Cargando…
Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella
In order to enhance the production of glucose oxidase (GOD), random mutagenesis of Aspergillus tubingensis CTM 507 was performed using the chemical and physical mutagens: nitric acid and UV irradiation, respectively. The majority of the isolated mutants showed good GOD production, but only some muta...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294356/ https://www.ncbi.nlm.nih.gov/pubmed/32596402 http://dx.doi.org/10.1155/2020/9716581 |
_version_ | 1783546471960805376 |
---|---|
author | Kriaa, Mouna Boukedi, Hanen Ben Rhouma, Marwa Ben Nasr, Yosri Tounsi, Slim Mellouli, Lotfi Kammoun, Radhouane |
author_facet | Kriaa, Mouna Boukedi, Hanen Ben Rhouma, Marwa Ben Nasr, Yosri Tounsi, Slim Mellouli, Lotfi Kammoun, Radhouane |
author_sort | Kriaa, Mouna |
collection | PubMed |
description | In order to enhance the production of glucose oxidase (GOD), random mutagenesis of Aspergillus tubingensis CTM 507 was performed using the chemical and physical mutagens: nitric acid and UV irradiation, respectively. The majority of the isolated mutants showed good GOD production, but only some mutants presented a significant overproduction, as compared with the parent strain. The selected mutants (19 strains), showing an overproduction larger than 200%, are quite stable after three successive subcultures. Among these, six strains revealed an important improvement in submerged fermentation. The insecticidal activity of GOD produced by the wild and the selected mutant strains was evaluated against the third larval instars of E. kuehniella. Mutant strains U11, U12, U20, and U21, presenting the most important effect, displayed an LC(50) value of 89.00, 88.51, 80.00, and 86.00 U/cm(2), respectively, which was 1.5-fold more important than the wild strain (61 U/cm(2)). According to histopathology observations, the GOD enzyme showed approximately similar damage on the E. kuehniella midgut including rupture and disintegration of the epithelial layer and cellular vacuolization. The data supports, for the first time, the use of GOD as a pest control agent against E. kuehniella. |
format | Online Article Text |
id | pubmed-7294356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-72943562020-06-27 Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella Kriaa, Mouna Boukedi, Hanen Ben Rhouma, Marwa Ben Nasr, Yosri Tounsi, Slim Mellouli, Lotfi Kammoun, Radhouane Biomed Res Int Research Article In order to enhance the production of glucose oxidase (GOD), random mutagenesis of Aspergillus tubingensis CTM 507 was performed using the chemical and physical mutagens: nitric acid and UV irradiation, respectively. The majority of the isolated mutants showed good GOD production, but only some mutants presented a significant overproduction, as compared with the parent strain. The selected mutants (19 strains), showing an overproduction larger than 200%, are quite stable after three successive subcultures. Among these, six strains revealed an important improvement in submerged fermentation. The insecticidal activity of GOD produced by the wild and the selected mutant strains was evaluated against the third larval instars of E. kuehniella. Mutant strains U11, U12, U20, and U21, presenting the most important effect, displayed an LC(50) value of 89.00, 88.51, 80.00, and 86.00 U/cm(2), respectively, which was 1.5-fold more important than the wild strain (61 U/cm(2)). According to histopathology observations, the GOD enzyme showed approximately similar damage on the E. kuehniella midgut including rupture and disintegration of the epithelial layer and cellular vacuolization. The data supports, for the first time, the use of GOD as a pest control agent against E. kuehniella. Hindawi 2020-06-05 /pmc/articles/PMC7294356/ /pubmed/32596402 http://dx.doi.org/10.1155/2020/9716581 Text en Copyright © 2020 Mouna Kriaa et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kriaa, Mouna Boukedi, Hanen Ben Rhouma, Marwa Ben Nasr, Yosri Tounsi, Slim Mellouli, Lotfi Kammoun, Radhouane Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella |
title | Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella |
title_full | Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella |
title_fullStr | Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella |
title_full_unstemmed | Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella |
title_short | Overproduction of Glucose Oxidase by Aspergillus tubingensis CTM 507 Randomly Obtained Mutants and Study of Its Insecticidal Activity against Ephestia kuehniella |
title_sort | overproduction of glucose oxidase by aspergillus tubingensis ctm 507 randomly obtained mutants and study of its insecticidal activity against ephestia kuehniella |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294356/ https://www.ncbi.nlm.nih.gov/pubmed/32596402 http://dx.doi.org/10.1155/2020/9716581 |
work_keys_str_mv | AT kriaamouna overproductionofglucoseoxidasebyaspergillustubingensisctm507randomlyobtainedmutantsandstudyofitsinsecticidalactivityagainstephestiakuehniella AT boukedihanen overproductionofglucoseoxidasebyaspergillustubingensisctm507randomlyobtainedmutantsandstudyofitsinsecticidalactivityagainstephestiakuehniella AT benrhoumamarwa overproductionofglucoseoxidasebyaspergillustubingensisctm507randomlyobtainedmutantsandstudyofitsinsecticidalactivityagainstephestiakuehniella AT bennasryosri overproductionofglucoseoxidasebyaspergillustubingensisctm507randomlyobtainedmutantsandstudyofitsinsecticidalactivityagainstephestiakuehniella AT tounsislim overproductionofglucoseoxidasebyaspergillustubingensisctm507randomlyobtainedmutantsandstudyofitsinsecticidalactivityagainstephestiakuehniella AT melloulilotfi overproductionofglucoseoxidasebyaspergillustubingensisctm507randomlyobtainedmutantsandstudyofitsinsecticidalactivityagainstephestiakuehniella AT kammounradhouane overproductionofglucoseoxidasebyaspergillustubingensisctm507randomlyobtainedmutantsandstudyofitsinsecticidalactivityagainstephestiakuehniella |