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ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer

Breast cancer (BC) is one of the most common female cancers, and its incidence has been increasing in recent years. Although treatments are continuously improving, the prognosis of patients in the advanced stage is still unsatisfactory. Thus, an in-depth understanding of its molecular mechanisms is...

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Autores principales: Zeng, Huijuan, Li, Tianfu, Zhai, Duanyang, Bi, Jiong, Kuang, Xiaying, Lu, Sihong, Shan, Zhen, Lin, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294947/
https://www.ncbi.nlm.nih.gov/pubmed/32549756
http://dx.doi.org/10.7150/ijbs.44204
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author Zeng, Huijuan
Li, Tianfu
Zhai, Duanyang
Bi, Jiong
Kuang, Xiaying
Lu, Sihong
Shan, Zhen
Lin, Ying
author_facet Zeng, Huijuan
Li, Tianfu
Zhai, Duanyang
Bi, Jiong
Kuang, Xiaying
Lu, Sihong
Shan, Zhen
Lin, Ying
author_sort Zeng, Huijuan
collection PubMed
description Breast cancer (BC) is one of the most common female cancers, and its incidence has been increasing in recent years. Although treatments are continuously improving, the prognosis of patients in the advanced stage is still unsatisfactory. Thus, an in-depth understanding of its molecular mechanisms is necessary for curing breast cancer. KIF15 is a tetrameric spindle motor which can regulate mitosis in cellular process and exert the crucial functions in several cancers. The purpose of our research was to investigate the functions of KIF15 in breast cancer. We tested the expression of KIF15 in breast cancer tissues and the survival rate of breast cancer patients with high or low level of KIF15 through TCGA data. What's more, western blot and immunohistochemistry assay were utilized to evaluate the protein level and mRNA level of KIF15 in breast cancer tissues. Then CCK-8, wound healing, transwell and flow cytometry experiments were adopted separately to test cell viability, migration, invasion and cell cycle distribution. We discovered that KIF15 was highly expressed in breast cancer tissues and high level KIF15 was associated with a low survival rate of breast cancer patients. Moreover, silence of KIF15 suppressed cell viability, migration, invasion and cell cycle distribution. Following, we discovered that ZNF367 was the upstream transcription factor of KIF15. In addition, silenced ZNF367 could also repress the growth of breast cancer cells. And rescue experiments indicated that overexpressed KIF15 could counteract the inhibition effect of silencing ZNF367 on the progression of breast cancer. Importantly, we discovered that KIF15 and ZNF367 were associated with the regulation of cell cycle. In short, ZNF367-activated KIF15 accelerated the progression of breast cancer by regulating cell cycle progress.
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spelling pubmed-72949472020-06-16 ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer Zeng, Huijuan Li, Tianfu Zhai, Duanyang Bi, Jiong Kuang, Xiaying Lu, Sihong Shan, Zhen Lin, Ying Int J Biol Sci Research Paper Breast cancer (BC) is one of the most common female cancers, and its incidence has been increasing in recent years. Although treatments are continuously improving, the prognosis of patients in the advanced stage is still unsatisfactory. Thus, an in-depth understanding of its molecular mechanisms is necessary for curing breast cancer. KIF15 is a tetrameric spindle motor which can regulate mitosis in cellular process and exert the crucial functions in several cancers. The purpose of our research was to investigate the functions of KIF15 in breast cancer. We tested the expression of KIF15 in breast cancer tissues and the survival rate of breast cancer patients with high or low level of KIF15 through TCGA data. What's more, western blot and immunohistochemistry assay were utilized to evaluate the protein level and mRNA level of KIF15 in breast cancer tissues. Then CCK-8, wound healing, transwell and flow cytometry experiments were adopted separately to test cell viability, migration, invasion and cell cycle distribution. We discovered that KIF15 was highly expressed in breast cancer tissues and high level KIF15 was associated with a low survival rate of breast cancer patients. Moreover, silence of KIF15 suppressed cell viability, migration, invasion and cell cycle distribution. Following, we discovered that ZNF367 was the upstream transcription factor of KIF15. In addition, silenced ZNF367 could also repress the growth of breast cancer cells. And rescue experiments indicated that overexpressed KIF15 could counteract the inhibition effect of silencing ZNF367 on the progression of breast cancer. Importantly, we discovered that KIF15 and ZNF367 were associated with the regulation of cell cycle. In short, ZNF367-activated KIF15 accelerated the progression of breast cancer by regulating cell cycle progress. Ivyspring International Publisher 2020-05-16 /pmc/articles/PMC7294947/ /pubmed/32549756 http://dx.doi.org/10.7150/ijbs.44204 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Zeng, Huijuan
Li, Tianfu
Zhai, Duanyang
Bi, Jiong
Kuang, Xiaying
Lu, Sihong
Shan, Zhen
Lin, Ying
ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer
title ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer
title_full ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer
title_fullStr ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer
title_full_unstemmed ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer
title_short ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer
title_sort znf367-induced transcriptional activation of kif15 accelerates the progression of breast cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294947/
https://www.ncbi.nlm.nih.gov/pubmed/32549756
http://dx.doi.org/10.7150/ijbs.44204
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