Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses

rgsCaM has been reported as a calmodulin-like (CML) factor induced by viral infection in Nicotiana. There are three CMLs that belong to the rgsCaM family in Arabidopsis thaliana. In this study, we found a total of 5 NbrgsCaM coding sequences in N. benthamiana genome. We analyzed transcription patter...

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Autores principales: Liu, Dandan, Yang, Qiuying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296017/
https://www.ncbi.nlm.nih.gov/pubmed/32541846
http://dx.doi.org/10.1038/s41598-020-66670-x
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author Liu, Dandan
Yang, Qiuying
author_facet Liu, Dandan
Yang, Qiuying
author_sort Liu, Dandan
collection PubMed
description rgsCaM has been reported as a calmodulin-like (CML) factor induced by viral infection in Nicotiana. There are three CMLs that belong to the rgsCaM family in Arabidopsis thaliana. In this study, we found a total of 5 NbrgsCaM coding sequences in N. benthamiana genome. We analyzed transcription patterns of NbrgsCaMs in transgenic plants expressing a β-glucuronidase (GUS) under the promoter of NbrgsCaMs by histochemistry staining and RT-qPCR. Similar to their Arabidopsis homologs, most NbrgsCaMs have an overlapping but distinct expression pattern in response to developmental and environmental changes. Specifically, the NbrgsCaM4 promoter exhibited robust activity and showed distinct regulatory response to viral infection, developmental stages and other abiotic stimuli. Overall, these findings provide clues for further understanding of the NbrgsCaM family genes in regulating plant growth and development under biotic stress and environmental stimulation.
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spelling pubmed-72960172020-06-17 Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses Liu, Dandan Yang, Qiuying Sci Rep Article rgsCaM has been reported as a calmodulin-like (CML) factor induced by viral infection in Nicotiana. There are three CMLs that belong to the rgsCaM family in Arabidopsis thaliana. In this study, we found a total of 5 NbrgsCaM coding sequences in N. benthamiana genome. We analyzed transcription patterns of NbrgsCaMs in transgenic plants expressing a β-glucuronidase (GUS) under the promoter of NbrgsCaMs by histochemistry staining and RT-qPCR. Similar to their Arabidopsis homologs, most NbrgsCaMs have an overlapping but distinct expression pattern in response to developmental and environmental changes. Specifically, the NbrgsCaM4 promoter exhibited robust activity and showed distinct regulatory response to viral infection, developmental stages and other abiotic stimuli. Overall, these findings provide clues for further understanding of the NbrgsCaM family genes in regulating plant growth and development under biotic stress and environmental stimulation. Nature Publishing Group UK 2020-06-15 /pmc/articles/PMC7296017/ /pubmed/32541846 http://dx.doi.org/10.1038/s41598-020-66670-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Dandan
Yang, Qiuying
Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses
title Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses
title_full Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses
title_fullStr Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses
title_full_unstemmed Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses
title_short Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses
title_sort expression patterns of nbrgscam family genes in nicotiana benthamiana and their potential roles in development and stress responses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296017/
https://www.ncbi.nlm.nih.gov/pubmed/32541846
http://dx.doi.org/10.1038/s41598-020-66670-x
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AT yangqiuying expressionpatternsofnbrgscamfamilygenesinnicotianabenthamianaandtheirpotentialrolesindevelopmentandstressresponses

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