Cargando…
Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches
Lipid oxidation and microbial contamination are the major factors contributing to food deterioration. Food additives like antioxidants and antibacterials can prevent food spoilage by delaying oxidation and preventing the growth of bacteria. Artocarpus altilis leaves exhibited biological properties t...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293230/ https://www.ncbi.nlm.nih.gov/pubmed/32533034 http://dx.doi.org/10.1038/s41598-020-66488-7 |
_version_ | 1783546257042571264 |
---|---|
author | Ahmad, Mohammad Norazmi Karim, Nazatul Umira Normaya, Erna Mat Piah, Bijarimi Iqbal, Anwar Ku Bulat, Ku Halim |
author_facet | Ahmad, Mohammad Norazmi Karim, Nazatul Umira Normaya, Erna Mat Piah, Bijarimi Iqbal, Anwar Ku Bulat, Ku Halim |
author_sort | Ahmad, Mohammad Norazmi |
collection | PubMed |
description | Lipid oxidation and microbial contamination are the major factors contributing to food deterioration. Food additives like antioxidants and antibacterials can prevent food spoilage by delaying oxidation and preventing the growth of bacteria. Artocarpus altilis leaves exhibited biological properties that suggested its use as a new source of natural antioxidant and antimicrobial. Supercritical fluid extraction (SFE) was used to optimize the extraction of bioactive compounds from the leaves using response surface methodology (yield and antioxidant activity). The optimum SFE conditions were 50.5 °C temperature, 3784 psi pressure and 52 min extraction time. Verification test results (Tukey’s test) showed that no significant difference between the expected and experimental DPPH activity and yield value (99%) were found. Gas-chromatography –mass spectrometry (GC-MS) analysis revealed three major bioactive compounds existed in A. altilis extract. The extract demonstrated antioxidant and antibacterial properties with 2,3-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, ferric reducing ability of plasma (FRAP), hydroxyl radical scavenging activity, tyrosinase mushrrom inhibition of 41.5%, 8.15 ± 1.31 (µg of ascorbic acid equivalents), 32%, 37% and inhibition zone diameter of 0.766 ± 0.06 cm (B. cereus) and 1.27 ± 0.12 cm (E. coli). Conductor like screening model for real solvents (COSMO RS) was performed to explain the extraction mechanism of the major bioactive compounds during SFE. Molecular electrostatic potential (MEP) shows the probability site of nucleophilic and electrophilic attack during bacterial inhibition. Based on molecular docking study, non-covalent interactions are the main interaction occurring between the major bioactive compounds and bacteria (antibacterial inhibition). |
format | Online Article Text |
id | pubmed-7293230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72932302020-06-15 Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches Ahmad, Mohammad Norazmi Karim, Nazatul Umira Normaya, Erna Mat Piah, Bijarimi Iqbal, Anwar Ku Bulat, Ku Halim Sci Rep Article Lipid oxidation and microbial contamination are the major factors contributing to food deterioration. Food additives like antioxidants and antibacterials can prevent food spoilage by delaying oxidation and preventing the growth of bacteria. Artocarpus altilis leaves exhibited biological properties that suggested its use as a new source of natural antioxidant and antimicrobial. Supercritical fluid extraction (SFE) was used to optimize the extraction of bioactive compounds from the leaves using response surface methodology (yield and antioxidant activity). The optimum SFE conditions were 50.5 °C temperature, 3784 psi pressure and 52 min extraction time. Verification test results (Tukey’s test) showed that no significant difference between the expected and experimental DPPH activity and yield value (99%) were found. Gas-chromatography –mass spectrometry (GC-MS) analysis revealed three major bioactive compounds existed in A. altilis extract. The extract demonstrated antioxidant and antibacterial properties with 2,3-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, ferric reducing ability of plasma (FRAP), hydroxyl radical scavenging activity, tyrosinase mushrrom inhibition of 41.5%, 8.15 ± 1.31 (µg of ascorbic acid equivalents), 32%, 37% and inhibition zone diameter of 0.766 ± 0.06 cm (B. cereus) and 1.27 ± 0.12 cm (E. coli). Conductor like screening model for real solvents (COSMO RS) was performed to explain the extraction mechanism of the major bioactive compounds during SFE. Molecular electrostatic potential (MEP) shows the probability site of nucleophilic and electrophilic attack during bacterial inhibition. Based on molecular docking study, non-covalent interactions are the main interaction occurring between the major bioactive compounds and bacteria (antibacterial inhibition). Nature Publishing Group UK 2020-06-12 /pmc/articles/PMC7293230/ /pubmed/32533034 http://dx.doi.org/10.1038/s41598-020-66488-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ahmad, Mohammad Norazmi Karim, Nazatul Umira Normaya, Erna Mat Piah, Bijarimi Iqbal, Anwar Ku Bulat, Ku Halim Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches |
title | Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches |
title_full | Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches |
title_fullStr | Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches |
title_full_unstemmed | Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches |
title_short | Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches |
title_sort | artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: rsm, cosmo rs, and molecular docking approaches |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293230/ https://www.ncbi.nlm.nih.gov/pubmed/32533034 http://dx.doi.org/10.1038/s41598-020-66488-7 |
work_keys_str_mv | AT ahmadmohammadnorazmi artocarpusaltilisextractsasafoodbornepathogenandoxidationinhibitorsrsmcosmorsandmoleculardockingapproaches AT karimnazatulumira artocarpusaltilisextractsasafoodbornepathogenandoxidationinhibitorsrsmcosmorsandmoleculardockingapproaches AT normayaerna artocarpusaltilisextractsasafoodbornepathogenandoxidationinhibitorsrsmcosmorsandmoleculardockingapproaches AT matpiahbijarimi artocarpusaltilisextractsasafoodbornepathogenandoxidationinhibitorsrsmcosmorsandmoleculardockingapproaches AT iqbalanwar artocarpusaltilisextractsasafoodbornepathogenandoxidationinhibitorsrsmcosmorsandmoleculardockingapproaches AT kubulatkuhalim artocarpusaltilisextractsasafoodbornepathogenandoxidationinhibitorsrsmcosmorsandmoleculardockingapproaches |