydisorder |