A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing

Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and β-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and β-thalassemia. The ability of human embryonic stem cells (hES...

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Autores principales: Alexeeva, Vera, Aydin, Iraz T., Schaniel, Christoph, Stranahan, Alec W., D’Souza, Sunita L., Bieker, James J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297435/
https://www.ncbi.nlm.nih.gov/pubmed/32413789
http://dx.doi.org/10.1016/j.scr.2020.101837
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author Alexeeva, Vera
Aydin, Iraz T.
Schaniel, Christoph
Stranahan, Alec W.
D’Souza, Sunita L.
Bieker, James J.
author_facet Alexeeva, Vera
Aydin, Iraz T.
Schaniel, Christoph
Stranahan, Alec W.
D’Souza, Sunita L.
Bieker, James J.
author_sort Alexeeva, Vera
collection PubMed
description Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and β-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and β-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin.
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spelling pubmed-72974352020-06-16 A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing Alexeeva, Vera Aydin, Iraz T. Schaniel, Christoph Stranahan, Alec W. D’Souza, Sunita L. Bieker, James J. Stem Cell Res Article Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and β-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and β-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin. 2020-05-06 2020-05 /pmc/articles/PMC7297435/ /pubmed/32413789 http://dx.doi.org/10.1016/j.scr.2020.101837 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Alexeeva, Vera
Aydin, Iraz T.
Schaniel, Christoph
Stranahan, Alec W.
D’Souza, Sunita L.
Bieker, James J.
A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
title A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
title_full A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
title_fullStr A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
title_full_unstemmed A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
title_short A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
title_sort human h1-hbb11-gfp reporter embryonic stem cell line (wae001-a-2) generated using talen-based genome editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297435/
https://www.ncbi.nlm.nih.gov/pubmed/32413789
http://dx.doi.org/10.1016/j.scr.2020.101837
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