NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish

While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventio...

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Autores principales: Mattern, Kai, Trotha, Jakob William von, Erfle, Peer, Köster, Reinhard Wolfgang, Dietzel, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298014/
https://www.ncbi.nlm.nih.gov/pubmed/32546816
http://dx.doi.org/10.1038/s42003-020-1029-7
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author Mattern, Kai
Trotha, Jakob William von
Erfle, Peer
Köster, Reinhard Wolfgang
Dietzel, Andreas
author_facet Mattern, Kai
Trotha, Jakob William von
Erfle, Peer
Köster, Reinhard Wolfgang
Dietzel, Andreas
author_sort Mattern, Kai
collection PubMed
description While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease.
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spelling pubmed-72980142020-06-22 NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish Mattern, Kai Trotha, Jakob William von Erfle, Peer Köster, Reinhard Wolfgang Dietzel, Andreas Commun Biol Article While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease. Nature Publishing Group UK 2020-06-16 /pmc/articles/PMC7298014/ /pubmed/32546816 http://dx.doi.org/10.1038/s42003-020-1029-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mattern, Kai
Trotha, Jakob William von
Erfle, Peer
Köster, Reinhard Wolfgang
Dietzel, Andreas
NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
title NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
title_full NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
title_fullStr NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
title_full_unstemmed NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
title_short NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
title_sort neuroexaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298014/
https://www.ncbi.nlm.nih.gov/pubmed/32546816
http://dx.doi.org/10.1038/s42003-020-1029-7
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