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Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated coronavirus disease 2019 (COVID-19) pandemic has demanded rapid upscaling of in-vitro diagnostic assays to enable mass screening and testing of high-risk groups, and simultaneous ascertainment of robust data on past...

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Autores principales: La Marca, Antonio, Capuzzo, Martina, Paglia, Tiziana, Roli, Laura, Trenti, Tommaso, Nelson, Scott M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd on behalf of Reproductive Healthcare Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293848/
https://www.ncbi.nlm.nih.gov/pubmed/32651106
http://dx.doi.org/10.1016/j.rbmo.2020.06.001
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author La Marca, Antonio
Capuzzo, Martina
Paglia, Tiziana
Roli, Laura
Trenti, Tommaso
Nelson, Scott M.
author_facet La Marca, Antonio
Capuzzo, Martina
Paglia, Tiziana
Roli, Laura
Trenti, Tommaso
Nelson, Scott M.
author_sort La Marca, Antonio
collection PubMed
description The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated coronavirus disease 2019 (COVID-19) pandemic has demanded rapid upscaling of in-vitro diagnostic assays to enable mass screening and testing of high-risk groups, and simultaneous ascertainment of robust data on past SARS-CoV-2 exposure at an individual and a population level. To meet the exponential demand in testing, there has been an accelerated development of both molecular and serological assays across a plethora of platforms. The present review discusses the current literature on these modalities, including nucleic acid amplification tests, direct viral antigen tests and the rapidly expanding laboratory-based and point of care serological tests. This suite of complementary tests will inform crucial decisions by healthcare providers and policy makers, and understanding their strengths and limitations will be critical to their judicious application for the development of algorithmic approaches to treatment and public health strategies.
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spelling pubmed-72938482020-06-15 Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays La Marca, Antonio Capuzzo, Martina Paglia, Tiziana Roli, Laura Trenti, Tommaso Nelson, Scott M. Reprod Biomed Online Review The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated coronavirus disease 2019 (COVID-19) pandemic has demanded rapid upscaling of in-vitro diagnostic assays to enable mass screening and testing of high-risk groups, and simultaneous ascertainment of robust data on past SARS-CoV-2 exposure at an individual and a population level. To meet the exponential demand in testing, there has been an accelerated development of both molecular and serological assays across a plethora of platforms. The present review discusses the current literature on these modalities, including nucleic acid amplification tests, direct viral antigen tests and the rapidly expanding laboratory-based and point of care serological tests. This suite of complementary tests will inform crucial decisions by healthcare providers and policy makers, and understanding their strengths and limitations will be critical to their judicious application for the development of algorithmic approaches to treatment and public health strategies. Published by Elsevier Ltd on behalf of Reproductive Healthcare Ltd. 2020-09 2020-06-14 /pmc/articles/PMC7293848/ /pubmed/32651106 http://dx.doi.org/10.1016/j.rbmo.2020.06.001 Text en Crown Copyright © 2020 Published by Elsevier Ltd on behalf of Reproductive Healthcare Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Review
La Marca, Antonio
Capuzzo, Martina
Paglia, Tiziana
Roli, Laura
Trenti, Tommaso
Nelson, Scott M.
Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays
title Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays
title_full Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays
title_fullStr Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays
title_full_unstemmed Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays
title_short Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays
title_sort testing for sars-cov-2 (covid-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293848/
https://www.ncbi.nlm.nih.gov/pubmed/32651106
http://dx.doi.org/10.1016/j.rbmo.2020.06.001
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