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Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling

Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish...

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Autores principales: Safarian, Nickie, Whyte-Fagundes, Paige, Zoidl, Christiane, Grigull, Jörg, Zoidl, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293225/
https://www.ncbi.nlm.nih.gov/pubmed/32533080
http://dx.doi.org/10.1038/s41598-020-66378-y
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author Safarian, Nickie
Whyte-Fagundes, Paige
Zoidl, Christiane
Grigull, Jörg
Zoidl, Georg
author_facet Safarian, Nickie
Whyte-Fagundes, Paige
Zoidl, Christiane
Grigull, Jörg
Zoidl, Georg
author_sort Safarian, Nickie
collection PubMed
description Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish panx1a gene using TALEN technology. Here, RNA-seq analysis of 6 days post fertilization larvae were confirmed by Real-Time PCR and paired with testing visual-motor behavior and in vivo electrophysiology. Results demonstrated that loss of panx1a specifically affected the expression of gene classes representing the development of the visual system and visual processing. Abnormal swimming behavior in the dark and the expression regulation of pre-and postsynaptic biomarkers suggested changes in dopaminergic signaling. Indeed, altered visuomotor behavior in the absence of functional Panx1a was evoked through D1/D2-like receptor agonist treatment and rescued with the D2-like receptor antagonist Haloperidol. Local field potentials recorded from superficial areas of the optic tectum receiving input from the retina confirmed abnormal responses to visual stimuli, which resembled treatments with a dopamine receptor agonist or pharmacological blocking of Panx1a. We conclude that Panx1a functions are relevant at a time point when neuronal networks supporting visual-motor functions undergo modifications preparing for complex behaviors of freely swimming fish.
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spelling pubmed-72932252020-06-15 Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling Safarian, Nickie Whyte-Fagundes, Paige Zoidl, Christiane Grigull, Jörg Zoidl, Georg Sci Rep Article Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish panx1a gene using TALEN technology. Here, RNA-seq analysis of 6 days post fertilization larvae were confirmed by Real-Time PCR and paired with testing visual-motor behavior and in vivo electrophysiology. Results demonstrated that loss of panx1a specifically affected the expression of gene classes representing the development of the visual system and visual processing. Abnormal swimming behavior in the dark and the expression regulation of pre-and postsynaptic biomarkers suggested changes in dopaminergic signaling. Indeed, altered visuomotor behavior in the absence of functional Panx1a was evoked through D1/D2-like receptor agonist treatment and rescued with the D2-like receptor antagonist Haloperidol. Local field potentials recorded from superficial areas of the optic tectum receiving input from the retina confirmed abnormal responses to visual stimuli, which resembled treatments with a dopamine receptor agonist or pharmacological blocking of Panx1a. We conclude that Panx1a functions are relevant at a time point when neuronal networks supporting visual-motor functions undergo modifications preparing for complex behaviors of freely swimming fish. Nature Publishing Group UK 2020-06-12 /pmc/articles/PMC7293225/ /pubmed/32533080 http://dx.doi.org/10.1038/s41598-020-66378-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Safarian, Nickie
Whyte-Fagundes, Paige
Zoidl, Christiane
Grigull, Jörg
Zoidl, Georg
Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
title Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
title_full Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
title_fullStr Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
title_full_unstemmed Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
title_short Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
title_sort visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293225/
https://www.ncbi.nlm.nih.gov/pubmed/32533080
http://dx.doi.org/10.1038/s41598-020-66378-y
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