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Encoding multiple digital DNA signals in a single analog channel
For many analytic and biomedical applications, the presence of an analyte above or below a critical concentration is more informative for decision making than the actual concentration value. Straightforward analog-to-digital signal conversion does not take full advantage of the precision and dynamic...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293012/ https://www.ncbi.nlm.nih.gov/pubmed/32338742 http://dx.doi.org/10.1093/nar/gkaa303 |
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author | Yan, Yan Helen Zhang, David Yu Wu, Lucia R |
author_facet | Yan, Yan Helen Zhang, David Yu Wu, Lucia R |
author_sort | Yan, Yan Helen |
collection | PubMed |
description | For many analytic and biomedical applications, the presence of an analyte above or below a critical concentration is more informative for decision making than the actual concentration value. Straightforward analog-to-digital signal conversion does not take full advantage of the precision and dynamic range of modern sensors. Here, we present and experimentally demonstrate an analog-to-multiple-digital signal conversion, reporting digital signals that indicate whether the concentrations of specific DNA sequences exceed respective threshold values. These threshold values can be individually programmed for each target sequence. Experimentally, we showed representation of four DNA targets’ information in a single fluorescence channel. |
format | Online Article Text |
id | pubmed-7293012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72930122020-06-17 Encoding multiple digital DNA signals in a single analog channel Yan, Yan Helen Zhang, David Yu Wu, Lucia R Nucleic Acids Res Methods Online For many analytic and biomedical applications, the presence of an analyte above or below a critical concentration is more informative for decision making than the actual concentration value. Straightforward analog-to-digital signal conversion does not take full advantage of the precision and dynamic range of modern sensors. Here, we present and experimentally demonstrate an analog-to-multiple-digital signal conversion, reporting digital signals that indicate whether the concentrations of specific DNA sequences exceed respective threshold values. These threshold values can be individually programmed for each target sequence. Experimentally, we showed representation of four DNA targets’ information in a single fluorescence channel. Oxford University Press 2020-06-19 2020-04-27 /pmc/articles/PMC7293012/ /pubmed/32338742 http://dx.doi.org/10.1093/nar/gkaa303 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Methods Online Yan, Yan Helen Zhang, David Yu Wu, Lucia R Encoding multiple digital DNA signals in a single analog channel |
title | Encoding multiple digital DNA signals in a single analog channel |
title_full | Encoding multiple digital DNA signals in a single analog channel |
title_fullStr | Encoding multiple digital DNA signals in a single analog channel |
title_full_unstemmed | Encoding multiple digital DNA signals in a single analog channel |
title_short | Encoding multiple digital DNA signals in a single analog channel |
title_sort | encoding multiple digital dna signals in a single analog channel |
topic | Methods Online |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293012/ https://www.ncbi.nlm.nih.gov/pubmed/32338742 http://dx.doi.org/10.1093/nar/gkaa303 |
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