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A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion

Many obligate intracellular apicomplexan parasites have adapted a distinct invasion mechanism involving a close interaction between the parasite ligands and the sialic acid (SA) receptor. We found that sialic acid binding protein-1 (SABP1), localized on the outer membrane of the zoonotic parasite To...

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Autores principales: Xing, Mengen, Yang, Na, Jiang, Ning, Wang, Dawei, Sang, Xiaoyu, Feng, Ying, Chen, Ran, Wang, Xinyi, Chen, Qijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296849/
https://www.ncbi.nlm.nih.gov/pubmed/32060530
http://dx.doi.org/10.1093/infdis/jiaa072
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author Xing, Mengen
Yang, Na
Jiang, Ning
Wang, Dawei
Sang, Xiaoyu
Feng, Ying
Chen, Ran
Wang, Xinyi
Chen, Qijun
author_facet Xing, Mengen
Yang, Na
Jiang, Ning
Wang, Dawei
Sang, Xiaoyu
Feng, Ying
Chen, Ran
Wang, Xinyi
Chen, Qijun
author_sort Xing, Mengen
collection PubMed
description Many obligate intracellular apicomplexan parasites have adapted a distinct invasion mechanism involving a close interaction between the parasite ligands and the sialic acid (SA) receptor. We found that sialic acid binding protein-1 (SABP1), localized on the outer membrane of the zoonotic parasite Toxoplasma gondii, readily binds to sialic acid on the host cell surface. The binding was sensitive to neuraminidase treatment. Cells preincubated with recombinant SABP1 protein resisted parasite invasion in vitro. The parasite lost its invasion capacity and animal infectivity after the SABP1 gene was deleted, whereas complementation of the SABP1 gene restored the virulence of the knockout strain. These data establish the critical role of SABP1 in the invasion process of T. gondii. The previously uncharacterized protein, SABP1, facilitated T. gondii attachment and invasion via sialic acid receptors.
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spelling pubmed-72968492020-06-22 A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion Xing, Mengen Yang, Na Jiang, Ning Wang, Dawei Sang, Xiaoyu Feng, Ying Chen, Ran Wang, Xinyi Chen, Qijun J Infect Dis Major Articles and Brief Reports Many obligate intracellular apicomplexan parasites have adapted a distinct invasion mechanism involving a close interaction between the parasite ligands and the sialic acid (SA) receptor. We found that sialic acid binding protein-1 (SABP1), localized on the outer membrane of the zoonotic parasite Toxoplasma gondii, readily binds to sialic acid on the host cell surface. The binding was sensitive to neuraminidase treatment. Cells preincubated with recombinant SABP1 protein resisted parasite invasion in vitro. The parasite lost its invasion capacity and animal infectivity after the SABP1 gene was deleted, whereas complementation of the SABP1 gene restored the virulence of the knockout strain. These data establish the critical role of SABP1 in the invasion process of T. gondii. The previously uncharacterized protein, SABP1, facilitated T. gondii attachment and invasion via sialic acid receptors. Oxford University Press 2020-07-01 2020-02-15 /pmc/articles/PMC7296849/ /pubmed/32060530 http://dx.doi.org/10.1093/infdis/jiaa072 Text en © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Major Articles and Brief Reports
Xing, Mengen
Yang, Na
Jiang, Ning
Wang, Dawei
Sang, Xiaoyu
Feng, Ying
Chen, Ran
Wang, Xinyi
Chen, Qijun
A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
title A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
title_full A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
title_fullStr A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
title_full_unstemmed A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
title_short A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
title_sort sialic acid-binding protein sabp1 of toxoplasma gondii mediates host cell attachment and invasion
topic Major Articles and Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296849/
https://www.ncbi.nlm.nih.gov/pubmed/32060530
http://dx.doi.org/10.1093/infdis/jiaa072
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