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Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus
Leaf color is an important characteristic of normal chloroplast development. Variegated plants have green- and white-sectored leaves, which can be used to identify important pathways and molecular mechanisms of chloroplast development. We studied two Brassica napus variegation mutants from same one...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297816/ https://www.ncbi.nlm.nih.gov/pubmed/32306107 http://dx.doi.org/10.1007/s00438-020-01674-w |
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author | Zhao, Xiaobin Hu, Kaining Yan, Mengjiao Yi, Bin Wen, Jing Ma, Chaozhi Shen, Jinxiong Fu, Tingdong Tu, Jinxing |
author_facet | Zhao, Xiaobin Hu, Kaining Yan, Mengjiao Yi, Bin Wen, Jing Ma, Chaozhi Shen, Jinxiong Fu, Tingdong Tu, Jinxing |
author_sort | Zhao, Xiaobin |
collection | PubMed |
description | Leaf color is an important characteristic of normal chloroplast development. Variegated plants have green- and white-sectored leaves, which can be used to identify important pathways and molecular mechanisms of chloroplast development. We studied two Brassica napus variegation mutants from same one variegated ancestor, designated ZY-4 and ZY-8, which have different degrees of variegation. When grown in identical conditions, the ratio of white sectors in ZY-4 leaves is higher than in ZY-8. In both mutants, the cells in green sectors contain normal chloroplasts; while, the cells in white sectors contain abnormal plastids. Seedling chloroplasts ultrastructure of both mutants showed that the biogenesis of chloroplasts was blocked in early stages; delayed development and structual damage in ZY-4 were more serious than in ZY-8. Employing bulked segregant analysis(BSA), two bulks (BY142 and BY137) from BC(2)F(1) lines derived from ZY-4 and ZS11, and one bulk (BY56) from BC(2)F(1) lines derived from ZY-8 and ZS11, and screening by Brassica 60K SNP BeadChip Array, showed the candidate regions localized in chromosome A08 (BY142), C04 (BY137), and A08 (BY56), respectively. Transcriptome analysis of five seedling development stages of ZY-4, ZY-8, and ZS11 showed that photosynthesis, energy metabolism-related pathways and translation-related pathways were important for chloroplast biogenesis. The number of down- or up-regulated genes related to immune system process in ZY-4 was more than in ZY-8. The retrograde signaling pathway was mis-regulated in both mutants. DEG analysis indicated that both mutants showed photooxidative damages. By coupling transcriptome and BSA CHIP analyses, some candidate genes were identified. The gene expression pattern of carotene biosynthesis pathway was disrupted in both mutants. However, histochemical analysis of ROS revealed that there was no excessive accumulation of ROS in ZY-4 and ZY-8. Taken together, our data indicate that the disruption of carotene biosynthetic pathways leads to the variegation phenotypes of ZY-4 and ZY-8 and there are some functions that can compensate for the disruption of carotene biosynthesis in ZY-4 and ZY-8 to reduce ROS and prevent seedling mortality. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-020-01674-w) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7297816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-72978162020-06-19 Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus Zhao, Xiaobin Hu, Kaining Yan, Mengjiao Yi, Bin Wen, Jing Ma, Chaozhi Shen, Jinxiong Fu, Tingdong Tu, Jinxing Mol Genet Genomics Original Article Leaf color is an important characteristic of normal chloroplast development. Variegated plants have green- and white-sectored leaves, which can be used to identify important pathways and molecular mechanisms of chloroplast development. We studied two Brassica napus variegation mutants from same one variegated ancestor, designated ZY-4 and ZY-8, which have different degrees of variegation. When grown in identical conditions, the ratio of white sectors in ZY-4 leaves is higher than in ZY-8. In both mutants, the cells in green sectors contain normal chloroplasts; while, the cells in white sectors contain abnormal plastids. Seedling chloroplasts ultrastructure of both mutants showed that the biogenesis of chloroplasts was blocked in early stages; delayed development and structual damage in ZY-4 were more serious than in ZY-8. Employing bulked segregant analysis(BSA), two bulks (BY142 and BY137) from BC(2)F(1) lines derived from ZY-4 and ZS11, and one bulk (BY56) from BC(2)F(1) lines derived from ZY-8 and ZS11, and screening by Brassica 60K SNP BeadChip Array, showed the candidate regions localized in chromosome A08 (BY142), C04 (BY137), and A08 (BY56), respectively. Transcriptome analysis of five seedling development stages of ZY-4, ZY-8, and ZS11 showed that photosynthesis, energy metabolism-related pathways and translation-related pathways were important for chloroplast biogenesis. The number of down- or up-regulated genes related to immune system process in ZY-4 was more than in ZY-8. The retrograde signaling pathway was mis-regulated in both mutants. DEG analysis indicated that both mutants showed photooxidative damages. By coupling transcriptome and BSA CHIP analyses, some candidate genes were identified. The gene expression pattern of carotene biosynthesis pathway was disrupted in both mutants. However, histochemical analysis of ROS revealed that there was no excessive accumulation of ROS in ZY-4 and ZY-8. Taken together, our data indicate that the disruption of carotene biosynthetic pathways leads to the variegation phenotypes of ZY-4 and ZY-8 and there are some functions that can compensate for the disruption of carotene biosynthesis in ZY-4 and ZY-8 to reduce ROS and prevent seedling mortality. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-020-01674-w) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-04-18 2020 /pmc/articles/PMC7297816/ /pubmed/32306107 http://dx.doi.org/10.1007/s00438-020-01674-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Original Article Zhao, Xiaobin Hu, Kaining Yan, Mengjiao Yi, Bin Wen, Jing Ma, Chaozhi Shen, Jinxiong Fu, Tingdong Tu, Jinxing Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus |
title | Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus |
title_full | Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus |
title_fullStr | Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus |
title_full_unstemmed | Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus |
title_short | Disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in Brassica napus |
title_sort | disruption of carotene biosynthesis leads to abnormal plastids and variegated leaves in brassica napus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297816/ https://www.ncbi.nlm.nih.gov/pubmed/32306107 http://dx.doi.org/10.1007/s00438-020-01674-w |
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