Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins

We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning–based software...

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Detalles Bibliográficos
Autores principales: Mishra, Kanuj, Stankevych, Mariia, Fuenzalida-Werner, Juan Pablo, Grassmann, Simon, Gujrati, Vipul, Huang, Yuanhui, Klemm, Uwe, Buchholz, Veit R., Ntziachristos, Vasilis, Stiel, Andre C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292636/
https://www.ncbi.nlm.nih.gov/pubmed/32582850
http://dx.doi.org/10.1126/sciadv.aaz6293
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author Mishra, Kanuj
Stankevych, Mariia
Fuenzalida-Werner, Juan Pablo
Grassmann, Simon
Gujrati, Vipul
Huang, Yuanhui
Klemm, Uwe
Buchholz, Veit R.
Ntziachristos, Vasilis
Stiel, Andre C.
author_facet Mishra, Kanuj
Stankevych, Mariia
Fuenzalida-Werner, Juan Pablo
Grassmann, Simon
Gujrati, Vipul
Huang, Yuanhui
Klemm, Uwe
Buchholz, Veit R.
Ntziachristos, Vasilis
Stiel, Andre C.
author_sort Mishra, Kanuj
collection PubMed
description We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning–based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.
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spelling pubmed-72926362020-06-23 Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins Mishra, Kanuj Stankevych, Mariia Fuenzalida-Werner, Juan Pablo Grassmann, Simon Gujrati, Vipul Huang, Yuanhui Klemm, Uwe Buchholz, Veit R. Ntziachristos, Vasilis Stiel, Andre C. Sci Adv Research Articles We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning–based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences. American Association for the Advancement of Science 2020-06-12 /pmc/articles/PMC7292636/ /pubmed/32582850 http://dx.doi.org/10.1126/sciadv.aaz6293 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Mishra, Kanuj
Stankevych, Mariia
Fuenzalida-Werner, Juan Pablo
Grassmann, Simon
Gujrati, Vipul
Huang, Yuanhui
Klemm, Uwe
Buchholz, Veit R.
Ntziachristos, Vasilis
Stiel, Andre C.
Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
title Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
title_full Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
title_fullStr Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
title_full_unstemmed Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
title_short Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
title_sort multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292636/
https://www.ncbi.nlm.nih.gov/pubmed/32582850
http://dx.doi.org/10.1126/sciadv.aaz6293
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