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Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway

INTRODUCTION: Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. In the present research, we explored a new oncogene, derlin-1 (DERL1), and studied its role and mechanism in human HCC. METHODS: We assessed the expression and prognosis value of DERL1 in human HCC by...

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Autores principales: Fan, Jiye, Tian, Liying, Huang, Shuhong, Zhang, Jing, Zhao, Baohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295458/
https://www.ncbi.nlm.nih.gov/pubmed/32606758
http://dx.doi.org/10.2147/OTT.S222895
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author Fan, Jiye
Tian, Liying
Huang, Shuhong
Zhang, Jing
Zhao, Baohua
author_facet Fan, Jiye
Tian, Liying
Huang, Shuhong
Zhang, Jing
Zhao, Baohua
author_sort Fan, Jiye
collection PubMed
description INTRODUCTION: Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. In the present research, we explored a new oncogene, derlin-1 (DERL1), and studied its role and mechanism in human HCC. METHODS: We assessed the expression and prognosis value of DERL1 in human HCC by using GEPIA dataset analysis and immunohistochemistry. To elucidate the specific function of DERL1, we suppressed its expression in two HCC cell lines, HuH7 and Hep3B, and overexpressed DERL1 in Hep3B cells. Cell proliferation and migration was detected by CCK8 and transwell assays. Cell flow cytometry was used to evaluate cell apoptosis. RESULTS: Our results demonstrated that DERL1 was highly expressed in HCC samples (n = 369) than in normal samples (n = 160). Similar results were obtained in 60 clinical samples that we collected from the local hospital. The high expression rate of DERL1 reached 78.3% (47/60). DERL1 overexpression samples were concentrated in patients with tumor diameters >5cm or lymph node metastases. Thus, we speculated that DERL1 operated as a tumor promotor in HCC, and its expression might be proposed as a predictor for tumor metastasis of human HCC. Interference of DERL1 markedly blocked cell proliferation and migration, and induced the apoptosis of HCC cells in vitro. Phosphorylation of Akt was significantly inhibited in cells transfected with DERL1 siRNA compared to their control cells in HuH7 and Hep3B cell lines. The opposite result was observed in the DERL1 overexpression cells. CONCLUSION: Our findings prove that DERL1 promotes tumor progression via AKT pathway and provide a new potential target for the clinical treatment and diagnosis of human HCC.
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spelling pubmed-72954582020-06-29 Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway Fan, Jiye Tian, Liying Huang, Shuhong Zhang, Jing Zhao, Baohua Onco Targets Ther Original Research INTRODUCTION: Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. In the present research, we explored a new oncogene, derlin-1 (DERL1), and studied its role and mechanism in human HCC. METHODS: We assessed the expression and prognosis value of DERL1 in human HCC by using GEPIA dataset analysis and immunohistochemistry. To elucidate the specific function of DERL1, we suppressed its expression in two HCC cell lines, HuH7 and Hep3B, and overexpressed DERL1 in Hep3B cells. Cell proliferation and migration was detected by CCK8 and transwell assays. Cell flow cytometry was used to evaluate cell apoptosis. RESULTS: Our results demonstrated that DERL1 was highly expressed in HCC samples (n = 369) than in normal samples (n = 160). Similar results were obtained in 60 clinical samples that we collected from the local hospital. The high expression rate of DERL1 reached 78.3% (47/60). DERL1 overexpression samples were concentrated in patients with tumor diameters >5cm or lymph node metastases. Thus, we speculated that DERL1 operated as a tumor promotor in HCC, and its expression might be proposed as a predictor for tumor metastasis of human HCC. Interference of DERL1 markedly blocked cell proliferation and migration, and induced the apoptosis of HCC cells in vitro. Phosphorylation of Akt was significantly inhibited in cells transfected with DERL1 siRNA compared to their control cells in HuH7 and Hep3B cell lines. The opposite result was observed in the DERL1 overexpression cells. CONCLUSION: Our findings prove that DERL1 promotes tumor progression via AKT pathway and provide a new potential target for the clinical treatment and diagnosis of human HCC. Dove 2020-06-11 /pmc/articles/PMC7295458/ /pubmed/32606758 http://dx.doi.org/10.2147/OTT.S222895 Text en © 2020 Fan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Fan, Jiye
Tian, Liying
Huang, Shuhong
Zhang, Jing
Zhao, Baohua
Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway
title Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway
title_full Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway
title_fullStr Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway
title_full_unstemmed Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway
title_short Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway
title_sort derlin-1 promotes the progression of human hepatocellular carcinoma via the activation of akt pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295458/
https://www.ncbi.nlm.nih.gov/pubmed/32606758
http://dx.doi.org/10.2147/OTT.S222895
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