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Structure and mechanism of DNA delivery of a gene transfer agent

Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of...

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Autores principales: Bárdy, Pavol, Füzik, Tibor, Hrebík, Dominik, Pantůček, Roman, Thomas Beatty, J., Plevka, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296036/
https://www.ncbi.nlm.nih.gov/pubmed/32541663
http://dx.doi.org/10.1038/s41467-020-16669-9
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author Bárdy, Pavol
Füzik, Tibor
Hrebík, Dominik
Pantůček, Roman
Thomas Beatty, J.
Plevka, Pavel
author_facet Bárdy, Pavol
Füzik, Tibor
Hrebík, Dominik
Pantůček, Roman
Thomas Beatty, J.
Plevka, Pavel
author_sort Bárdy, Pavol
collection PubMed
description Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm.
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spelling pubmed-72960362020-06-19 Structure and mechanism of DNA delivery of a gene transfer agent Bárdy, Pavol Füzik, Tibor Hrebík, Dominik Pantůček, Roman Thomas Beatty, J. Plevka, Pavel Nat Commun Article Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. Nature Publishing Group UK 2020-06-15 /pmc/articles/PMC7296036/ /pubmed/32541663 http://dx.doi.org/10.1038/s41467-020-16669-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bárdy, Pavol
Füzik, Tibor
Hrebík, Dominik
Pantůček, Roman
Thomas Beatty, J.
Plevka, Pavel
Structure and mechanism of DNA delivery of a gene transfer agent
title Structure and mechanism of DNA delivery of a gene transfer agent
title_full Structure and mechanism of DNA delivery of a gene transfer agent
title_fullStr Structure and mechanism of DNA delivery of a gene transfer agent
title_full_unstemmed Structure and mechanism of DNA delivery of a gene transfer agent
title_short Structure and mechanism of DNA delivery of a gene transfer agent
title_sort structure and mechanism of dna delivery of a gene transfer agent
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296036/
https://www.ncbi.nlm.nih.gov/pubmed/32541663
http://dx.doi.org/10.1038/s41467-020-16669-9
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