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Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis

Protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. Although several ubiquitinated proteins have been found in buffalo (Bubalus bubalis) testis in our previous studies, large-scale prof...

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Autores principales: Huang, Yu-lin, Zhang, Peng-fei, Hou, Zhen, Fu, Qiang, Li, Ming-xing, Huang, De-lun, Deng, Ting-xian, Lu, Yang-qing, Liang, Xian-wei, Zhang, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298129/
https://www.ncbi.nlm.nih.gov/pubmed/32469046
http://dx.doi.org/10.1042/BSR20193537
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author Huang, Yu-lin
Zhang, Peng-fei
Hou, Zhen
Fu, Qiang
Li, Ming-xing
Huang, De-lun
Deng, Ting-xian
Lu, Yang-qing
Liang, Xian-wei
Zhang, Ming
author_facet Huang, Yu-lin
Zhang, Peng-fei
Hou, Zhen
Fu, Qiang
Li, Ming-xing
Huang, De-lun
Deng, Ting-xian
Lu, Yang-qing
Liang, Xian-wei
Zhang, Ming
author_sort Huang, Yu-lin
collection PubMed
description Protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. Although several ubiquitinated proteins have been found in buffalo (Bubalus bubalis) testis in our previous studies, large-scale profiling of buffalo testis ubiquitome has not been reported to date. In the present study, we first identified a global profiling of lysine ubiquitination of adult buffalo testis using a highly sensitive LC-MS/MS coupled with immune-affinity enrichment of ubiquitinated peptides. In total, 422 lysine ubiquitination sites were identified in 262 proteins in adult buffalo testis tissue. Bioinformatics analysis showed that the ubiquitinated proteins are involved in a variety of biological processes and diverse subcellular localizations. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein interaction network analysis indicated that proteasome, glycolysis/gluconeogenesis and gap junction pathways are modulated by protein ubiquitination in testis. Besides, 44 ubiquitinated proteins may involve in spermatogenesis according to the SpermatogenesisOnline database, of which, the ubiquitination of HSPA2 and UCHL1 were confirmed by Immunoprecipitation (IP)/Western blot analysis. Taken together, these data provide a global view of ubiquitome in buffalo testis for the first time, and serve as an important resource for exploring the physiological role especially spermatogenesis of lysine ubiquitination in testis in mammals.
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spelling pubmed-72981292020-06-19 Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis Huang, Yu-lin Zhang, Peng-fei Hou, Zhen Fu, Qiang Li, Ming-xing Huang, De-lun Deng, Ting-xian Lu, Yang-qing Liang, Xian-wei Zhang, Ming Biosci Rep Bioinformatics Protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. Although several ubiquitinated proteins have been found in buffalo (Bubalus bubalis) testis in our previous studies, large-scale profiling of buffalo testis ubiquitome has not been reported to date. In the present study, we first identified a global profiling of lysine ubiquitination of adult buffalo testis using a highly sensitive LC-MS/MS coupled with immune-affinity enrichment of ubiquitinated peptides. In total, 422 lysine ubiquitination sites were identified in 262 proteins in adult buffalo testis tissue. Bioinformatics analysis showed that the ubiquitinated proteins are involved in a variety of biological processes and diverse subcellular localizations. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein interaction network analysis indicated that proteasome, glycolysis/gluconeogenesis and gap junction pathways are modulated by protein ubiquitination in testis. Besides, 44 ubiquitinated proteins may involve in spermatogenesis according to the SpermatogenesisOnline database, of which, the ubiquitination of HSPA2 and UCHL1 were confirmed by Immunoprecipitation (IP)/Western blot analysis. Taken together, these data provide a global view of ubiquitome in buffalo testis for the first time, and serve as an important resource for exploring the physiological role especially spermatogenesis of lysine ubiquitination in testis in mammals. Portland Press Ltd. 2020-06-16 /pmc/articles/PMC7298129/ /pubmed/32469046 http://dx.doi.org/10.1042/BSR20193537 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Bioinformatics
Huang, Yu-lin
Zhang, Peng-fei
Hou, Zhen
Fu, Qiang
Li, Ming-xing
Huang, De-lun
Deng, Ting-xian
Lu, Yang-qing
Liang, Xian-wei
Zhang, Ming
Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis
title Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis
title_full Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis
title_fullStr Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis
title_full_unstemmed Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis
title_short Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis
title_sort ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (bubalus bubalis) testis
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298129/
https://www.ncbi.nlm.nih.gov/pubmed/32469046
http://dx.doi.org/10.1042/BSR20193537
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