Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins

We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning–based software...

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Detalles Bibliográficos
Autores principales: Mishra, Kanuj, Stankevych, Mariia, Fuenzalida-Werner, Juan Pablo, Grassmann, Simon, Gujrati, Vipul, Huang, Yuanhui, Klemm, Uwe, Buchholz, Veit R., Ntziachristos, Vasilis, Stiel, Andre C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292636/
https://www.ncbi.nlm.nih.gov/pubmed/32582850
http://dx.doi.org/10.1126/sciadv.aaz6293
Descripción
Sumario:We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning–based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.

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