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Encoding multiple digital DNA signals in a single analog channel

For many analytic and biomedical applications, the presence of an analyte above or below a critical concentration is more informative for decision making than the actual concentration value. Straightforward analog-to-digital signal conversion does not take full advantage of the precision and dynamic...

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Detalles Bibliográficos
Autores principales: Yan, Yan Helen, Zhang, David Yu, Wu, Lucia R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293012/
https://www.ncbi.nlm.nih.gov/pubmed/32338742
http://dx.doi.org/10.1093/nar/gkaa303
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author Yan, Yan Helen
Zhang, David Yu
Wu, Lucia R
author_facet Yan, Yan Helen
Zhang, David Yu
Wu, Lucia R
author_sort Yan, Yan Helen
collection PubMed
description For many analytic and biomedical applications, the presence of an analyte above or below a critical concentration is more informative for decision making than the actual concentration value. Straightforward analog-to-digital signal conversion does not take full advantage of the precision and dynamic range of modern sensors. Here, we present and experimentally demonstrate an analog-to-multiple-digital signal conversion, reporting digital signals that indicate whether the concentrations of specific DNA sequences exceed respective threshold values. These threshold values can be individually programmed for each target sequence. Experimentally, we showed representation of four DNA targets’ information in a single fluorescence channel.
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spelling pubmed-72930122020-06-17 Encoding multiple digital DNA signals in a single analog channel Yan, Yan Helen Zhang, David Yu Wu, Lucia R Nucleic Acids Res Methods Online For many analytic and biomedical applications, the presence of an analyte above or below a critical concentration is more informative for decision making than the actual concentration value. Straightforward analog-to-digital signal conversion does not take full advantage of the precision and dynamic range of modern sensors. Here, we present and experimentally demonstrate an analog-to-multiple-digital signal conversion, reporting digital signals that indicate whether the concentrations of specific DNA sequences exceed respective threshold values. These threshold values can be individually programmed for each target sequence. Experimentally, we showed representation of four DNA targets’ information in a single fluorescence channel. Oxford University Press 2020-06-19 2020-04-27 /pmc/articles/PMC7293012/ /pubmed/32338742 http://dx.doi.org/10.1093/nar/gkaa303 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Methods Online
Yan, Yan Helen
Zhang, David Yu
Wu, Lucia R
Encoding multiple digital DNA signals in a single analog channel
title Encoding multiple digital DNA signals in a single analog channel
title_full Encoding multiple digital DNA signals in a single analog channel
title_fullStr Encoding multiple digital DNA signals in a single analog channel
title_full_unstemmed Encoding multiple digital DNA signals in a single analog channel
title_short Encoding multiple digital DNA signals in a single analog channel
title_sort encoding multiple digital dna signals in a single analog channel
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293012/
https://www.ncbi.nlm.nih.gov/pubmed/32338742
http://dx.doi.org/10.1093/nar/gkaa303
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