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Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147
Bacterial infection of the kidney leads to a rapid cascade of host protective responses, many of which are still poorly understood. We have previously shown that following kidney infection with uropathogenicEscherichia coli (UPEC), vascular coagulation is quickly initiated in local perivascular capi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297223/ https://www.ncbi.nlm.nih.gov/pubmed/30476069 http://dx.doi.org/10.1093/femspd/fty087 |
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author | Schulz, Anette Chuquimia, Olga D Antypas, Haris Steiner, Svava E Sandoval, Ruben M Tanner, George A Molitoris, Bruce A Richter-Dahlfors, Agneta Melican, Keira |
author_facet | Schulz, Anette Chuquimia, Olga D Antypas, Haris Steiner, Svava E Sandoval, Ruben M Tanner, George A Molitoris, Bruce A Richter-Dahlfors, Agneta Melican, Keira |
author_sort | Schulz, Anette |
collection | PubMed |
description | Bacterial infection of the kidney leads to a rapid cascade of host protective responses, many of which are still poorly understood. We have previously shown that following kidney infection with uropathogenicEscherichia coli (UPEC), vascular coagulation is quickly initiated in local perivascular capillaries that protects the host from progressing from a local infection to systemic sepsis. The signaling mechanisms behind this response have not however been described. In this study, we use a number ofin vitro andin vivo techniques, including intravital microscopy, to identify two previously unrecognized components influencing this protective coagulation response. The acylation state of the Lipid A of UPEC lipopolysaccharide (LPS) is shown to alter the kinetics of local coagulation onsetin vivo. We also identify epithelial CD147 as a potential host factor influencing infection-mediated coagulation. CD147 is expressed by renal proximal epithelial cells infected with UPEC, contingent to bacterial expression of the α-hemolysin toxin. The epithelial CD147 subsequently can activate tissue factor on endothelial cells, a primary step in the coagulation cascade. This study emphasizes the rapid, multifaceted response of the kidney tissue to bacterial infection and the interplay between host and pathogen during the early hours of renal infection. |
format | Online Article Text |
id | pubmed-7297223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72972232020-06-22 Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147 Schulz, Anette Chuquimia, Olga D Antypas, Haris Steiner, Svava E Sandoval, Ruben M Tanner, George A Molitoris, Bruce A Richter-Dahlfors, Agneta Melican, Keira Pathog Dis Research Article Bacterial infection of the kidney leads to a rapid cascade of host protective responses, many of which are still poorly understood. We have previously shown that following kidney infection with uropathogenicEscherichia coli (UPEC), vascular coagulation is quickly initiated in local perivascular capillaries that protects the host from progressing from a local infection to systemic sepsis. The signaling mechanisms behind this response have not however been described. In this study, we use a number ofin vitro andin vivo techniques, including intravital microscopy, to identify two previously unrecognized components influencing this protective coagulation response. The acylation state of the Lipid A of UPEC lipopolysaccharide (LPS) is shown to alter the kinetics of local coagulation onsetin vivo. We also identify epithelial CD147 as a potential host factor influencing infection-mediated coagulation. CD147 is expressed by renal proximal epithelial cells infected with UPEC, contingent to bacterial expression of the α-hemolysin toxin. The epithelial CD147 subsequently can activate tissue factor on endothelial cells, a primary step in the coagulation cascade. This study emphasizes the rapid, multifaceted response of the kidney tissue to bacterial infection and the interplay between host and pathogen during the early hours of renal infection. Oxford University Press 2018-11-26 /pmc/articles/PMC7297223/ /pubmed/30476069 http://dx.doi.org/10.1093/femspd/fty087 Text en © FEMS 2018. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Schulz, Anette Chuquimia, Olga D Antypas, Haris Steiner, Svava E Sandoval, Ruben M Tanner, George A Molitoris, Bruce A Richter-Dahlfors, Agneta Melican, Keira Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147 |
title | Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147 |
title_full | Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147 |
title_fullStr | Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147 |
title_full_unstemmed | Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147 |
title_short | Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147 |
title_sort | protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid a and host cd147 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297223/ https://www.ncbi.nlm.nih.gov/pubmed/30476069 http://dx.doi.org/10.1093/femspd/fty087 |
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