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Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response
Pacu (Piaractus mesopotamicus) is a Neotropical fish of major importance for South American aquaculture. Septicemia caused by Aeromonas hydrophila bacteria is currently considered a substantial threat for pacu aquaculture that have provoked infectious disease outbreaks with high economic losses. The...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295981/ https://www.ncbi.nlm.nih.gov/pubmed/32582300 http://dx.doi.org/10.3389/fgene.2020.00604 |
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author | Mastrochirico-Filho, Vito Antonio Hata, Milene Elissa Kuradomi, Rafael Yutaka de Freitas, Milena Vieira Ariede, Raquel Belini Pinheiro, Daniel Guariz Robledo, Diego Houston, Ross Hashimoto, Diogo Teruo |
author_facet | Mastrochirico-Filho, Vito Antonio Hata, Milene Elissa Kuradomi, Rafael Yutaka de Freitas, Milena Vieira Ariede, Raquel Belini Pinheiro, Daniel Guariz Robledo, Diego Houston, Ross Hashimoto, Diogo Teruo |
author_sort | Mastrochirico-Filho, Vito Antonio |
collection | PubMed |
description | Pacu (Piaractus mesopotamicus) is a Neotropical fish of major importance for South American aquaculture. Septicemia caused by Aeromonas hydrophila bacteria is currently considered a substantial threat for pacu aquaculture that have provoked infectious disease outbreaks with high economic losses. The understanding of molecular aspects on progress of A. hydrophila infection and pacu immune response is scarce, which have limited the development of genomic selection for resistance to this infection. The present study aimed to generate information on transcriptome of pacu in face of A. hydrophila infection, and compare the transcriptomic responses between two groups of time-series belonging to a disease resistance challenge, peak mortality (HM) and mortality plateau (PM) groups of individuals. Nine RNA sequencing (RNA-Seq) libraries were prepared from liver tissue of challenged individuals, generating ∼160 million 150 bp pair-end reads. After quality trimming/cleanup, these reads were assembled de novo generating 211,259 contigs. When the expression of genes from individuals of HM group were compared to individuals from control group, a total of 4,413 differentially expressed transcripts were found (2,000 upregulated and 2,413 downregulated candidate genes). Additionally, 433 transcripts were differentially expressed when individuals from MP group were compared with those in the control group (155 upregulated and 278 downregulated candidate genes). The resulting differentially expressed transcripts were clustered into the following functional categories: cytokines and signaling, epithelial protection, antigen processing and presentation, apoptosis, phagocytosis, complement system cascades and pattern recognition receptors. The proposed results revealing relevant differential gene expression on HM and PM groups which will contribute to a better understanding of the molecular defense mechanisms during A. hydrophila infection. |
format | Online Article Text |
id | pubmed-7295981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72959812020-06-23 Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response Mastrochirico-Filho, Vito Antonio Hata, Milene Elissa Kuradomi, Rafael Yutaka de Freitas, Milena Vieira Ariede, Raquel Belini Pinheiro, Daniel Guariz Robledo, Diego Houston, Ross Hashimoto, Diogo Teruo Front Genet Genetics Pacu (Piaractus mesopotamicus) is a Neotropical fish of major importance for South American aquaculture. Septicemia caused by Aeromonas hydrophila bacteria is currently considered a substantial threat for pacu aquaculture that have provoked infectious disease outbreaks with high economic losses. The understanding of molecular aspects on progress of A. hydrophila infection and pacu immune response is scarce, which have limited the development of genomic selection for resistance to this infection. The present study aimed to generate information on transcriptome of pacu in face of A. hydrophila infection, and compare the transcriptomic responses between two groups of time-series belonging to a disease resistance challenge, peak mortality (HM) and mortality plateau (PM) groups of individuals. Nine RNA sequencing (RNA-Seq) libraries were prepared from liver tissue of challenged individuals, generating ∼160 million 150 bp pair-end reads. After quality trimming/cleanup, these reads were assembled de novo generating 211,259 contigs. When the expression of genes from individuals of HM group were compared to individuals from control group, a total of 4,413 differentially expressed transcripts were found (2,000 upregulated and 2,413 downregulated candidate genes). Additionally, 433 transcripts were differentially expressed when individuals from MP group were compared with those in the control group (155 upregulated and 278 downregulated candidate genes). The resulting differentially expressed transcripts were clustered into the following functional categories: cytokines and signaling, epithelial protection, antigen processing and presentation, apoptosis, phagocytosis, complement system cascades and pattern recognition receptors. The proposed results revealing relevant differential gene expression on HM and PM groups which will contribute to a better understanding of the molecular defense mechanisms during A. hydrophila infection. Frontiers Media S.A. 2020-06-09 /pmc/articles/PMC7295981/ /pubmed/32582300 http://dx.doi.org/10.3389/fgene.2020.00604 Text en Copyright © 2020 Mastrochirico-Filho, Hata, Kuradomi, de Freitas, Ariede, Pinheiro, Robledo, Houston and Hashimoto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Mastrochirico-Filho, Vito Antonio Hata, Milene Elissa Kuradomi, Rafael Yutaka de Freitas, Milena Vieira Ariede, Raquel Belini Pinheiro, Daniel Guariz Robledo, Diego Houston, Ross Hashimoto, Diogo Teruo Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response |
title | Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response |
title_full | Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response |
title_fullStr | Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response |
title_full_unstemmed | Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response |
title_short | Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response |
title_sort | transcriptome profiling of pacu (piaractus mesopotamicus) challenged with pathogenic aeromonas hydrophila: inference on immune gene response |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295981/ https://www.ncbi.nlm.nih.gov/pubmed/32582300 http://dx.doi.org/10.3389/fgene.2020.00604 |
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