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Is NAT2 Gene Polymorphism Associated with Vitiligo?

BACKGROUND: N-acetyltransferase-2 (NAT2) is a phase II xenobiotic enzyme that plays an important role against oxidative stress-mediated reactive oxygen species protection. Polymorphism in specific genotypes of NAT2 may lead to increase an imbalance in antioxidant systems and may influence the pathog...

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Autores principales: Srivastava, Daya Shankar Lal, Aggarwal, Kamal, Singh, Gajendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292445/
https://www.ncbi.nlm.nih.gov/pubmed/32565555
http://dx.doi.org/10.4103/ijd.IJD_388_18
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author Srivastava, Daya Shankar Lal
Aggarwal, Kamal
Singh, Gajendra
author_facet Srivastava, Daya Shankar Lal
Aggarwal, Kamal
Singh, Gajendra
author_sort Srivastava, Daya Shankar Lal
collection PubMed
description BACKGROUND: N-acetyltransferase-2 (NAT2) is a phase II xenobiotic enzyme that plays an important role against oxidative stress-mediated reactive oxygen species protection. Polymorphism in specific genotypes of NAT2 may lead to increase an imbalance in antioxidant systems and may influence the pathogenesis of vitiligo. We conducted this study to see the association between NAT2 gene polymorphism and risk of vitiligo. We looked into whether single-nucleotide polymorphisms (SNP) at positions 857, 481 and 590 of the coding region of the NAT2 gene play as a risk factor for vitiligo among north Indian people. METHODS: In this study, we assessed 100 patients with vitiligo and 160 healthy individuals as controls. Genomic DNA was extracted from human peripheral blood and polymerase chain reaction–restricted fragment length polymorphism was done to identify the single nucleotide polymorphism at positions 857, 481, and 590 of the coding region of the NAT2 gene. RESULTS: In this study, we observed a significant higher risk with slow acetylator genotypes of NAT2 (OR = 2.85; 95% CI = 1.68-4.84, P value < 0.001) for the vitiligo. Furthermore, in the association between NAT2 acetylator genotypes with percentage of body surface area (BSA) of disease, we observed that slow acetylator genotypes of NAT2 has significant higher risk with low grade of disease (1%–10% >11%–30% >30% of BSA). LIMITATIONS: A major limitation of this study was the small sample size and warrants further investigation on a large epidemiological study to confirm these findings. CONCLUSIONS: Our preliminary data indicate that NAT2 slow acetylator genotype exhibits significant association for the risk of vitiligo, especially in disease predisposition and initiation.
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spelling pubmed-72924452020-06-19 Is NAT2 Gene Polymorphism Associated with Vitiligo? Srivastava, Daya Shankar Lal Aggarwal, Kamal Singh, Gajendra Indian J Dermatol Basic Research BACKGROUND: N-acetyltransferase-2 (NAT2) is a phase II xenobiotic enzyme that plays an important role against oxidative stress-mediated reactive oxygen species protection. Polymorphism in specific genotypes of NAT2 may lead to increase an imbalance in antioxidant systems and may influence the pathogenesis of vitiligo. We conducted this study to see the association between NAT2 gene polymorphism and risk of vitiligo. We looked into whether single-nucleotide polymorphisms (SNP) at positions 857, 481 and 590 of the coding region of the NAT2 gene play as a risk factor for vitiligo among north Indian people. METHODS: In this study, we assessed 100 patients with vitiligo and 160 healthy individuals as controls. Genomic DNA was extracted from human peripheral blood and polymerase chain reaction–restricted fragment length polymorphism was done to identify the single nucleotide polymorphism at positions 857, 481, and 590 of the coding region of the NAT2 gene. RESULTS: In this study, we observed a significant higher risk with slow acetylator genotypes of NAT2 (OR = 2.85; 95% CI = 1.68-4.84, P value < 0.001) for the vitiligo. Furthermore, in the association between NAT2 acetylator genotypes with percentage of body surface area (BSA) of disease, we observed that slow acetylator genotypes of NAT2 has significant higher risk with low grade of disease (1%–10% >11%–30% >30% of BSA). LIMITATIONS: A major limitation of this study was the small sample size and warrants further investigation on a large epidemiological study to confirm these findings. CONCLUSIONS: Our preliminary data indicate that NAT2 slow acetylator genotype exhibits significant association for the risk of vitiligo, especially in disease predisposition and initiation. Wolters Kluwer - Medknow 2020 /pmc/articles/PMC7292445/ /pubmed/32565555 http://dx.doi.org/10.4103/ijd.IJD_388_18 Text en Copyright: © 2020 Indian Journal of Dermatology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Basic Research
Srivastava, Daya Shankar Lal
Aggarwal, Kamal
Singh, Gajendra
Is NAT2 Gene Polymorphism Associated with Vitiligo?
title Is NAT2 Gene Polymorphism Associated with Vitiligo?
title_full Is NAT2 Gene Polymorphism Associated with Vitiligo?
title_fullStr Is NAT2 Gene Polymorphism Associated with Vitiligo?
title_full_unstemmed Is NAT2 Gene Polymorphism Associated with Vitiligo?
title_short Is NAT2 Gene Polymorphism Associated with Vitiligo?
title_sort is nat2 gene polymorphism associated with vitiligo?
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292445/
https://www.ncbi.nlm.nih.gov/pubmed/32565555
http://dx.doi.org/10.4103/ijd.IJD_388_18
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